Search

AbstractBackgroundObjectivesMaterial and methodsResultsConclusions and significanceThe Canalith Repositioning Maneuver (CRM) is often ineffective for persistent geotropic direction-changing positional nystagmus (DCPN). In these cases, determining the lesion side can be challenging, as the null plane side and the side with stronger nystagmus on the roll test are frequently opposite.This study investigates whether the low therapeutic efficacy of CRM in persistent DCPN could be attributed to incorrect determination of the lesion side.This prospective randomized trial involved two groups of patients with persistent geotropic DCPN, categorized based on the method used to determine the lesion side: the null plane side and the side with stronger nystagmus on the head roll test. We assessed the therapeutic efficacy of CRM on the null plane side (null plane group) and the side with stronger nystagmus on the head roll test (stronger nystagmus group).Both groups showed minimal or no immediate therapeutic effect of CRM, with a low short-term effect observed in both as well. No significant differences were found in the immediate and short-term therapeutic effects of CRM between the two groups.The limited therapeutic effect of CRM in persistent geotropic DCPN does not appear to result from incorrect determination of the lesion side. [ABSTRACT FROM AUTHOR]

Background: Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality worldwide. Single-cell RNA sequencing (scRNA-seq) provides gene expression profiles at the single-cell level. Hence, we evaluated gene expression in the peripheral blood of patients with COPD. Methods: Peripheral blood samples from seven healthy controls and eight patients with COPD were obtained in this study. The 10X Genomics Chromium Instrument and cDNA synthesis kit were utilized to generate a barcoded cDNA library for single cell RNA-sequencing. We compared the scRNA-seq data between the COPD and control groups using computational analysis. Functional analyses were performed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. Results: scRNA-seq was used to analyze the transcriptome of peripheral blood mononuclear cells from seven normal controls and eight patients with COPD. We found an increased number of monocyte/macrophages in the COPD group compared to the normal control group. Among the differentially expressed genes (DEGs) in monocyte/macrophages, we identified 15 upregulated genes (EGR1, NR4A1, CCL3, CXCL8, PTGS2, CD83, BCL2A1, SGK1, IL1B, BTG2, NFKBIZ, DUSP2, MAFB, PLAUR and CCL3L1) and 7 downregulated genes (FOLR3, RPS4Y1, HLA-DRB5, NAMPT, CD52, TMEM176A and TMEM176B) in the COPD group compared to the normal control group. Conclusions: Using scRNA-seq, we found differences in cell type distribution, especially in monocyte/ macrophages. Several upregulated and downregulated genes were found in the monocyte/macrophages of the COPD group. [ABSTRACT FROM AUTHOR]