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Your search keyword '"Holčapek, Michal"' showing total 8 results
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8 results on '"Holčapek, Michal"'

3. The lipidomics reporting checklist a framework for transparency of lipidomic experiments and repurposing resource data

Author

Kopczynski, Dominik, Ejsing, Christer S., McDonald, Jeffrey G., Bamba, Takeshi, Baker, Erin S., Bertrand-Michel, Justine, Brügger, Britta, Coman, Cristina, Ellis, Shane R., Garrett, Timothy J., Griffiths, William J., Guan, Xue Li, Han, Xianlin, Höring, Marcus, Holčapek, Michal, Hoffmann, Nils, Huynh, Kevin, Lehmann, Rainer, Jones, Jace W., Kaddurah-Daouk, Rima, Köfeler, Harald C., Meikle, Peter J., Metz, Thomas O., O’Donnell, Valerie B., Saigusa, Daisuke, Schwudke, Dominik, Shevchenko, Andrej, Torta, Federico, Vizcaíno, Juan Antonio, Welti, Ruth, Wenk, Markus R., Wolrab, Denise, Xia, Yu, Ekroos, Kim, Ahrends, Robert, and Liebisch, Gerhard

Published
2024
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8. Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards.

Author

Torta F, Hoffmann N, Burla B, Alecu I, Arita M, Bamba T, Bennett SAL, Bertrand-Michel J, Brügger B, Cala MP, Camacho-Muñoz D, Checa A, Chen M, Chocholoušková M, Cinel M, Chu-Van E, Colsch B, Coman C, Connell L, Sousa BC, Dickens AM, Fedorova M, Eiríksson FF, Gallart-Ayala H, Ghorasaini M, Giera M, Guan XL, Haid M, Hankemeier T, Harms A, Höring M, Holčapek M, Hornemann T, Hu C, Hülsmeier AJ, Huynh K, Jones CM, Ivanisevic J, Izumi Y, Köfeler HC, Lam SM, Lange M, Lee JC, Liebisch G, Lippa K, Lopez-Clavijo AF, Manzi M, Martinefski MR, Math RGH, Mayor S, Meikle PJ, Monge ME, Moon MH, Muralidharan S, Nicolaou A, Nguyen-Tran T, O'Donnell VB, Orešič M, Ramanathan A, Riols F, Saigusa D, Schock TB, Schwartz-Zimmermann H, Shui G, Singh M, Takahashi M, Thorsteinsdóttir M, Tomiyasu N, Tournadre A, Tsugawa H, Tyrrell VJ, van der Gugten G, Wakelam MO, Wheelock CE, Wolrab D, Xu G, Xu T, Bowden JA, Ekroos K, Ahrends R, and Wenk MR

Subjects
Humans, Calibration, Mass Spectrometry methods, Lipidomics methods, Reproducibility of Results, Ceramides blood, Reference Standards, Laboratories standards
Abstract

In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method and/or their method of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. These results are the most precise (intra-laboratory CVs ≤ 4.2%) and concordant (inter-laboratory CVs < 14%) community-derived absolute concentration values reported to date for four clinically used ceramides in the commonly analyzed SRM 1950. We demonstrate that calibration using authentic labelled standards dramatically reduces data variability. Furthermore, we show how the use of shared RM can correct systematic quantitative biases and help in harmonizing lipidomics. Collectively, the results from the present study provide a significant knowledge base for translation of lipidomic technologies to future clinical applications that might require the determination of reference intervals (RIs) in various human populations or might need to estimate reference change values (RCV), when analytical variability is a key factor for recall during multiple testing of individuals., (© 2024. The Author(s).)

Published
2024
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