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Background: Ensuring consistent adherence to antiretroviral therapy (ART) is crucial for effective HIV treatment and achieving viral suppression. Within prisons, the prevalence of HIV is notably high, and incarcerated individuals face an increased risk of transmitting the virus both during and after incarceration. However, facilitators and barriers to ART adherence among these individuals in low- and middle-income countries remain inadequately explored. This study applied the Social Ecological Model (SEM) to investigate how various individual, interpersonal, organizational, community and policy-level factors impact ART adherence among incarcerated populations in Iran during and post-incarceration., Methods: This study employed a phenomenological qualitative approach using semi-structured interviews to gather insights. The research population consisted of people living with HIV (PLHIV) who had experienced incarceration and had been prescribed ART during their latest incarceration. Eleven PLHIV from two prisons located in Kerman and Tehran, Iran, formed the study group. Qualitative findings from the interviews were analyzed using a thematic approach. The findings were organized within the SEM framework to highlight key themes influencing ART adherence during and after incarceration., Results: Participants had an average age of 45.1 years (± 5.6). Various factors influence ART adherence during and post-incarceration. Participants highlighted the individual (e.g., HIV knowledge, previous treatment history, mental and physical health), interpersonal (e.g., family, friends, other incarcerated people, and prison health staff), organizational (e.g., ART treatment interventions, methadone maintenance therapy, and other health protocols), community (e.g., stigma, social isolation, discrimination and lack of access to community health services), and policy (e.g., financial interventions, and providing shelters) level factors influence ART adherence during and post-incarceration., Conclusions: This study provides insights into the multi-level approach to ART adherence among PLHIV during and post-incarceration. It recommends implementing public health activities at the proposed multi-levels to maximize the synergies of intervention for the greatest impact., Competing Interests: Declarations. Ethics approval and consent to participate: Participation in this study was entirely voluntary and oral consent was acquired at the beginning of qualitative interviews. The Research Ethics Committee of Kerman University of Medical Sciences has approved the project following the tenets of the Helsinki Declaration and the national ethical guideline for medical research (IR.KMU.REC.1400.674). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Purpose: Metabolic syndrome (MetS) is a condition of high prevalence worldwide associated with increased risk of cardiovascular disease. The predictive value of novel indices of combined anthropometric and serum lipid parameters as predictors of MetS is, to our knowledge, unexplored. We aimed to assess the 10-year predictive value of three indices of triglyceride-glucose index (TyG), visceral adiposity index (VAI), and lipid accumulation product (LAP) for incidence of MetS in Southeastern Iran., Methods: A total of 1084 healthy adults aged 15 to 75 years (among 5895 participants in the KERCADR study) were followed for 10 years. Baseline calculations were performed for LAP, TyG, and VAI. Logistic regression models were employed to assess the association of these indices with the incidence of MetS during the follow-up period. We also evaluated the cut-off value and predictive performance of these indices for predicting MetS using the area under their ROC curves (AUCs)., Results: During 10-year follow-up, 506 MetS cases were documented. The adjusted odds ratio (AOR) with 95% confidence intervals (CI) of the 10-year MetS risk for LAP, VAI, and TyG was as follows: 3.13 (1.79, 5.48), 2.57 (1.60, 4.12), and 2.20 (1.45, 3.35), respectively. In both sexes, LAP exhibited the highest performance (AUC values) compared to the other two composite indices and to the traditional single indices, such as BMI and WC., Conclusion: Overall, this study revealed significant elevations of LAP, TyG, and VAI in individuals with MetS. LAP demonstrated the highest AUC, over the new and traditional single risk indices of MetS. These findings underscore the potential predictive value of LAP in assessing the 10-year incidence of MetS., Competing Interests: Declarations. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests. Research involving human participants and/or animals: Approval for the study protocol was provided by the Ethics Committee of Kerman University of Medical Sciences (ethics code: IR.KMU.REC.401.286) in accordance with the Declaration of Helsinki. Informed consent: Written informed consent was obtained from all participants in the KERCADR study., (© 2024. The Author(s), under exclusive licence to Hellenic Endocrine Society.)

