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The present study aimed to evaluate the histologic, histochemical, and immunohistochemical changes in buffalo livers with cystic echinococcosis. Noninfected and infected livers were collected from the freshly slaughtered buffalo at the Aligarh abattoir. Small pieces of both infected and noninfected livers ( n = 5) were cut and processed for histologic and histochemical studies. Immunohistochemistry was performed using rabbit anti-CD3, CD19, and CD117 antibodies. The results revealed the presence of brood capsules and germinal and laminated membranes surrounded by a fibrous adventitial layer, followed by moderate and diffused infiltration of eosinophils, monocytes, neutrophils, lymphocytes, and marked focal infiltration of mast cells. The infected livers also had mild dilation of central veins and sinusoids, mild and focal necrosis of hepatic tissue, and congestion of central and portal veins. Periodic acid-Schiff reaction revealed marked glycogen depletion in the infected liver. Masson's trichrome stain showed marked deposition of collagen fibers in the portal area, adventitial layer, and between the hepatocytes compared with the noninfected liver, where deposition was found only in the portal area. The T-cell response was more pronounced than the B-cell response in infected liver. Thus, it can be concluded that hydatid cyst infection causes several pathological and biochemical changes and increased infiltration of inflammatory cells in the infected livers, suggesting the involvement of nonspecific immune responses against hydatid cysts. The T-cell response was more pronounced than B-cells, indicating the involvement of cell-mediated immunity against cystic echinococcosis. These findings may help to understand the local immune responses to cystic echinococcosis., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.