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Porous pure SnO2 nanofibers (NFs) and La2O3 nanoparticles (NPs)-embedded porous SnO2 NFs were successfully synthesized via electrospinning followed by calcination. These materials were systematically evaluated as gas-sensing elements in metal-oxide-semiconductor (MOS) sensors. The La2O3 NPs embedded in porous SnO2 NFs demonstrated superior gas-sensing performance compared to pure SnO2 NFs. Specifically, the incorporation of La2O3 resulted in a 12-fold enhancement in gas-sensing response towards ethanol, significantly improving both sensitivity and selectivity by tuning the carrier concentration and modifying oxygen deficiencies and chemisorbed oxygen levels. Thus, La2O3 NPs embedded in SnO2 NFs present a promising strategy for the development of high-performance ethanol gas sensors. [ABSTRACT FROM AUTHOR]

This work reports on the development of an analyte sampling strategy on a plasmonic substrate to amplify the detection capability of a dual analytical system, paper spray ionization–mass spectrometry (PSI-MS) and surface-enhanced Raman spectroscopy (SERS). While simply applying only an analyte solution to the plasmonic paper results in a limited degree of SERS enhancement, the introduction of plasmonic gold nanoparticles (AuNPs) greatly improves the SERS signals without sacrificing PSI-MS sensitivity. It is initially revealed that the concentration of AuNPs and the type of analytes highly influence the SERS signals and their variations due to the "coffee ring effect" flow mechanism induced during sampling and the degree of the interfacial interactions (e.g., van der Waals, electrostatic, covalent) between the plasmonic substrate and analyte. Subsequent PSI treatment at high voltage conditions further impacts the overall SERS responses, where the signal sensitivity and homogeneity significantly increase throughout the entire substrate, suggesting the ready migration of adsorbed analytes regardless of their interfacial attractive forces. The PSI-induced notable SERS enhancements are presumably associated with creating unique conditions for local aggregation of the AuNPs to induce effective plasmonic couplings and hot spots (i.e., electromagnetic effect) and for repositioning analytes in close proximity to a plasmonic surface to increase polarizability (i.e., chemical effect). The optimized sampling and PSI conditions are also applicable to multi-analyte analysis by SERS and MS, with greatly enhanced detection capability and signal uniformity. [ABSTRACT FROM AUTHOR]

Submerged entry nozzle (SEN) clogging is investigated with an emphasis on the interfacial reaction between the nozzle refractory and the liquid steel. Within the context of carbothermically produced CO(g) being the main cause of the early stage SEN clogging, various SEN refractory materials are assessed in view of their impact on the initial clog deposit formation. A series of high‐temperature experiments are carried out to observe the interfacial reaction between the SEN refractory materials and the liquid steel: three oxide‐based and one oxide‐less refractory materials are employed. Ti‐free and Ti‐added ultra‐low C steel are reacted with these refractory materials. The reactivity of the refractories is assessed by 1) the morphology, composition, and thickness of the initial clog deposit and 2) the evolution of steel compositions. Decreasing SiO2 content in "oxide‐based" refractory is recommended. The "oxide‐less" refractory composed of AlON–BN–AlN is shown to be promising to suppress the early stage of SEN clogging. The present results would provide directions for designing the SEN refractory constitution to suppress the SEN clogging. [ABSTRACT FROM AUTHOR]

Urban green spaces contribute to improving people's physical and mental health by reducing levels of stress and anxiety. Marginalized communities in the USA experience poor health outcome in part because of health disparities related to social and structural determinants of health. The purpose of this study is to assess the prospective role of Kellogg Park, a newly built community park in a marginalized community in California, in enhancing residents' physical and mental health. The researchers collected the data by conducting an in-person survey, which covered questions related to environmental and safety perceptions, overall satisfaction, changes in physical activity changes and mental health with respondents' pre- and post-occupancy experiences. For analyzing data, bivariate analyses using a t-test and a correlation analysis were conducted to examine if there was a relationship between the overall satisfaction and visiting experience. The results indicated an increased positive environmental perception. Besides, respondents reported positively to questions of safety and their overall satisfaction. This research yielded positive results, indicating an improvement in the respondents' physical activity changes and mental health after the construction of the park. The results will advocate for and assist planners, designers, and policy-makers in prioritizing green spaces in marginalized communities. [ABSTRACT FROM AUTHOR]

