Search

Abstract Background Fatty acids synthesis and metabolism (FASM)-driven lipid mobilization is essential for energy production during nutrient shortages. However, the molecular characteristics, physiological function and clinical prognosis value of FASM-associated gene signatures in hepatocellular carcinoma (HCC) remain elusive. Methods The Gene Expression Omnibus database (GEO), the Cancer Genome Atlas (TCGA), and International Cancer Genome Consortium (ICGC) database were utilized to acquire transcriptome data and clinical information of HCC patients. The ConsensusClusterPlus was employed for unsupervised clustering. Subsequently, immune cell infiltration, stemness index and therapeutic response among distinct clusters were decoded. The tumor immune dysfunction and exclusion (TIDE) algorithm was utilized to anticipate the response of patients towards immunotherapy, and the genomics of drug sensitivity in cancer (GDSC) tool was employed to predict their response to antineoplastic medications. Least absolute shrinkage and selection operator (LASSO) regression analysis and protein–protein interaction (PPI) network were employed to construct prognostic model and identity hub gene. Single cell RNA sequencing (scRNA-seq) and CellChat were used to analyze cellular interactions. The hub gene of FASM effect on promoting tumor progression was confirmed through a series of functional experiments. Results Twenty-six FASM-related genes showed differential expression in HCC. Based on these FASM-related differential genes, two molecular subtypes were established, including Cluster1 and Cluster2 subtype. Compared with cluster2, Cluster1 subtype exhibited a worse prognosis, higher risk, higher immunosuppressive cells infiltrations, higher immune escape, higher cancer stemness and enhanced treatment-resistant. PPI network identified Acetyl-CoA carboxylase1 (ACACA) as central gene of FASM and predicted a poor prognosis. A strong interaction between cancer stem cells (CSCs) with high expression of ACACA and macrophages through CD74 molecule (CD74) and integrin subunit beta 1 (ITGB1) signaling was identified. Finally, increased ACACA expression was observed in HCC cells and patients, whereas depleted ACACA inhibited the stemness straits and drug resistance of HCC cells. Conclusions This study provides a resource for understanding FASM heterogeneity in HCC. Evaluating the FASM patterns can help predict the prognosis and provide new insights into treatment response in HCC patients.

A Conductor on Round Core (CORC) cable wound with a high-temperature superconductor is an important cable concept for high-current density applications. It is widely used in large power systems because of its advantages of good flexibility and high engineering current density. However, the complex design structure of CORC cable makes it very difficult to understand its electromagnetic properties (such as AC loss). In particular, the different winding directions of each layer in multi-layer cable have a great influence on its electromagnetic characteristics. In this paper, the H-method is used to solve the electromagnetism and mechanics equations. The influence of the winding direction of CORC cable on its electromagnetic field distribution characteristics, AC loss, and mechanical variation under the action of external magnetic field is investigated. The AC loss study of single-layer cable reveals that when the applied magnetic field is increased from 0.01 to 0.02 and 0.03 T, the AC loss peak of the cable increases by 107 and 103 orders of magnitude, respectively, indicating that the effect of low applied magnetic field on the AC loss of the cable is more significant. For multi-layer cables, cables with opposite winding directions have a greater depth of current density penetration than cables with the same winding direction. In addition, the mechanical variations of multi-layer cables with different winding orientations are explored. The results show that the Mises stress in the cable with the same winding direction is about 32% higher than that of the cable with the opposite winding direction, which indicates that the method of winding the cable in the opposite direction between adjacent layers of tape can avoid excessive mechanical stress. [ABSTRACT FROM AUTHOR]