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A facultative anaerobic, Gram-stain-negative, non-motile, rod-shaped bacterial strain AGMB14963 T was isolated from the feces of a dairy cow. A 16S rRNA gene sequence-based phylogenetic analysis revealed that strain AGMB14963 T belongs to the genus Gallibacterium, with Gallibacterium salpingitidis F150 T being the closest species (95.8% 16S rRNA gene sequence similarity). Whole-genome sequence analysis revealed that strain AGMB14963 T had a 37.0% G + C genomic DNA content and a genome size of 2.58 Mb. In addition, the strain contained 53 tRNA and 2 rRNA genes. The average nucleotide identity and digital DNA-DNA hybridization values between strains AGMB14963 T and G. salpingitidis F150 T were 82.3 and 24.9%, respectively. Further, strain AGMB14963 T was positive for oxidase and catalase, but negative for urease. Optimal growth of strain AGMB14963 T occurred at 37 ℃, pH 8, and in 0.5% NaCl-containing medium. The predominant cellular fatty acids (> 10%) in strain AGMB14963 T were C 14:0 , C 16:0 , and summed feature 3. Strain AGMB14963 T produced lactic acid and isobutyric acid as the major end products of glucose fermentation. Polar lipids consisted of one phospholipid and one phosphoaminolipid. According to the genomic, physiologic, and chemotaxonomic characteristics, strain AGMB14963 T represents a novel species of the genus Gallibacterium, for which the name Gallibacterium faecale sp. nov. is proposed. The type strain is AGMB14963 T (= KCTC 25487  T  = NBRC 116419  T )., Competing Interests: Declarations. Conflicts of interest: The authors declare that there are no conflicts of interest., (© 2025. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)