This review delves into the potential of antimicrobial peptides (AMPs) as promising candidates for combating arboviruses, focusing on their mechanisms of antiviral activity, challenges, and future directions. AMPs have shown promise in preventing arbovirus attachment to host cells, inducing interferon production, and targeting multiple viral stages, illustrating their multifaceted impact on arbovirus infections. Structural elucidation of AMP-viral complexes is explored to deepen the understanding of molecular determinants governing viral neutralization, paving the way for structure-guided design. Furthermore, this review highlights the potential of AMP-based combination therapies to create synergistic effects that enhance overall treatment outcomes while minimizing the likelihood of resistance development. Challenges such as susceptibility to proteases, toxicity, and scalable production are discussed alongside strategies to address these limitations. Additionally, the expanding applications of AMPs as vaccine adjuvants and antiviral delivery systems are emphasized, underscoring their versatility beyond direct antiviral functions., Competing Interests: Declarations. Conflict of Interest: The authors declare no competing interests. Institutional Review Board Statement: This research involved no human participants or animals., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

High quality baseflow data is important for advancing water resources modeling and management, as it captures the critical role of groundwater and delayed sources in contributing to streamflow. Baseflow is the main recharge source of runoff during the dry period, particularly in understanding the interaction between surface water and groundwater systems. This study focuses on estimating baseflow using deep learning algorithms that enhance the estimation capabilities in both gauged and ungauged basins. Recognizing the shortage in accessible high quality daily baseflow data, our objective is to generate a daily baseflow dataset across the contiguous United States (CONUS) for 1661 basins from 1981 to 2022. This dataset provides valuable information for earth and environmental scientists, and water resource managers, enhancing our understanding of the water cycle. It also provides an important foundation for enhancing the study of baseflow contributions to extreme events such as droughts and floods. The dataset can be used as a new benchmark for future studies aimed at improving hydrological predictions and managing water resources more effectively., Competing Interests: Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)

Cryptosporidium spp. is an opportunistic protozoan parasite that primarily affects immunocompromised individuals, including those with colorectal cancer (CRC). This study investigates the prevalence and clinical significance of Cryptosporidium spp. in CRC patients, including both the gastrointestinal and respiratory systems. A total of 108 CRC patients and 102 healthy controls were enrolled, and stool, sputum, and saliva samples were analyzed using both microscopy and qPCR methods. Cryptosporidium spp. were detected in 13% of CRC patients' stool samples, compared to 2% in the control group, demonstrating a statistically significant difference (P = 0.0013). In contrast, there was no significant difference in Cryptosporidium spp. positivity between CRC patients and controls in sputum or saliva samples. Strikingly, one CRC patient had Cryptosporidium spp. in both sputum and saliva samples in addition to stool, suggesting potential secondary pulmonary involvement. The findings highlight the importance of screening for Cryptosporidium spp. in CRC patients, particularly those with respiratory symptoms, advanced-stage disease, or left-sided tumors, to ensure timely diagnosis and management of this opportunistic infection. Further research is needed to clarify the pathogen's role in colorectal carcinogenesis and the implications for patient outcomes., (Copyright © 2025 Wolters Kluwer Health, Inc. All rights reserved.)

