Search

Sequestration of Plasmodium parasites in the placental vasculature causes increased morbidity and mortality in pregnant compared to non-pregnant patients in malaria- endemic regions. In this study, outbred pregnant CD1 mice with semi allogeneic fetuses were infected with transgenic Plasmodium berghei or mock-inoculated by mosquito bite at either embryonic day (E) 6 (first trimester-equivalent) or 10 (second trimester- equivalent) and compared with non-pregnant females. P. berghei -infected mosquitoes had greater biting avidity for E10 dams than uninfected mosquitoes, which was not apparent for E6 dams nor non-pregnant females. Infected E10 dams had greater numbers of parasites than E6 dams in the uterus and spleen, but not in the blood or liver. While parasites were found in placentas, no parasites were present in fetuses. Maternal infection at E6 caused greater maternal morbidity, with greater rates of fetal reabsorption and stillbirths than at E10. Infection at E10 caused adverse offspring outcomes, including growth restriction. To identify possible mechanisms of adverse offspring outcomes, E10 dams were euthanized during peak parasitemia (8 days post infection), and outcomes were compared with mock-infected dams. P. berghei caused significant systemic maternal immune activation with elevated circulating lymphocytes, eosinophils, and neutrophils and splenic cytokine concentrations. P. berghei infection at E10 increased corticosterone and decreased progesterone concentrations, which could contribute to adverse perinatal outcomes through immunomodulation. There were limited changes in the maternal fecal microbiome after P. berghei infection. Mosquito bite infection of outbred dams with P. berghei causes placental malaria and provides a novel, tractable model to investigate therapeutic treatments.

Bullying is a persistent social and behavioral problem in the United States. Bullying victimization and perpetration are linked to a host of negative physical, social, and emotional outcomes. Research suggests that a key risk factor for bullying behaviors is adverse childhood experiences (ACEs). On the other hand, positive childhood experiences (PCEs) may counter some of the negative effects of ACEs. This study (a) assesses the independent effects of ACEs and PCEs on adolescent bullying victimization and perpetration, and (b) examines whether ACEs and PCEs interact to affect bullying victimization and perpetration.We use data from the 2020 to 2021 National Survey of Children's Health, a nationally representative survey of children ages 0 to 17 in the United States. The analytic sample was limited to children who were 6 years old or older at the time of the survey ( N  = 60,809). Using caregiver reports of bullying victimization and perpetration, we created a cumulative ACEs scale comprised of 10 items and a cumulative PCEs scale comprised of eight measures. We then estimated a set of logistic regression models to predict bullying behaviors. The results showed that ACEs are associated with a higher likelihood of both bullying victimization and perpetration. Although PCEs have a slight mitigating effect, ACEs and PCEs interact such that even in the presence of PCEs, children with many ACEs still have a higher likelihood of both bullying victimization and perpetration. This highlights the considerable impact of ACEs on bullying behaviors. These findings suggest that enhancing ACE-aware care and ACE prevention is important because even promoting PCEs is unlikely to decrease bullying levels on their own., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interests with respect to the authorship and/or publication of this article.