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Three Gram-stain-negative, aerobic, short rod-shaped and motile strains (FXH3W T , SHGZ20W and SMYT11W T ) were isolated from freshwater environments in China. Comparisons based on the 16S rRNA gene sequences indicated that strains FXH3W T and SHGZ20W showed the highest 16S rRNA gene sequence similarity of about 99.6% to ' Luteimonas cellulosilyticus ' MIC 1.5, and strain SMYT11W T showed the highest 16S rRNA gene sequence similarity of 99.8% to Luteimonas fraxinea D4P002 T , respectively. Observing the phylogenetic trees reconstructed based on the 16S rRNA gene sequences, the species of genera Luteimonas and Lysobacter were not monophyletic and often mixed together. The further reconstructed phylogenomic tree and Genome Taxonomy Database also showed that the species of both genera were polyphyletic, implying that the current taxonomic status of the species of both genera was questionable. The calculated OrthoANIu, digital DNA-DNA hybridization and average amino acid sequence identity (AAI) values supported that strains FXH3W T and SHGZ20W should belong to the same novel species and strain SMYT11W T should also represent an independent novel species. Combining the AAI values and phylogenomic analyses, the species of genera Luteimonas and Lysobacter should be reassigned to 12 genera ( Luteimonas , Lysobacter , Cognatiluteimonas , Noviluteimonas , Pseudoluteimonas , Solilutibacter , Agrilutibacter , Cognatilysobacter , Marilutibacter , Novilysobacter , Montanilutibacter and Aerolutibacter ). The AAI values 69.5-76.0% were also proposed as the Lysobacteraceae -specific thresholds for genus delineation. Strain SMYT11W T should represent a novel species of the genus Luteimonas , for which the name Luteimonas flava sp. nov. (type strains SMYT11W T =GDMCC 1.4275 T =KCTC 8304 T ) is proposed. Strains FXH3W T and SHGZ20W should represent a novel species of a new genus Aquilutibacter rugosus gen. nov., sp. nov. The type strain of the type species is FXH3W T (=GDMCC 1.4096 T =KCTC 8154 T ).

Two Gram-negative bacteria, designated as strains LF1 T and HM2-2 T , were isolated from an artificial pond in a honey farm at Hoengseong-gun, Gangwon-do, Republic of Korea. The 16S rRNA sequence analysis results revealed that strain LF1 T belonged to the genus Lysobacter and had the highest sequence similarity to Lysobacter niastensis GH41-7 T (99.0 %), Lysobacter panacisoli CJ29 T (98.9 %), and Lysobacter prati SYSU H10001 T (98.2 %). Its growth occurred at 20-37 °C, at pH 5.0-12.0, and in the presence of 0-2% NaCl. The major fatty acids were iso-C 15 : 0 , iso-C 16 : 0 , and summed feature 9 (iso-C 17 : 1  ω 9 c and/or C 16 : 0 10-methyl). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The DNA G+C content was 67.5 mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain LF1 T and species of the genus Lysobacter were 79.1-84.4% and 22.0-27.5 %, respectively. The 16S rRNA sequence analysis results revealed that strain HM2-2 T belonged to the genus Limnohabitans and was most closely related to Limnohabitans planktonicus II-D5 T (98.9 %), Limnohabitans radicicola JUR4 T (98.4%), and Limnohabitans parvus II-B4 T (98.4 %). Its growth occurred at 10-35 °C, at pH 5.0-11.0, and in the presence of 0-2% NaCl. The major fatty acids were C 16 : 0 and summed feature 3 (C 16 : 1  ω 7 c /C 16 : 1  ω 6 c ). The major polar lipid was phosphatidylethanolamine. The DNA G+C content was 59.9 mol%. The ANI and dDDH values between strain HM2-2 T and its closely related strains were 75.1-83.0% and 20.4-26.4 %, respectively. Phenotypic, genomic, and phylogenetic data revealed that strains LF1 T and HM2-2 T represent novel species in the genera Lysobacter and Limnohabitans , for which the names Lysobacter stagni sp. nov. and Limnohabitans lacus sp. nov. are proposed, respectively. The type strain of Lys. stagni is LF1 T (=KACC 23251 T =TBRC 17648 T ), and that of Lim. lacus is HM2-2 T (=KACC 23250 T =TBRC 17649 T ).