Rapid, sensitive, and quantitative detection of biomarkers is needed for early diagnosis of disease and surveillance of infectious outbreaks. Here, we exploit a plasmonic syringe filter and surface-enhanced Raman spectroscopy (SERS) in the development of a rapid detection system, using human IgG as a model diagnostic biomarker. The novel assay design facilitates multiple passages of the sample and labeling solution through the detection zone enabling us to investigate and maximize sampling efficiency to the capture substrate. The vertical flow immunoassay process in this study involves the utilization of filter paper embedded with gold nanoparticles (AuNPs) to form a plasmonic substrate. Capture antibody (anti-human IgG) is then immobilized onto the prepared plasmonic paper and inserted into a vertical flow device (syringe filter holder). Sample solution is passed through the filter paper and the target antigen (human IgG) is selectively captured by the immobilized antibody to form an antibody–antigen complex. Next, functionalized AuNPs as extrinsic Raman labels (ERLs) are passed through the filter paper to label the captured biomarker molecules forming a layered structure. This sandwiched geometry enhances plasmonic coupling and SERS signal to provide highly sensitive detection of biomolecules. Systematic studies to investigate the impact of multiple infuse/withdraw cycles of the sample and labeling solutions reveal that antigen and ERL binding are maximized with 10 and 20 cycles, respectively. The optimized assay achieves a detection limit of ∼0.2 ng mL−1 for human IgG with a total assay time of less than 5 minutes, meeting the demands for rapid point of care diagnostics. Additionally, the optimized platform was implemented in the quantitative analysis of the SARS-CoV-2 nucleocapsid protein, the typical target in commercial, FDA-approved rapid antigen tests for COVID-19. [ABSTRACT FROM AUTHOR]

The MRE11 complex (comprising MRE11, RAD50, and NBS1) is integral to the maintenance of genome stability. We previously showed that a hypomorphic Mre11 mutant mouse strain ( Mre11 ATLD1/ATLD1 ) was highly susceptible to oncogene-induced breast cancer. Here we used a mammary organoid system to examine which MRE11-dependent responses are tumor-suppressive. We found that Mre11 ATLD1/ATLD1 organoids exhibited an elevated interferon-stimulated gene (ISG) signature and sustained changes in chromatin accessibility. This Mre11 ATLD1/ATLD1 phenotype depended on DNA binding of a nuclear innate immune sensor, IFI205. Ablation of Ifi205 in Mre11 ATLD1/ATLD1 organoids restored baseline and oncogene-induced chromatin accessibility patterns to those observed in WT. Implantation of Mre11 ATLD1/ATLD1 organoids and activation of the oncogene led to aggressive metastatic breast cancer. This outcome was reversed in implanted Ifi205 -/- Mre11 ATLD1/ATLD1 organoids. These data reveal a connection between innate immune signaling and tumor development in the mammary epithelium. Given the abundance of aberrant DNA structures that arise in the context of genome instability syndromes, the data further suggest that cancer predisposition in those contexts may be partially attributable to chronic innate immune transcriptional programs., (© 2024 Wang et al.; Published by Cold Spring Harbor Laboratory Press.)

Natural and artificial endolysins exhibit bactericidal effects by destroying peptidoglycans in the cell wall of gram-positive bacteria and are usually composed of an N-terminal catalytic domain (CTD) and a C-terminal cell wall-binding domain (CBD). The structures and receptors of CBDs are variable, but bacterial Src homology 3 (SH3b) CBDs are prevalent among the natural endolysins of Staphylococcus aureus. Moreover, although recombinant endolysins with a C-terminal 6-histidine tag (His-tag) are often produced and convenient to purify, the deleterious effects of His-tags on antibacterial activity have not been evaluated thoroughly. Recently, we reported that the antibacterial activity of a commercial lysostaphin without a His-tag differed from that of cell-free lysostaphin with a C-terminal His-tag, and lysostaphin also contains a C-terminal SH3b CBD. In this study, we directly compared the effects of His-tags on the antibacterial activities of lysostaphin and several chimeric lysins possessing different SH3b CBDs. We confirmed that antibacterial activity decreased 16.0-32.0-fold after a His-tag was added to the SH3b CBD.

