9 results on '"Henn, Dominic"'
Search Results
2. Avenanthramide and β-Glucan Therapeutics Accelerate Wound Healing via Distinct and Nonoverlapping Mechanisms
- Author
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Kussie, Hudson, primary, Hahn, William W, additional, Sivaraj, Dharshan, additional, Quintero, Filiberto, additional, Knochel, Amelia Belle, additional, Alsharif, Abdelrahman Marwan, additional, Yasmeh, Jonathan Parham, additional, Fischer, Katharina, additional, Mojadidi, Sultana, additional, Hostler, Andrew, additional, Granoski, Maia B, additional, Mckenna, Eamonn, additional, Henn, Dominic, additional, Litmanovich, Ben, additional, Miller, Abigail, additional, Schurr, Delaney, additional, Li, Vincent W, additional, Li, William, additional, Gurtner, Geoffrey C, additional, and Chen, Kellen, additional
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- 2024
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3. Aesthetic Implications of Bilateral Profunda Artery Perforator Flaps on Thigh and Buttock Proportions.
- Author
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Henn, Dominic, Lakatta, Alexis, Teotia, Sumeet S., and Haddock, Nicholas T.
- Subjects
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PERFORATOR flaps (Surgery) , *MAMMAPLASTY , *PATIENTS' attitudes , *THIGH , *BUTTOCKS , *BODY mass index - Abstract
Background The profunda artery perforator (PAP) flap has emerged as an excellent secondary option for autologous breast reconstruction. Despite the increased acceptance, potential secondary benefits concerning aesthetic proportions of proximal thigh and buttock at the donor site have never been systematically investigated. Methods A retrospective review of 151 patients who underwent breast reconstruction with horizontally designed PAP flaps (292 flaps) from 2012 to 2020 was performed. Patient characteristics, complications, and numbers of revision surgeries were collected. In bilateral reconstructions, pre- and postoperative standardized patient photographs were analyzed to identify postoperative changes in proximal thigh and buttock contour. The patients' own perception of postoperative aesthetic changes was determined by an electronic survey. Results The patients had a mean age of 51 and a mean body mass index of 26.3 kg/m 2. The most common complications were minor and major wound complications affecting 35.1% of patients, followed by cellulitis (12.6%), seroma (7.9%), and hematoma (4.0%). A total of 38 patients (25.2%) underwent revision of the donor site. After reconstruction, patients were found to have aesthetically improved proximal thigh and buttock proportions, indicated by a wider thigh gap (thigh gap-hip ratio: 0.05 ± 0.04 vs. 0.13 ± 0.05, p < 0.0001) and reduction in lateral thigh-to-buttock ratio (0.85 ± 0.05 vs. 0.76 ± 0.05, p < 0.0001). Among the 85 patients who responded to the survey (56.3% response rate), 70.6% felt that PAP surgery had aesthetically either improved their thigh contour (54.12%) or not changed it (16.47%), whereas only 29.4% reported that the surgery negatively impacted their thigh contour. Conclusion PAP flap breast reconstruction leads to improved aesthetic proportions of the proximal thigh and buttock. This approach is ideal for patients with ptotic tissue of the inferior buttocks and medial thigh, a poorly defined infragluteal fold, and inadequate anterior-posterior buttock projection. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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4. Breast Reconstruction With Simultaneous Bilateral Lumbar Artery Perforator Flaps Improves Waistline Definition and Buttock Projection.