Background: The role of direct oral anticoagulants (DOACs) in the treatment of left ventricular thrombus (LVT) after ST-elevation myocardial infarction (STEMI) remains uncertain., Aims: We aimed to compare the effect of rivaroxaban versus warfarin in patients with STEMI complicated by LVT., Methods: Adult patients with STEMI and two-dimensional transthoracic echocardiography showing LVT were assigned to rivaroxaban (15 mg once daily) or warfarin (international normalised ratio goal of 2.0-2.5) in an open-label, randomised clinical trial (RCT). A prospective pooled analysis was planned comparing DOAC- versus warfarin-based anticoagulation for the same indication. The main outcome of the RCT was complete LVT resolution at 3 months, determined by a blinded imaging core laboratory. Complete LVT resolution and bleeding were investigated in the pooled analysis., Results: A total of 50 patients (median age: 55 years, 18% females) were enrolled from June 2020 to November 2022. Three-month complete LVT resolution occurred in 19/25 (76.0%) patients assigned to rivaroxaban and 13/24 (54.2%) assigned to warfarin (relative risk [RR] 1.40, 95% confidence interval [CI]: 0.91-2.15; p=0.12) with no thrombotic or major bleeding events. Pooled analysis showed numerically better complete LVT resolution with DOACs (rivaroxaban and apixaban; 93/115 [80.8%] vs 79/112 [70.5%], RR 1.14, 95% CI: 0.98-1.32; p=0.08) and less major bleeding (2/116 [1.7%] and 9/112 [8.0%], risk difference -0.06, 95% CI: -0.12 to 0.00; p=0.05) than with warfarin., Conclusions: Although the findings are limited by a small sample size, the results suggest that DOACs are safe with at least similar outcomes concerning LVT resolution and major bleeding compared with warfarin. (ClinicalTrials.gov: NCT05705089).

Extravasation injuries are prevalent in pediatric hospitals and often result in tissue damage and extended hospital stays. However, limited data exist regarding risk factors for extravasation in children. This study aimed to identify these risk factors in pediatric patients receiving intravenous (IV) therapy. This prospective cohort study included 500 children aged 1 month to 18 years who had undergone either central or peripheral catheterization and were admitted to the pediatric intensive care unit (PICU) at Imam Hossein Children's Hospital in Isfahan, Iran, between July 2020 and July 2021. Data were collected on age, gender, length of hospitalization, catheter type, instances of extravasation, drugs administered, and treatment protocols. The median age was 36 months, and approximately 56% of participants were male. Among the cohort, the prevalence of extravasation was 9.8% (49 patients). Female gender ( P = .002), central venous catheterization ( P = .002), prolonged hospitalization (P < .001), reduced consciousness ( P = .012), and the use of taping for vein fixation ( P = .004) were all significantly associated with a higher risk of extravasation. This study underscores the need for health care providers to carefully assess risk factors for extravasation to mitigate injury risk, particularly in vulnerable pediatric populations., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Aims: This study aimed to improve rivaroxaban delivery by optimising solid lipid nanoparticles (SLN) for minimal mean diameter and maximal entrapment efficiency (EE), enhancing solubility, bioavailability, and the ability to cross the blood-brain barrier., Methods: A central composite design was employed to synthesise 32 SLN formulations. Response surface methodology (RSM) and artificial neural networks (ANN) models predicted mean diameter and EE based on five independent variables., Results: The optimised SLN formulation achieved a mean particle diameter of 159.8 ± 15.2 nm, with a Polydispersity index of 0.46, a zeta potential of -28.8 mV, and an EE of 74.3% ± 5.6%. The ANN model showed superior accuracy for both mean diameter and EE, outperforming the RSM model. Structural integrity and stability were confirmed by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and Fourier-transform infrared spectroscopy (FTIR)., Conclusion: The high accuracy of the ANN model highlights its potential in optimising pharmaceutical formulations and improving SLN-based drug delivery systems.

This review explores the establishment of diagnostic reference levels (DRLs) for pediatric brain computed tomography (CT) examinations in Saudi Arabia and compares them with nine other countries. An extensive search strategy was employed across various databases, resulting in the inclusion of 9 studies. The studies included patient-based and phantom-based investigations into DRLs, highlighting variations across age groups and countries. Findings suggest notable differences in CT dose index (CTDI mGy) and dose length product (DLP mGy.cm) values. There was a difference in the classification of age group between Saudi food and drug administration (SFDA) and literature. For the age groups 0-5 years and 6-15 years, the DRLs for the SFDA were as follows: CTDI (28 and 42 mGy) and DLP (482 and 697 mGy cm). The discussion emphasizes the importance of age-specific DRLs to optimize radiation doses while ensuring patient safety and diagnostic efficacy. Recommendations include adopting globally accepted standards for dose optimization and continued research into factors influencing DRL variations. Limitations include varying age groupings among studies and limited access to some relevant literature. Overall, this study underscores the importance of standardizing DRLs for pediatric CT to improve patient care and safety., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025 Elsevier Ltd. All rights reserved.)

