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Escherichia coli is an important antibiotic-resistant pathogen in mastitis, with broader public health implications. We report the genomes of two E. coli strains, MBBL4 and MBBL5, isolated from mastitic cow milk. The draft genomes, covering 4.6 Mbp each, revealed 57.5% GC content and 100× sequencing depth.

Milk from cows with mastitis is a primary source of bacteria harboring antibiotic resistance genes (ARGs), including Klebsiella pneumoniae . We present the genome sequence of K. pneumoniae strain MBBL2 isolated from mastitic cow milk, which contains numerous ARGs and virulence-associated genes potentially pathogenic to humans.

We sequenced the whole genome of Enterococcus faecium MBBL3, isolated from healthy cow milk. The draft genome of MBBL3 is 2,971,195 bp with 95.61× coverage, spanned over 106 contigs., Competing Interests: The authors declare no conflict of interest.