- Author
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Teotia, Sumeet S, Henn, Dominic, Lakatta, Alexis, Steppe, Cyrus, and Haddock, Nicholas T
- Abstract
Background The lumbar artery perforator (LAP) flap is a valuable secondary option for autologous breast reconstruction when abdominal donor sites are not available. Objectives The aim of this study was to determine how the LAP donor site affects waistline and gluteal proportions, and whether LAP flaps may produce potential secondary benefits concerning donor site aesthetics. Methods A retrospective review of 50 patients who underwent bilateral breast reconstruction with LAP flaps (100 flaps) between 2018 and 2022 was performed. Patient characteristics and postoperative complications were recorded. Pre- and postoperative standardized frontal and lateral patient photographs were analyzed to identify postoperative changes in waist-to-hip ratio (WHR) to assess waistline definition and buttock projection. The patients' individual perception of postoperative aesthetic changes to their waistline and buttocks was determined by an electronic survey. Results The patients had a mean age of 51 years (range, 24-63 years) and a mean BMI of 26.9 kg/m
2 (range, 19.3-37.4 kg/m2 ). Postoperative donor site complications included seroma (n = 10), wound dehiscence (n = 5), hematoma (n = 4), and wound infection (n = 2). Flap loss rate was 2%. After reconstruction, patients were found to have a more defined waistline, indicated by a significantly decreased WHR on frontal images (mean [standard error of the mean], 0.85 [0.05] vs 0.80 [0.05], P <.005) and a more projected buttocks indicated by a significant reduction in WHR on lateral images (0.92 [0.07] vs 0.87 [0.07], P <.0001). Among the patients who responded to the survey, 73% indicated that their waistline had aesthetically improved, 6.7% felt it had not changed, and 20% felt that it had worsened. An aesthetic improvement of the buttocks was reported by 53%, 40% felt their buttocks had not changed, and 6.7% felt their buttocks appearance had worsened. Conclusions Bilateral LAP flap breast reconstruction leads to improved waistline definition and buttock projection, bringing patients closer to ideal aesthetic proportions. This reconstructive approach is ideal for patients who are not candidates for abdominal free flaps, but who demonstrate excess flank tissue and seek a more defined waistline and projected buttocks. Level of Evidence: 4 [ABSTRACT FROM AUTHOR]- Published
- 2024
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5. 31 - Implants and biomaterials
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Sivaraj, Dharshan, Henn, Dominic, King, Timothy W., and Chen, Kellen
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- 2024
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6. List of Contributors
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Abou-Sayed, Hatem, Afrooz, Paul N., Al Deek, Nidal F., Berli, Jens U., Boyd, Kirsty Usher, Brix, Eva, Brown, Stav, Broyles, Justin M., Byrd, Jacqueline N., Cai, Lawrence, Cao, Yilin, Chen, Kellen, Ch'ng, Sydney, Chung, Kevin C., Cladis, Franklyn P., Clark, Audra, Clarke, Alex, Coriddi, Michelle, Diehm, Yannick F., Erdmann-Sager, Jessica, Fahy, Evan, Gallo, Lucas, Gosman, Amanda, Gregorowitsch, Madelijn, Griffin, Michelle F., Gurtner, Geoffrey C., Harms, Karl-Anton, Haug, Valentin, Helliwell, Lydia, Hendren-Santiago, Bryce, Henn, Dominic, Ho, George, Hong, Joon Pio, Hu, Michael