Background and Purpose: Despite the increasing integration of information technologies in healthcare settings, limited attention has been given to understanding technostress among health practitioners in hospitals. This study aims to assess the prevalence of technostress creators among health practitioners and explore potential factors contributing to its occurrence, with the ultimate goal of informing strategies to mitigate its impact., Method: Data were collected through a validated questionnaire administered to health practitioners at Tehran Apadana Hospital in Iran. The questionnaire encompassed demographic information and technostress assessment items. Statistical analysis was conducted using SPSS software to examine the relationship between technostress levels and demographic characteristics., Findings: The analysis revealed that approximately 41% of health practitioners experienced medium levels of technostress, with 36% reporting high levels and 23% reporting low levels (χ2F = 55.4; p < 0.001). Notably, technology uncertainty emerged as the primary driver of technostress, followed by techno-overload, techno-complexity, techno-insecurity, and techno-invasion. Surprisingly, no significant relationship was found between technostress levels and demographic characteristics., Conclusion: The study underscores the pressing need to address the prevalent medium to high levels of technostress among health practitioners in hospital environments. By shedding light on the key stressors and their distribution, these findings can inform human resources management strategies within healthcare systems to effectively support practitioners in navigating and managing technostress challenges., Competing Interests: Declarations. Ethics approval and consent to participate: In the research study conducted, informed consent was obtained from the provider participants who completed the questionnaires. Participants were assured that their information would be kept confidential throughout all phases of the study, and their privacy was respected. The Tarbiat Modares University Ethics Committee/IRB has waived the requirement for ethical approval for this study, as it did not involve patients. All methods employed in the study were conducted in accordance with relevant guidelines and regulations, including adherence to the Declaration of Helsinki. Confidentiality of participant information was maintained, and all procedures were carried out with the utmost respect for ethical considerations and participant rights. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)

Burn wounds are challenging to treat due to considerable tissue damage and fluid loss. Creating wound dressings from natural and biological materials makes it possible to treat wounds and promote rapid epithelialization to speed healing and restore skin function. As a result, the ability of a collagen scaffold (Col) made from rainbow trout (Oncorhynchus mykiss) and putative bioactive phytochemical components from a Sargassum glaucescens (S. glaucescens) extract (SGE) to promote burn wound healing was assessed in this work. Synthesized collagen (40 mg/ml)/SGE (1-3 mg/ml) samples were then characterized physiochemically and physiologically. The physicochemical examination validated the bioactive component of SGE, the type of collagen (type I, α1, and α2), the successful incorporation of SGE into collagen scaffolds (Col/SGE), the thermal stability, and excellent hydrophilicity and water absorption capacity of produced scaffolds. Moreover, biological experiments approved the excellent antioxidant and antibacterial activity of SGE, structural stability improvement against degradation, and cell proliferation enhancement without cell toxicity. The results showed the Col/SGE 3 mg/ml sample also had the highest level of cell activity, according to the antibacterial and cell viability assays. Additionally, using Col/SGE in vivo on burn wounds in rat models demonstrated a quicker rate of wound healing with stronger re-epithelialization and dermal remodeling, fewer inflammatory cells, more fibroblast cells, and great collagen buildup. Therefore, since the collagen/SGE scaffold is structurally stable and can potentially promote cell proliferation without causing cell toxicity, the acquired results suggested that it may significantly impact wound healing., Competing Interests: Declarations. Ethics Approval: All animal experiments were carried out under permission of the ethical committee of Iran University of Medical Sciences (IR.IUMS.REC.1400.525). Conflict of Interest: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Purpose: Preimplantation aneuploidy in humans is one of the primary causes of implantation failure and embryo miscarriage. This study was conducted to gain insight into gene expression changes that may result