Nucleolytic resection of DNA ends is critical for homologous recombination, but its mechanism is not fully understood, particularly in mammalian meiosis. Here we examine roles of the conserved MRN complex (MRE11, RAD50, and NBS1) through genome-wide analysis of meiotic resection in mice with various MRN mutations, including several that cause chromosomal instability in humans. Meiotic DSBs form at elevated levels but remain unresected if Mre11 is conditionally deleted, thus MRN is required for both resection initiation and regulation of DSB numbers. Resection lengths are reduced to varying degrees in MRN hypomorphs or if MRE11 nuclease activity is attenuated in a conditional nuclease-dead Mre11 model. These findings unexpectedly establish that MRN is needed for longer-range extension of resection, not just resection initiation. Finally, resection defects are additively worsened by combining MRN and Exo1 mutations, and mice that are unable to initiate resection or have greatly curtailed resection lengths experience catastrophic spermatogenic failure. Our results elucidate multiple functions of MRN in meiotic recombination, uncover unanticipated relationships between short- and long-range resection, and establish the importance of resection for mammalian meiosis.

A stable support substrate for catalytically active metal nanoparticles (NPs) plays an important role in various chemical transformation reactions. This study describes the effective integration of catalytically active palladium nanoparticles (PdNPs) into recycled carbon black (rCB) obtained from scrap tires. The loading efficiency and dispersion degree of PdNPs prepared via a deposition-precipitation method are thoroughly compared to those prepared with conventional Vulcan CB. As the rCB powder exhibits relatively larger surface areas and wider pore size distributions, slightly polydisperse and more PdNPs are integrated across rCB than those on CB. These composite materials are subsequently tested as catalysts in the Suzuki coupling and styrene hydrogenation reactions. For the Suzuki reaction using phenylboronic acid and bromobenzene, the PdNPs on rCB exhibit slightly higher reactivity than those on CB (TOF of ∼9/h vs ∼8/h), presumably due to the structural features of the integrated PdNPs and the relatively hydrophobic characteristics of the rCB substrate. For the hydrogenation reaction, both composite materials easily result in over 99% yield under ambient conditions with similar activation energies of ∼32 kcal/mol. These composite materials are also recyclable in both reactions without a detectable loss of the PdNP catalyst and its activity. Understanding the physicochemical properties of rCB and demonstrating their potential use as a catalyst support substrate evidently suggest the possibility of replacing conventional CB, which also provides an idea of upcycling waste tires in the development of practical and green reaction systems., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)

Here, we describe a SERS-based vertical flow assay as a platform technology suitable for point-of-care (POC) diagnostic testing. A capture substrate is constructed from filter paper embedded with spherical gold nanoparticles (AuNPs) and functionalized with an appropriate capture antibody. The capture substrate is loaded into a filtration device and connected to a syringe to rapidly and repeatedly pass the sample through the sensor for efficient antigen binding. The antigen is then labeled with a SERS-active detection probe. We show that only a few Raman reporter molecules, exclusively located adjacent to the plasmonic capture substrate, generate detectible signals. To maximize the signal from underutilized Raman reporter molecules, we employ a secondary signal enhancing probe that undergoes antibody-directed assembly to form plasmonic core-satellites. This facile enhancement step provides a 3.5-fold increase in the signal and a detection limit of 0.23 ng/mL (1.6 pM) for human IgG. This work highlights the potential to rationally design plasmonic architectures using widely available and reproducible spherical AuNPs to achieve large SERS enhancements for highly sensitive POC diagnostics.