S., Hultman, C. Scott, Jazayeri, Leila, Jeffer, Haley M., Jeffers, Lynn, Kane, Gabrielle M., Kauke-Navarro, Martin, King, Timothy W., Klassen, Anne F., Kuehlmann, Britta A., Kuzon, William M., Jr, Levi, Benjamin, Liu, Daniel Z., Liu, Wei, Loder, Shawn, Longaker, Michael T., Peter Lorenz, H., Mackinnon, Susan E., Manahan, Michele A., Mazzola, Isabella C., Mazzola, Riccardo F., Mehrara, Babak J., Momeni, Arash, Morris, Steven F., Morrison, Shane D., Neligan, Peter C., Nelson, Jonas A., Nthumba, Peter, Nuutila, Kristo, Offodile, Anaeze C., Ogawa, Rei, Pannucci, Christopher J., Perrault, David, Pomahac, Bohdan, Power, Hollie A., Prantl, Lukas M., Preminger, B. Aviva, Pripotnev, Stahs, Pusic, Andrea L., Reid, Russell R., Riesel, Johanna N., Rubin, J. Peter, Rumsey, Nichola, Sarhane, Karim A., Schaub, Stephanie K., Seitz, Iris A., Selber, Jesse C., Shayan, Ramin, Sheckter, Clifford C., Sinha, Indranil, Lin Tay, Sherilyn Keng, Taylor, G. Ian, Teven, Chad M., Thoma, Achilleas, Thorne, Charles H., Tsai, Joseph, Varey, Alexander H.R., Varon, David E., Voineskos, Sophocles H., Wei, Fu-Chan, Wilson, Stelios C., Young-Afat, Danny, Zhou, Guangdong, Zielins, Elizabeth R., Almukhtar, Rawaa, A. Alsubaie, Saad, Alter, Gary J., Aly, Al S., Amalfi, Ashley N., Anderson, Eric W., Armijo, Bryan, Aschen, Seth Z., Baker, Daniel C., Barrera, Alfonso, Bellamy, Justin, Bensimon, Richard Hector, Berry, Miles G., Bruner, Terrence W., Cánchica Cano, Andrés F., Capella, Joseph Francis, Centeno, Robert F., Coleman, Sydney R., Constantian, Mark B., Cook, Jonathan, Choi, Hong Lim, Choi, Jong Woo, Coleman, Jayne, Coon, Devin, Davies, Dai M., Salcedo, Jose Abel De la Pena, Del Vecchio, Daniel A., Draelos, Zoe Diana, Di Bernardo, Barry, Eaves, Felmont F., Egro, Francseco M., Elyassnia, Dino, Ellis, Marco, Fabi, Sabrina G., Few, Julius, Jr, Flugstad, Nicholas A., Frame, James D., Frojo, Gianfranco, Gonzalez, Jazmina M., Sosa, David Gonzalez, Grow, Jacob, Gruber, Ronald P., Gusenoff, Jeffrey, Guyuron, Bahman, Hadeed, Josef G., Hunstad, Joseph, Ishii, Clyde, Janis, Jeffrey E., Joseph, Jeremy T., Kahn, David M., Keller, Patrick R., Kenkel, Jeff, Rodriguez, Jocelyn Celeste Ledezma, Levine, Steven, Locke, Michelle, Louis, Matthew, Luthringer, Margaret, Maliha, Samantha G., Mariwalla, Kavita, Marten, Timothy, Matarasso, Alan, Mendelson, Bryan Christopher, Mendieta, Constantino G., Miotto, Gabriele C., Nahai, Foad, Oh, Tae Suk, Paiva, Sabina, Paul, Malcolm, Perdikis, Galen, Pozner, Jason, Ramanadham, Smita R., Richter, Dirk F., Rochlin, Danielle H., Roberts, Thomas L., Rohrich, Rod J., Rubin, Peter J., Rullan, Peter P., Saldanha, Cristianna Bonetto, Saldanha, Osvaldo Ribeiro, Filho, Osvaldo Saldanha, Saltz, Renato, Schoenbrunner, Anna, Schwaiger, Nina, Shulzhenko, Nikita O., Singh, Amitabh, Spinelli, Henry M., Stuzin, James M., Szolomicki, Taisa, Toledo, Luiz, Tonnard, Patrick, Totonchi, Ali, Toy, Jonthan W., Tzur, Rotem, Turer, David, Verpaele, Alexis, Wall, Simeon, Jr, Warren, Richard J., Wong, Chin-Ho, Zhu, Victor, Zide, Barry M., Zins, James E., Adler, Neta, Alabdulkarim, Abdulaziz, Alperovich, Michael, Alvarado, Marta, Antonyshyn, Oleh M., Arnaud, Eric, Aronson, Sofia, Baker, Stephen B., Balkin, Daniel M., Bartlett, Scott P., Bauer, Bruce S., Baylis, Adriane L., Beederman, Maureen, Beh, Han Zhuang, Bentz, Michael, Bergman, Hannah J., Berman, Zoe P., Billig, Allan B., Birgfeld, Craig B., Borschel, Gregory H., Boyd, John Brian, Bradley, James P., Buchanan, Edward