1. tRNA‐derived fragments are altered in diabetes.
- Author
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Ng, N., Gibriel, H. A. Y., Halang, L., Jirström, E., Ioana, J. A., Burke, M., Byrne, M. M., and Prehn, J. H. M.
- Subjects
GENETICS of diabetes ,DIAGNOSIS of diabetes ,GENETICS of deafness ,BIOMARKERS ,REVERSE transcriptase polymerase chain reaction ,MATURITY onset diabetes of the young ,ALBUMINS ,STATISTICS ,GLYCOSYLATED hemoglobin ,TRANSFER RNA ,SEQUENCE analysis ,ACADEMIC medical centers ,HIGH performance liquid chromatography ,SERUM ,ONE-way analysis of variance ,TYPE 1 diabetes ,CASE-control method ,MICRORNA ,BLOOD collection ,MANN Whitney U Test ,REGRESSION analysis ,BLOOD sugar ,TYPE 2 diabetes ,GENE expression ,IMMUNOASSAY ,COMPARATIVE studies ,RESEARCH funding ,FLUORESCENT antibody technique ,DESCRIPTIVE statistics ,URINALYSIS ,DATA analysis software ,DATA analysis ,RECEIVER operating characteristic curves ,SENSITIVITY & specificity (Statistics) ,PHENOTYPES ,CREATININE ,C-peptide - Abstract
Aims: Maternally inherited diabetes and deafness (MIDD) is a rare form of adult‐onset diabetes that can be difficult to diagnose due to its variable clinical phenotype. Transfer RNA‐derived small fragments are a novel, emerging class of small non‐coding RNAs (sncRNAs) that have significant potential as serum biomarkers due to their stress‐induced generation, abundance, stability and ease of detection. Methods: We investigated the levels of tiRNA 5'ValCAC (alone and in combination with miR‐23b‐3p) identified from small RNA sequencing studies in serum samples from healthy controls, type 1 diabetes, type 2 diabetes and MIDD subjects. Results: Serum levels of 5'ValCAC were reduced in MIDD and type 2 diabetes subjects compared to controls. Type 2 diabetes subjects had higher serum levels of miR‐23b‐3p compared to all other subjects. Receiver Operating Characteristic analysis showed the potential of 5'ValCAC and miR‐23b‐3p as MIDD biomarkers, with the combination showing excellent separation from type 2 diabetes subjects. Conclusions: This is the first report showing altered serum levels of tiRNAs in diabetes subjects. The combined use of 5'ValCAC and miR‐23b‐3p as serum biomarkers could potentially differentiate between MIDD subjects and type 2 diabetes subjects. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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