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ABSTRACT Klebsiella pneumoniae is a prominent Gram-negative and encapsulated opportunistic pathogen that causes a multitude of infections such as severe respiratory and healthcare-associated infections. Despite the widespread anti-microbial resistance and the high mortality rate, currently, no clinically vaccine is approved for battling K. pneumoniae. To date, messenger RNA (mRNA) vaccine is one of the most advancing technologies and are extensively investigated for viral infection, while infrequently applied for prevention of bacterial infections. In the present study, we aim to construct a new mRNA vaccine encoding YidR or combining with a tissue plasminogen activator signal sequence for preventing K. pneumoniae infection. Adaptive immunity was determined in mRNA vaccines-immunized mice and the protective effects of mRNA vaccines were evaluated in K. pneumoniae infected models. The results showed that lipid nanoparticle (LNP)-YidR-mRNA vaccine was produced with good morphology, high the encapsulation efficiency, and the specific antigen was highly expressed in cells in vitro. In addition, immunization with either LNP-YidR or LNP-YidR-SP elicited a Th1-biased immune response, reduced bacterial load, and provided broad protection in the lung infection models. Importantly, the LNP-YidR-SP mRNA vaccine induced strong adaptive humoral and cellular immunity and increased the survivability of mice compared to the other groups. Our findings serve as a focal point for developing a potential mRNA vaccine against K. pneumoniae, indicating the potential of mRNA vaccines for improving next-generation bacterial vaccine.IMPORTANCEK. pneumoniae is a notorious and clinical bacterium that is evolving in community-acquired and nosocomial settings. This opportunistic pathogen causes severe infectious diseases, including urinary tract infection and pneumonia, and causes a concerning global public burden. Despite efforts having been created to develop different types of K. pneumoniae vaccines, there is no licensed vaccine for preventing K. pneumoniae infection. Therefore, to develop an effective tactic is essential to combat K. pneumoniae-caused diseases. This study provides a novel vaccine strategy against K. pneumoniae and a potent platform to elicit high levels of humoral and cell-meditated immunity.

Close-distance coal seams are common in underground mining, and their spacing is short, which produces strong mining disturbance. In instances where the upper seam has been mined and a goaf has formed, a notable issue arises during the lower seam’s mining, characterized by substantial deformation of the roadway along the goaf. Field exploration and three-dimensional geological modeling have revealed that the fourth and sixth working faces and pillar of seam No. 5 are all under seam No. 2’s goaf, with an average distance of 16.70 m. Simultaneously, the double compression effect of the pillar, induced by the linkage rotation of key blocks of the lower and upper seams, is analyzed. The induction mechanism and path of the large deformation are expounded. It is thus proposed that the pillar’s width should be determined by gob-side entry, driving beneath the goaf, with the roof near the pillar being cut off in advance to realize the path of cutting off the compressed pillar. Through the simulation comparison of five kinds of pillar width combined with engineering practice, it has been determined that the best width is 8 m, and the abutment pressure is distributed in a double-peak saddle shape, with the result that the load-bearing ability is notably significant. Through the comparative simulation of roof-cutting, it was found that roof-cutting helps the roof to collapse near the pillar-side and decreases the vertical stress peak to 16.46 MPa, the shear stress peak to 5.93 MPa, and the J2 peak to 7.23 × 1013 Pa, which further alleviates the pressure on the pillar. In the field, the haulage roadway’s roof was cut by two-way shaped-charge blasting, and the sandy mudstone (5.90 m) was successfully cut off. Concurrently, anchor cable reinforcement was implemented on the roof and two ribs of the ventilation roadway in proximity to the pillar, thereby ensuring stabilization and mitigating the mining effect. The engineering research provides a case and scheme reference for the operation of gob-side entry driving beneath close-distance goafs worldwide.

ObjectivesThis study aimed to assess any possible links between visceral fat accumulation and an elevated prevalence of osteoarthritis (OA) in the elderly.Methods3,779 subjects aged 65 years or older from the National Health and Nutrition Examination Survey (NHANES) 1999–2018 were finally included, of whom 516 had OA. The identification of patients diagnosed with OA was conducted using questionnaire data. The visceral adiposity index (VAI) was computed utilizing body mass index (BMI), waist circumference (WC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C). To investigate the relationship between VAI and OA, weighted multivariable logistic regression analysis, restricted cubic spline (RCS), subgroup analyses, and interaction tests were carried out.ResultsMultivariate logistic regression analysis showed that the increase in VAI is accompanied by an increased prevalence of OA after fully correcting for variables. The top quartile of VAI had a prevalence that was 110% higher than the lowest quartile. A non-linear positive correlation between VAI and OA was found in the RCS.ConclusionThis study suggests a potential correlation between elevated VAI and increased prevalence of OA in older adults, and that lowering VAI may have an impact on the prevalence of OA.