P., Buchman, Steven R., Buller, Mitchell, Bykowski, Michael R., Capitán, Luis, Capitán-Cañadas, Fermín, Carlson, Anna R., L. Chang, Brian, Chen, Philip Kuo-Ting, Chen, Yu-Ray, Cheng, Ming-Huei, Chinchilla, Gerson R., Cho, Min-Jeong, Cordeiro, Peter G., Cugno, Sabrina, Daeschler, Simeon C., Dempsey, Robert F., Dibbs, Rami P., Dickie, Sara R., Do, Nicholas, Ettinger, Russell E., Ferry, Andrew M., Figueroa, Alexander L., Figueroa, Alvaro A., Fisher, David M., Flores, Roberto L., Forrest, Christopher R., Gilardino, Mirko S., Glaser, Daniel H., Goldstein, Jesse A., Gosain, Arun K., Gottlieb, Lawrence J., Greene, Arin K., Greives, Matthew R., Haber, Samer E., Halsey, Jordan N., Hammoudeh, Jeffrey, Hanasono, Matthew M., Helms, Jill A., Heuer, Gregory G., Hirsch, David L., Hollier, Larry H., Jr, Hopper, Richard A., Jacobson, Adam S., James, Syril, Jimenez, Christian, Junn, Alexandra, Kapur, Sahil, Kasrai, Leila, Kawamoto, Henry K., Jr, Khonsari, Roman, Kirschner, Richard E., Kondra, Katelyn, Levine, Jamie P., Li, Jingtao, Losee, Joseph E., Mann, Robert Joseph, Manson, Paul N., Massenburg, Benjamin B., Mathijssen, Irene, Menick, Frederick J., Mericli, Alexander F., Monson, Laura A., Morrison, Edwin, Mulliken, John B., Pannuto, Lucia, Paternoster, Giovanna, Persing, John A., Podolsky, Dale J., Pribaz, Julian J., Purnell, Chad A., Rastogi, Pratik, Rodriguez, Eduardo D., Schuster, Lindsay A., Shakir, Afaaf, Shakir, Sameer, Shetye, Pradip R., Simon, Daniel, Smetona, John T., Sommerlad, Brian, Stanton, Eloise, Susarla, Srinivas M., Taub, Peter J., Taylor, Jesse A., Torok, Kathryn S., Tse, Raymond W., Urata, Mark, Vargo, James D., Washington, George, Wolkswinkel, Erik, Yen, Stephen, Yu, Peirong, Zuker, Ronald M., Agarwal, Cori A., Altman, Andrew M., Atia, Andrew Nagy, Attinger, Christopher E., Atves, Jayson N., Brorson, Håkan, Cederna, Paul S., Chang, Brian L., Chang, David W., Chen, Hung-Chi, Chen, Wei F., Dekker, Paige K., Deldar, Romina, Dumanian, Gregory A., Evans, Karen K., Fahradyan, Vahe, Falola, Reuben A., Garza, Rebecca M., Germann, Günter K., Haffner, Zoe K., Andres Hernandez, J., Hollenbeck, Scott Thomas, Hontscharuk, Rayisa, Innocenti, Marco, Johns, Dana N., Khansa, Ibrahim, Kim, Kevin G., Kleiber, Grant M., Kovach, Stephen, Kumar, Nishant Ganesh, Kung, Theodore A., Lee, Raphael C., Scott Levin, L., Li, Alexander Y., Lin, Walter C., Lombana, Nicholas F., Louie, Otway, Lucattelli, Elena, Maldonado, Andrés A., Miller, John D., Mohos, Balazs, Muthukumar, Vamseedharan, Narasiman, Venkateshwaran, Orfahli, Lynn M., Parikh, Rajiv P., Puri, Vinita, Raja Sabapathy, S., Said, Hakim, Safa, Bauback, Saint-Cyr, Michel H., Sauerbier, Michael, Sayyed, Adaah A., Schechter, Loren, Scott, Kaylee B., Raja Shanmugakrishnan, R., Sharif-Askary, Banafsheh, Song, David H., Song, Ping, Steinberg, John S., Suh, Hyunsuk Peter, Tang, Yueh-Bih, Tzou, Chieh-Han John, Vrouwe, Sebastian Q., Gabriel, Allen, Allen Sr., Robert J., Angrigiani, Claudio, Auclair, Eric Michel, Azoury, Saïd C., Baker, Nusaiba F., Bengtson, Bradley P., Bistoni, Giovanni, Blasdel, Gaines, Blondeel, Phillip, Bluebond-Langner, Rachel, Bolletta, Elisa, Bradley Calobrace, M., Calva-Cerquiera, Daniel, Cargile, John C., Chevray, Pierre, Chi, David, Choi, Vincent J., Cissell, Matthew, Colakoglu, Salih, Colwell, Amy S., Cortes, Raul A., Clemens, Mark W., Crowley, Connor, Deva, Anand, de Vita, Roy, Egro, Francesco M., Eom, Jin Sup, Farhadi, Jian, Glicksman, Caroline A., Gould, Daniel