1. Exploring fluoropolymers for fabrication of femtoliter chamber arrays used in digital bioanalysis.
- Author
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Ando J, Murai K, Mori M, Michiyuki T, Iida T, Makino A, Shinoda H, and Watanabe R
- Subjects
- Horseradish Peroxidase metabolism, Horseradish Peroxidase chemistry, beta-Galactosidase metabolism, Polymers chemistry
- Abstract
The global supply of fluoropolymers and fluorinated solvents is decreasing due to environmental concerns regarding polyfluoroalkyl substances. CYTOP has been used for decades primarily as a component of a femtoliter chamber array for digital bioanalysis; however, its supply has recently become scarce, increasing the urgency of fabricating a femtoliter chamber array using alternative materials. In this study, we investigated the feasibility of fabricating a femtoliter chamber array using four types of fluoropolymers in stable supply as candidate substitutes and verified their applicability for digital bioanalysis. Among these candidates, Fluorine Sealant emerged as a viable option for fabricating femtoliter chamber arrays using a conventional photolithography process. To validate its efficacy, we performed various digital bioanalysis using FP-A-based chamber arrays with model enzymes such as CRISPR-Cas, horseradish peroxidase, and β-galactosidase. The results demonstrated the similar performance to that of CYTOP, highlighting the broader utility of FP-A in digital bioanalysis. Our findings underscore the potential of FP-A to enhance the versatility of digital bioanalysis and foster the ongoing advancement of innovative diagnostic technologies., (© 2024. The Author(s).)
- Published
- 2024
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