Search

Background: The most common inherited illness, thalassemia, is thought to have a detrimental effect on public health, particularly in endemic areas. Children with beta thalassemia disease have several mutations. Prevention and premarital examination are still the most effective measures to lessen the burden of beta thalassemia., Objectives: This study primarily aimed to determine the beta thalassemia carriers in relatives of beta thalassemia major children, the role of gene study in the confirmation of beta thalassemia trait diagnosis, and to detect the genetic defect in the relatives of beta thalassemia major children., Materials and Methods: The cross-sectional study was conducted on 109 healthy children, aged between six months and 18 years, who were the relatives (second and fourth degree) of beta thalassemia major cases., Results: Gene screening, using the amplification refractory mutation system (ARMS) polymerase chain reaction (PCR), covered the most common 22 alleles in the Mediterranean region, and was successful in detecting 61.5% of beta-globin chain mutations of studied participants, in addition to high prevalence (34.8%) of beta thalassemia carriers among the relatives of beta thalassemia children., Conclusion: The beta thalassemia carrier rate was found to be highly prevalent among relatives of beta thalassemia major children. Despite the accuracy of gene screening in the detection of beta thalassemia carriers, the use of the most common 22 alleles can only detect 61.5% of carriers; hence, the value of tested gene study is still limited in the detection of carrier rates., Competing Interests: Human subjects: Consent was obtained or waived by all participants in this study. Ethics Committee of the Faculty of Medicine, Assiut University issued approval 04-2023-10006. Written informed consents were taken from parents with an explanation of the benefits of the study, risks expected, and suggested treatment for each case. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Elasheer et al.)

Introduction: Streptococcusmutans and lactobacilli are most important bacteria in the pathogenesis of dental caries. Cariogenic microflora has been associated to the primary caregiver transmission and sugary diets., (This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Aim: Resin-modified glass ionomer cement tends to shrink due to polymerization of the resin component. Additionally, they are more prone to syneresis and imbibition during the setting process. This in vitro study evaluates the impact of chitosan, a biopolymer that is, both biomaterial and biocompatible, on the strength of dentin bonding and compares it with ACTIVA Bio-ACTIVE Restorative. The present study was aimed to assess the impact of including chitosan into Fuji II on the shear bond strength between. the restoration material and tooth dentin, in contrast to Bioactiva in permanent teeth., Materials and Methods: A total of 30 premolar teeth were recently extracted. The study involved three distinct sample groups. Group 1 (10 teeth) is the negative control (Fuji II), Group 2 (10 teeth) is the positive control (ACTIVA Bio-ACTIVE Restorative), and Group 3 (10 teeth) is treated with a mixture of Chitosan and Fuji II (CH-Fuji II). Each tooth's buccal and palatal cusps were eliminated to achieve a horizontal surface. Using a periodontal probe, 1.5 mm from the mesial pit to the mesial marginal ridge were removed. Restoration was implemented in all groups following manufacturer directions. Thermocycling the teeth by immersing them in a water bath with temperatures ranging from 5°C to 55°C (± 1-2°C) for 30 s (500 cycles). Each sample was attached to the universal testing machine's jig at a cross-head speed of 1 mm/min. Shear force was used until breakage, and the bond's adhesive strength was then calculated. Statistical analysis using ANOVA with Dunnett's T3 post hoc test. Results were significant at P < 0.05., Results: Statistically significant difference was present between Chitosan and Fuji II and between Chitosan and Activa by reducing the shear bond strength., Conclusions: Addition of chitosan to Fuji II had a negative effect on the shear bond with a significant difference while Activa and Fuji II exhibited favorable shear bond strength., Competing Interests: There are no conflicts of interest to disclose., (Copyright: © 2024 Journal of International Society of Preventive and Community Dentistry.)