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Your search keyword '"INTESTINAL TROPISM"' showing total 3 results
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3 results on '"INTESTINAL TROPISM"'

1. Rapid Generation of Reverse Genetics Systems for Coronavirus Research and High-Throughput Antiviral Screening Using Gibson DNA Assembly.

Author

Zhou X, Liu H, Yang S, Dong X, Xie C, Ou W, Chen J, Yang Z, Ye Y, Ivanov KI, Liu L, Zou J, Li C, and Guo D

Subjects
Humans, Animals, Coronavirus genetics, Coronavirus drug effects, Mice, Drug Evaluation, Preclinical methods, SARS-CoV-2 genetics, SARS-CoV-2 drug effects, Genome, Viral genetics, Murine hepatitis virus genetics, Murine hepatitis virus drug effects, DNA, Complementary genetics, Coronavirus 229E, Human genetics, Coronavirus 229E, Human drug effects, Cell Line, Reverse Genetics methods, Antiviral Agents pharmacology, Chromosomes, Artificial, Bacterial genetics, High-Throughput Screening Assays methods
Abstract

Coronaviruses (CoVs) pose a significant threat to human health, as demonstrated by the COVID-19 pandemic. The large size of the CoV genome (around 30 kb) represents a major obstacle to the development of reverse genetics systems, which are invaluable for basic research and antiviral drug screening. In this study, we established a rapid and convenient method for generating reverse genetic systems for various CoVs using a bacterial artificial chromosome (BAC) vector and Gibson DNA assembly. Using this system, we constructed infectious cDNA clones of coronaviruses from three genera: human coronavirus 229E (HCoV-229E) of the genus Alphacoronavirus, mouse hepatitis virus A59 (MHV-59) of Betacoronavirus, and porcine deltacoronavirus (PDCoV-Haiti) of Deltacoronavirus. Since beta coronaviruses including severe acute respiratory syndrome coronavirus (SARS-CoV), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and Middle East respiratory syndrome coronavirus (MERS-CoV) represent major human pathogens, we modified the infectious clone of the beta coronavirus MHV-A59 by replacing its NS5a gene with a fluorescent reporter gene to create a system suitable for high-throughput drug screening. Thus, this study provides a practical and cost-effective approach to developing reverse genetics platforms for CoV research and antiviral drug screening., (© 2025 Wiley Periodicals LLC.)

Published
2025
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3. The Cat - E-Book : The Cat - E-Book

Author

Susan E. Little and Susan E. Little

Subjects
Cats--Nutrition, Cats--Behavior, Cats--Diseases--Diagnosis, Cats--Diseases--Treatment
Abstract

Comprehensive in scope and exclusively devoted to feline medical care, Dr. Susan Little's The Cat: Clinical Medicine and Management, 2nd Edition, is an essential resource for providing complete, state-of-the-art care to cats. Authoritative, clinically focused information is enhanced by full-color images, tables, boxes, algorithms, key points, and more — all in an easy-to-understand, quick-reference format. Dr. Little and her expert contributors address the unique concerns and challenges facing any practitioner providing care for cats, including the latest advances in feline medical diagnosis and management and the clinical applications to everyday practice. An eBook version, included with print purchase, provides access to all the text, figures, and references, with the ability to search, customize content, make notes and highlights, and have content read aloud. The eBook also offers convenient video lessons and client handouts. - Covers the latest advances in feline medicine from a systemic and adjunctive care perspective, making it the most comprehensive feline medical reference available with a strong clinical focus. - Addresses key topics unique to feline medicine and not currently covered in other books, including: insights and clinical advances attributable to the mapping of the feline genome; medical conditions associated with behavioral problems; managing the feline patient with co-existing and chronic diseases; special medical problems and care considerations for the geriatric cat; environmental enrichment for the indoor cat; feline zoonotic agents and implications for human health; and humane solutions for shelter medicine and overpopulation challenges. - Helps meet the increasing demand for state-of-the-art medical care by cat owners, including advanced diagnostic services and treatments designed to extend and improve quality of life for feline companions. - Features a full-color design with hundreds of schematic drawings, tables, boxes, key points, algorithms, and photographs for quick and easy access to information. - Provides in-depth information on indoor cats and senior cats, including timely guidance on meeting owners'expectations for longer, healthier lives for their cats. - Addresses the challenges of cat overpopulation, particularly humane approaches to improve quality of life for millions of feral and community cats and minimize their impact on public health and endangered species of animals. - Presents information written in the manner of expanded conference proceedings, delivering the latest insights and most current approaches to management of feline medical disorders. - Includes contributions from approximately 80 contributors, including many from outside the US, to provide a global perspective and valuable expertise from those most knowledgeable in the field of feline medical care.

Published
2025

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