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Aim: The purpose of this study was to evaluate the improvement in acoustic and nasalance in patients with maxillary obturators, using PRAAT software., Materials and Methods: The current study comprised a total of 16 patients who had acquired maxillary defects. Regardless of gender, the age range of these patients was 40-75 years old. The total number of patients were randomly divided into two groups (8 participants in each group) namely (A) Interim obturator group and (B) Definitive obturator group. Analysis was done using PRAAT software for two speech parameters namely nasalance and acoustic value. The first stage of speech analysis was completed without the use of an obturator, whereas the second stage involved inserting the obturator on the same day. The third stage occurred 2 months after the usage of the obturator. The data were recorded and statistically analyzed., Results: In definitive obturator group, mean value for relative nasalance increases from before prosthesis (60.93 ± 3.34 db) to after 2 months of prosthesis (70.53 ± 2.24 db) while in interim group increased from 57.55 ± 4.31 db (before prosthesis) to 63.77 ± 3.66 db (after 2 months of prosthesis). This improvement was more marked with definitive obturator than interim obturator. The F2-F1 comparative mean value changed from before prosthesis to immediate after prosthesis in definitive group for /a/, /e/, and /u/ vowels was 9.77, 22, and 24.38, respectively. F2-F1 comparative mean changes from before prosthesis to after 2 months of prosthesis was 102, 75.75, and 87 for three vowels /a/, /e/, and /u/, respectively. Similarly, F2-F1 mean value in interim prosthesis group changes from before to after 2 months of prosthesis was 62.25, 27.62, and 1.75 for /a/, /e/, and /u/, respectively., Conclusion: On conclusion, the definitive obturators were well-tolerated by patients with major defects and the results show that maxillary resection significantly impairs speech and definitive obturator rehabilitation is effective in regaining nasalance and acoustic speech components., Clinical Significance: Speech disorders in maxillectomy patients are a significant clinical issue since the surgery results in hypernasality, which impairs a person's ability to understand their own speech. Either surgical techniques or prosthetic rehabilitation are used to correct these abnormalities. Certain people require prosthetics to fix their speech even after surgery. How to cite this article: Bhushan P, Thenumkal E, Khosla E, et al. Assessment of Acoustic and Nasalance Improvement in Maxillary Obturator Patients Using PRAAT Software: An In Vivo Study. J Contemp Dent Pract 2024;25(7):656-660.