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Objective: Tumor immune infiltration leads to poor prognosis of gastric cancer patients and seriously affects the life quality of gastric cancer patients. This study was based on bioinformatics to screen prognostic biomarkers in patients with high degree of immune invasion of gastric cancer. Meanwhile, the action of biomarker CCDC80 was explored in gastric cancer by cell and tumorigenesis experiments, to provide reference for the cure of gastric cancer patients. Methods: Data sets and clinical massage on gastric cancer were collected from TCGA database and GEO database. ConsensusClusterPlus was used to cluster gastric cancer patients based on the 28 immune cells infiltration in ssGSEA. R "Limma" package was applied to analyze differential mRNAs between Cluster 1 and Cluster 2. Differential expression genes were screened by single factor analysis. Stemness markers (SERPINF1, DCN, CCDC80, FBLN5, SPARCL1, CCL14, DPYSL3) were identified for differential expression genes. Prognostic value of CCDC80 was evaluated in gastric cancer. Differences in genomic mutation and tumor microenvironment immune infiltration were assessed between high or low CCDC80. Finally, gastric cancer cells (HGC-27 and MKN-45) were selected to evaluate the action of silencing CCDC80 on malignant characterization, macrophage polarization, and tumor formation. Results: Bioinformatics analysis showed that CCDC80, as a stemness marker, was significantly overexpressed in gastric cancer. CCDC80 was also related to the degree of gastric cancer immune invasion. CCDC80 was up-expressed in cells of gastric cancer. Silencing CCDC80 inhibited malignant characterization and subcutaneous tumor formation of gastric cancer cells. High expression of CCDC80 was positive correspondence with immune invasion. Silencing CCDC80 inhibited M2 polarization and promoted M1 polarization in tumor tissues. In addition, gastric cancer patients were likely to have mutations in CDH1, ACTRT1, GANAB, and CDH10 genes in the High-CCDC80 group. Conclusion: Silencing CCDC80, a prognostic biomarker in patients with immune invasion of gastric cancer, could effectively inhibit the malignant characterization, M2 polarization, and tumor formation of gastric cancer. [ABSTRACT FROM AUTHOR]

Zirconia implants have great application prospects due to their favorable biocompatibility and esthetic properties. However, the percentage of osseointegration of zirconia implants is lower than that of titanium implants. This study, structures bioactive surfaces on zirconia using a femtosecond laser combined with hydroxyapatite (HA) crystals and evaluates the surface morphology, functional groups, crystal phase, mechanical performance, and cell response. The results revealed that calcium phosphate deposition coincided with the establishment of micro‐grooves on the zirconia surface. The crystal composition and flexural strength of zirconia showed no significant changes after the surface treatment. Furthermore, the modified surface promoted cell adhesion and proliferation through adjustments in cell morphology. The findings suggest that the combination of femtosecond laser and HA crystals offers a clean, rapid, and controllable approach to preparing bioactive surfaces for zirconia implants. [ABSTRACT FROM AUTHOR]