from aneuploidy in blastocysts through RNA-Seq analysis., Methods: The surplus embryos of preimplantation genetic testing for aneuploidy (PGT-A) candidate couples with normal karyotype and maternal age < 38 were collected following identical ovarian stimulation protocol. The embryos were selected based on trophectoderm biopsy and array comparative genomic hybridization in three groups: normal group, small chromosomes aneuploidy group (SCA), including single aneuploidy for small chromosomes 16, 20, 21, 22, and other chromosomes aneuploidy group (OCA), including single aneuploidy for other chromosomes., Results: Principal component analysis revealed overall differentiation of transcriptome of the groups, confirming embryo classification. The Gene Ontology indicated that transcription, ubiquitination, autophagy, and DNA repair pathways were upregulated in aneuploid embryos. The overexpression of five genes, UBE2E2 and VPS4A, BUB1B, CDCA8, and COX14 was confirmed by quantitative real-time PCR. Additionally, overexpression was observed in translation and protein synthesis pathways in aneuploid embryos. Mitochondrial pathway upregulation was notable in both SCA and OCA groups, while the apoptosis pathway was overexpressed only in the OCA group. Only cellular lipid synthesis pathway differed between SCA and OCA, the two aneuploid groups., Conclusions: This study highlights the impact of aneuploidy on the gene expression in blastocysts independent of aneuploidy type and paves the way for understanding the molecular mechanisms underlying the generation of aneuploidy., Competing Interests: Declarations. Ethics approval: This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Institutional Research Ethics Committee (approval code: IR.ACECR.ROYAN.REC.1397.210). Conflict of interest: The authors declare no conflict of interest., (© 2025. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

In this paper, we propose and theoretically investigate a novel multimode refractive index (MMRI) plasmonic optical sensor for detecting various brain cancer cells, leveraging the unique capabilities of split ring resonators (SRRs). The sensor, simulated using the finite-difference time-domain (FDTD) method, exhibits dual resonance modes in its reflection spectrum within the 1500 nm to 3500 nm wavelength range, marking a significant advancement in multimode plasmonic biosensing. Through detailed parametric analysis, we optimize critical dimensional parameters to achieve superior performance. The novelty of this work lies in the dual-mode sensing mechanism, which enables robust detection by exploiting the resonance characteristics of gold, silver, and aluminum. These materials provide tunable and highly sensitive interactions with light, enhancing the sensor's adaptability for a wide range of applications. The results reveal exceptional sensitivity values of 1778.3 nm/RIU, a limit of detection (LOD) of 0.016 RIU, and a high figure of merit (FOM) of 7 RIU -1 , along with a quality factor (QF) of 11.7 in the first resonance mode. The findings show that the designed optical biosensor exhibits high sensitivity, a good LOD, and an acceptable FOM in both resonance modes. So, this work paves the way for future research and development of susceptible, multimode optical sensors for medical diagnostics. The results indicate that the proposed sensor operates effectively across a range of temperatures and angles of radiant light, demonstrating its independence from these variables. This reliability in performance underscores its potential for use in diverse environments, making it a dependable tool for detecting biological samples, including brain cancer cells, irrespective of external conditions., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Background: Hand-arm Vibration Syndrome (HAVS) is a disorder caused by prolonged exposure to hand-held vibrating instruments, commonly observed in industrial contexts such as mining, construction, and manufacturing. It involves symptoms affecting the musculoskeletal, neurological, and vascular systems of the arm and hand., Purpose: The main aim of this study is to determine the prevalence of HAVS among laborers working in the Khewra salt mines., Study Design: This study utilized a cross-sectional observational design., Methods: A total of 140 drill machine operators from the Khewra salts mines were selected through purposive sampling. Handgrip strength was assessed using the Camry-EH101 hand dynamometer and the data was collected through structured questionnaire focusing on neurological, vascular and musculoskeletal symptoms. The prevalence of HAVS was determined from the collected data and the severity of symptoms was as analyzed across different age groups and working conditions., Results: Out of the participants, n = 121 (86.4%) were found to be