J., Grufman, Vendela, Haddock, Nicholas T., Hall-Findlay, Elizabeth J., Hamdi, Moustapha, Hammond, Dennis C., Han, Hyunho, Hauch, Adam T., Hofer, Stefan O.P., Irigo, Marcelo, Kanchwala, Suhail K., Karp, Nolan S., Keane, Grace, Khavanin, Nima, Khouri, Roger Khalil, Kim, John Y.S., Koesters, Emma C., Laun, Jake C., Mc Guire, Patricia, Ledo, Gustavo Jiménez Muñoz, O'Neill, Anne C., Piatkowski, Andrzej, Lentz, Rachel, Lipa, Joan E., Losken, Albert, Mallucci, Patrick, Manahan, Michele Ann, Masià, Jaume, Mays, Chester J., Maxwell, Patrick, Mc Ardle, Adrian, Mc Carthy, Colleen M., Munhoz, Alexandre, Mesbahi, Alex, Movassaghi, Kiya, Myckatyn, Terence M., Nava, Maurizio, Nahabedian, Maurice Y., Opsomer, Dries, Parikh, Janak A., Patel, Ketan M., Patel, Nakul Gamanlal, Pazmiño, Pat, Perez, Justin L., Pomata, Cristhian D., Ramakrishnan, Venkat V., Rancati, Agustin, Rancati, Alberto, Randquist, Charles, Rosson, Gedge D., Peter Rubin, J., Sacks, Justin M., Sanz, Javier, Hilaire, Hugo St., Salibian, Ara A., Sbitany, Hani, Shauly, Orr, Spiegel, Aldona J., Spring, Michelle, Stevens, Grant, Stewart, Christopher N., Tanna, Neil, Tenenbaum, Marissa, Teotia, Sumeet S., Tesfaye, Eliora A., Thekkinkattil, Dinesh, Venturi, Mark L., Vidya, Raghavan, Vieira, Brittany L., Michelina, Veronica Vietti, Wu, Liza C., Yemc, Louisa, Ahn, Hee Chang, Baumgartner, Rita E., Berger, Aaron, Berridge, Anna, Bindra, Randy R., Bini, Nathalie, Brandacher, Gerald, Brown, Amanda, Brown, Hazel, Calabrese, Sara, Calfee, Ryan P., Carr, Logan W., Chan, James K-K., Chang, James, Chase, Robert A., Chen, Shanlin, Chim, Harvey, Chong, Alphonsus K.S., Chuang, David Chwei-Chin, Coert, J. Henk, Cox, Christopher, Curtin, Catherine, Dahlin, Lars B., De, Soumen Das, Davidge, Kristen M., Dell, Paul C., Dengler, Jana, Dumanian, Gregory Ara, Farnebo, Simon, Fok, Margaret, Fox, Ida K., Fox, Paige M., Friedrich, Jeffrey B., Garcia, Brittany N., A. Goldfarb, Charles, Staines, Kimberly Goldie, Haas-Lützenberger, Elisabeth, Haase, Steven C., Harhaus, Leila, Hagert, Elisabet, Hammert, Warren C., Hazell, Dennis, Henta, Vincent, Hill, Jonay, Hentz, Vincent R., Jaloux, Charlotte, Jones, Neil F., Jordan, Sumanas W., Kakinoki, Ryosuke, Kang, Jason R., Ko, Jason Hyunsuk, Kulber, David A., Kumar, Bhaskaranand, Lalonde, Donald, Lam, Wee Leon, Leclerq, Caroline, Lee, Dong Chul, Andrew Lee, W.P., Legrand, Anais, Liao, Janice, Lopez, Christopher D., Lopez, Joseph, Lu, Johnny Chuieng-Yi, Mailey, Brian A., Mau, Minnie, Mc Cabe, Steven J., Mc Cullough, Meghan C., Megerle, Kai, Moore, Amy M., Moore, Wendy, Moran, Steven L., Nanchahal, Jagdeep, T. Netscher, David, Neumeister, Michael W., van Nieuwenhoven, Christianne A., Oberg, Kerby C., O'Brien, Andrew, Park, Eugene, Pet, Mitchell A., Prommersberger, Karl-Josef, Quick, Tom J., Ramanuj, Parashar, Reinholdt, Carina, Sammer, Douglas M., Sandvall, Brinkley K., Satteson, Ellen, Sen, Subhro K., Sharma, Pundrique, Shen, Xiao Fang, Shores, Jamie T., Singh, Vanila M., Smith, Gillian D., Tadisina, Kashyap K., Taghinia, Amir H., Tan, David M.K., Tang, Jin Bo, Thorfinn, Johan, Tian, Xiaofei, Tonkin, Michael, Upton, Joseph, Valero-Cuevas, Francisco J., Venkatramani, Hari, Vedder, Nicolas B., Yeung, Celine, Werker, Paul M.N., Yao, Jeffrey, and Yoon, Jung Soo
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- 2024
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7. 14. CRISPR/Cas9 Edited Dendritic Cells Accelerate Wound Healing By Targeting Wound Fibroblasts Via Growth Factor Signaling.
