Search

Abstract Background Insulin resistance (IR) and serum uric acid (SUA) are closely interconnected: SUA contributes to adversely affects the insulin signaling pathway and contributes to IR, while IR is a known predictor for the development of hyperuricemia. The triglyceride (TG) to high-density lipoprotein cholesterol (HDL-C) ratio has been proposed as an easily obtainable marker for IR. This research aimed to investigate the interaction between IR and glomerular filtration rate (GFR)-adjusted uricemia (SUA/GFR ratio) in determining CV risk in a large population cohort study. Methods Data from 18,694 subjects were analyzed from Uric acid Right foR heArt Healt (URRAH) database. The study evaluated the association between TG/HDL-C ratio and SUA/GFR ratio, as well as their impact on the development of outcomes during the follow-up study period. The primary endpoint was CV mortality. Results After a mean follow-up of 124 ± 64 months, 2,665 (14.2%) CV deaths occurred. The incidence of fatal and non-fatal CV events increased in parallel with the increase of TG/HDL-C quintiles. TG/HDL-C ratio showed a positive association with increasing of SUA/GFR ratio, even in non-diabetic patients. Multivariate analysis showed that the TG/HDL-C ratio increases the mortality risk even after adjustment for potential confounding factors. Finally, IR and GFR-adjusted hyperuricemia showed an additive effect on CV mortality. Conclusions Both IR and SUA/GFR ratio independently predict CV mortality, regardless of age, gender, BMI, diabetes, hypertension and statin use. The joint effect of the TG/HDL-C ratio and the elevated SUA/GFR ratio was greater than the presence of each single risk factor on CV mortality. This highlights the importance of monitoring these markers to better assess cardiovascular risk.

Phenylethanoid glycosides (PhGs) are widely occurring secondary metabolites of medicinal plants with interesting biological activities such as antioxidant, anti-inflammatory, neuroprotective, antiviral, hepatoprotective, immunomodulatory, etc. They are characterized by a structural core formed by a phenethyl alcohol, usually tyrosol or hydroxytyrosol, attached to β-D-glucopyranose via a glycosidic bond. This core is usually further decorated by attached phenolic acids or another saccharide. Several studies suggest an important role of the saccharidic fragment in the biological activities of PhGs, provoking demand for new glycovariants of natural PhGs. This study presents the preparation of β-mannosylated analogs of tyrosol β-D-glucopyranoside (salidroside) and hydroxytyrosol β-D-glucopyranoside (hydroxysalidroside). While the chemical synthesis of β-D-mannopyranosides is rather challenging, they can be prepared by enzymatic catalysis. We found that Novozym 188, an industrial β-glucosidase, also contains β-mannosidase and used this enzyme in the preparation of tyrosol β-D-mannopyranoside and hydroxytyrosol β-D-mannopyranoside in 12 and 16% chemical yields, respectively, by transglycosylation from β-D-mannopyranosyl-(1→4)-D-mannose. The mannosylation was chemoselective and occurred exclusively on the primary hydroxyls of tyrosol and hydroxytyrosol, and the glycosylation of phenolic moieties of the aglycons was observed.