right-hand dominant, with average grip strength of 40.9 ± 3.1 kg, while n = 19 (13.6%) were left hand dominant, with average grip strength of 45.9 ± 2.6 kg. Compared to normative data, right-hand dominant workers showed a 15% reduction in grip strength, while left-hand dominant workers showed a 5% decreased. The most common reported neurological symptoms included prolonged hand vibration (52.9%), tingling sensation (60%) and numbness (46.4%). Vascular symptoms included discoloration of fingers (50%), felling cold (48.6%) and needle like sensations (45.7%). Musculoskeletal symptoms included reduced grip strength (51.4%), hand discomfort (46.4%) and hand cramping, tiredness or itching (46.4%)., Conclusion: The high prevalence of HAVS among miners emphasizes the importance of targeted clinical interventions and workplace policies modifications to reduce vibration exposure and improve occupational health., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interests., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Lung Ischemia-reperfusion injury (LIRI) is a risk during lung transplantation that can cause acute lung injury and organ failure. In LIRI, the NF-E2-related factor 2(Nrf2)/ Kelch-like ECH-associated protein 1 (Keap1) signaling pathway and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway are two major pathways involved in regulating oxidative stress and inflammation, respectively. Myrtenol, a natural compound with anti-inflammatory and antioxidant properties, has potential protective effects against IRI. This study aimed to explore the impact of myrtenol encapsulated within niosomes on the prevention of LIRI and examine the role of the two pathways mentioned in this process. Wistar rats were segregated into four groups. Animals received the myrtenol (MN) (32 mg/kg) or vehicle through daily inhalation for a week before LIRI. Expression of IκB, p-IκB, Nrf2, Keap1, Heme Oxygenase-1(HO-1), NF-κB signaling proteins, reactive oxygen species (ROS) level, caspase-3 expression, arterial blood gases, lung edema, and histopathological indices were assessed. Niosomal myrtenol significantly reduced lung edema, ROS, Keap1, p-IκB, NF-kB, Caspase-3, PaCO2 (the carbon dioxide pressure in arterial blood), and histopathological indices. Additionally, the expression of IκB, Nrf2, HO-1, and PaO2 (the oxygen pressure in arterial blood) increased significantly in the pretreated group compared to the IR group. Overall, inhalation of the niosomal myrtenol protects against lung ischemia-reperfusion injury, presumably through the balance between Nrf2/Keap1 and NF-κB pathways. The findings suggest that the niosomal form of myrtenol may be a potential candidate for developing new drugs to prevent and treat LIR damage., Competing Interests: Declarations. Ethical approval: The experiments described in this work were conducted in accordance with the guidelines on ethical standards for investigation of animals. The protocol was reviewed and approved by the Ethic Committee of Kerman University of Medical Sciences, with the approval number: IR.KMU.REC.1400.056. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Herein, Polydopamine-modified microgels and microgels incorporated with superficial epoxy groups were synthesized and applied as precursors for the fabrication of four granular hydrogels. To enhance the tissue adhesiveness, a ternary deep eutectic solvent was synthesized to activate the muscle amine functional groups facilitating the formation of robust NC bonds at ambient conditions. At a certain shear rate of 10 s -1 , hydrogel DMG displayed a viscosity of 9×10 3  Pa/s, representing the highest complex viscosity among the tested hydrogels primarily driven by quinone groups in PDA which enhanced reversible interactions, thereby increasing particle cohesion. Moreover, the intersection point escalating from about 4×10 3 to approximately 9×10 4 as the concentration of DMG increased from 0 % (for MG) to 70% (7D3MG) by weight. There was a decrease in adhesion strength from 0.45 ± 0.08 N in MG to 0.39 ± 0.16 N, 0.35± 0.18 N, and 0.33 ± 0.15 N for 3D7MG, 7D3MG, and DMG respectively, suggesting that MG was capable of forming numerous covalent bonds, thereby enhancing its adhesion to the substrate. The type of eutectic mixture affected the electrical conductivity and a very important point was the changes in resistance value with time. For MG catalyzed by [DES] AZG , the resistance increased only by 1.3 % (from 3.37 to 3.81 kΩ) at day 3 and 37.09 % (from 3.37 to 4.62 kΩ) at day 5. The 3D7MG hydrogel exhibited superior therapeutic efficacy toward diabetic wound regeneration. The proliferation index value for 3D7MG-[DES] AZG and 3D7MG-[DES] AG were calculated 42.3 % and 58.6 %, respectively, while the control group exhibited a lower value of 37.8 %., Competing Interests: Declaration