- Author
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Henn, Dominic, Zhao, Dehua, Sivaraj, Dharshan, Trotsyuk, Artem, Fischer, Katharina, Fehlmann, Tobias, Levi, Benjamin, Keller, Andreas, Chen, Kellen, Qi, Lei S., and Gurtner, Geoffrey C.
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- 2024
- Full Text
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8. Elevated Shear Stress Modulates Heterogenous Cellular Subpopulations to Induce Vascular Remodeling.
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Fischer KS, Henn D, Zhao ET, Sivaraj D, Litmanovich B, Hahn WW, Hostler AC, Mojadidi SM, Gonzalez J, Knochel AB, Mora Pinos MG, Holley J, Kussie H, Granoski M, Yasmeh JP, Kneser U, Chen K, and Gurtner GC
- Abstract
Rationale: Elevated shear stress (ESS) induces vascular remodeling in veins exposed to arterial blood flow, which can lead to arteriovenous (AV) fistula failure. The molecular mechanisms driving remodeling have not been comprehensively examined with a single-cell resolution before. Objective: Using an in vivo animal mode, single-cell RNA sequencing, and histopathology, we precisely manipulate blood flow to comprehensively characterize all cell subpopulations important during vascular remodeling. Methods: AV loops were created in saphenous vessels of rats using a contralateral saphenous vein interposition graft to promote ESS. Saphenous veins with no elevated shear stress (NSS) were anastomosed as controls. Findings: ESS promoted transcriptional homogeneity, and NSS promoted considerable heterogeneity. Specifically, ESS endothelial cells (ECs) showed a more homogeneous transcriptional response promoting angiogenesis and upregulating endothelial-to-mesenchymal transition inhibiting genes ( Klf2 ). NSS ECs upregulated antiproliferation genes such as Cav1 , Cst3 , and Btg1 . In macrophages, ESS promoted a large homogeneous subpopulation, creating a mechanically activated, proinflammatory and thus proangiogenic myeloid phenotype, whereas NSS myeloid cells expressed the anti-inflammatory and antiangiogenetic marker Mrc1 . Conclusion: ESS activates unified gene expression profiles to induce adaption of the vessel wall to hemodynamic alterations. Targeted depletion of the identified cellular subpopulations may lead to novel therapies to prevent excessive venous remodeling, intimal hyperplasia, and AV fistula failure.
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- 2024
- Full Text
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9. Skin treatment with non-thermal plasma modulates the immune system through miR-223-3p and its target genes.
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Engel A, Ludwig N, Grandke F, Wagner V, Kern F, Fehlmann T, Schmartz GP, Aparicio-Puerta E, Henn D, Walch-Rückheim B, Hannig M, Rupf S, Meese E, Laschke MW, and Keller A
- Subjects
- Animals, Mice, Gene Expression Profiling, Wound Healing drug effects, Signal Transduction, Immune System metabolism, MicroRNAs genetics, Skin metabolism, Plasma Gases pharmacology, Gene Expression Regulation drug effects
- Abstract
Non-thermal plasma, a partially ionized gas, holds significant potential for clinical applications, including wound-healing support, oral therapies, and anti-tumour treatments. While its applications showed promising outcomes, the underlying molecular mechanisms remain incompletely understood. We thus apply non-thermal plasma to mouse auricular skin and conducted non-coding RNA sequencing, as well as single-cell blood sequencing. In a time-series analysis (five timepoints spanning 2 hours), we compare the expression of microRNAs in the plasma-treated left ears to the unexposed right ears of the same mice as well as to the ears of unexposed control mice. Our findings indicate specific effects in the treated ears for a set of five miRNAs: mmu-miR-144-5p, mmu-miR-144-3p, mmu-miR-142a-5p, mmu-miR-223-3p, and mmu-miR-451a. Interestingly, mmu-miR-223-3p also exhibits an increase over time in the right non-treated ear of the exposed mice, suggesting systemic effects. Notably, this miRNA, along with mmu-miR-142a-5p and mmu-miR-144-3p, regulates genes and pathways associated with wound healing and tissue regeneration (namely ErbB, FoxO, Hippo, and PI3K-Akt signalling). This co-regulation is particularly remarkable considering the significant seed dissimilarities among the miRNAs. Finally, single-cell sequencing of PBMCs reveals the downregulation of 12 from 15 target genes in B-cells, Cd4+ and Cd8+ T-cells. Collectively, our data provide evidence for a systemic effect of non-thermal plasma.
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- 2024
- Full Text
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