of competing interest The authors declare they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background: Age, race, ethnicity, and sex are important determinants of coronavirus disease of 2019 (COVID-19) outcomes. Older adults (65 years and older) are at the highest risk of COVID-19 morbidity and mortality. Analyzing their vaccine uptake by subclassifying demographics is rare and can assist vaccination policies. This study investigates COVID-19 dose 1 and 2 vaccine uptakes among them by race, ethnicity, and sex., Methods: Immunization registry data were used to calculate temporal changes in older adults' COVID-19 vaccine uptake by race, ethnicity, race-sex, and ethnicity-sex in Kentucky's most populous county, Jefferson County, during the first 6 quarters of the COVID-19 vaccination program., Results: By May 2022, the county's Asian residents had the highest dose 1 and 2 vaccination rates (97.0% and 80.4%), then White residents (90.0% and 80.2%). Black residents had one of the lowest COVID-19 vaccination rates (87.3% and 77.3%). The rate among Hispanic residents (82.0% and 66.4%) was considerably lower than non-Hispanic residents (90.2% and 80.1%). The rates were consistently lower in males., Conclusions: Racial, ethnic, and sex-based COVID-19 vaccine inequalities were largely maintained during the study period. Vaccine rollout practices and promotional programs should aim to boost the uptake of the COVID-19 vaccination among racial minority and male older adults., (Copyright © 2024 Association for Professionals in Infection Control and Epidemiology, Inc. All rights reserved.)

Breast cancer continues to be a significant contributor to global cancer deaths, particularly among women. This highlights the critical role of early detection and treatment in boosting survival rates. While conventional diagnostic methods like mammograms, biopsies, ultrasounds, and MRIs are valuable tools, limitations exist in terms of cost, invasiveness, and the requirement for specialized equipment and trained personnel. Recent shifts towards biosensor technologies offer a promising alternative for monitoring biological processes and providing accurate health diagnostics in a cost-effective, non-invasive manner. These biosensors are particularly advantageous for early detection of primary tumors, metastases, and recurrent diseases, contributing to more effective breast cancer management. The integration of biosensor technology into medical devices has led to the development of low-cost, adaptable, and efficient diagnostic tools. In this framework, electrochemical screening platforms have garnered significant attention due to their selectivity, affordability, and ease of result interpretation. The current review discusses various breast cancer biomarkers and the potential of electrochemical biosensors to revolutionize early cancer detection, making provision for new diagnostic platforms and personalized healthcare solutions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

In recent years, the widespread emergence of drug resistance in yeasts and filamentous fungi to existing antifungal armamentariums has become a severe threat to global health. There is also concern regarding increased rates of azole resistance in Aspergillus fumigatus and Terbinafine resistance in Trichophyton species. To overcome this concern of resistance to regular therapies, new antifungal drugs with novel and effective mechanisms are crucially needed. Herbal remedies may be promising strategies for the treatment of resistant infections. We aimed to investigate the in vitro and silico activity of piperlongumine against clinical azole susceptible/resistant A. fumigatus and terbinafine-susceptible/resistant Trichophyton species. In the current study, piperlongumine demonstrated potent antifungal activity, with minimum inhibitory concentrations (MICs) ranging from 0.016-4 μg/mL against Trichophyton isolates and 0.25-2 μg/mL for A. fumigatus isolates. Additionally, molecular docking studies indicated that piperlongumine has a strong binding affinity to the active sites of squalene epoxidase and sterol 14-alpha demethylase. However, further studies are warranted to correlate these findings with clinical outcomes and provide the basis for further investigations to pave the way for developing novel antifungal agents., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr. Iman Haghani reports financial support was provided by Mazandaran University of Medical Sciences. This research was financially supported by grant number 17588 from Mazandaran University of Medical Sciences. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Background: Chronic subdural hematoma (cSDH) is one of the most common neurosurgical conditions. Although surgical evacuation is still the gold standard for treatment, recent advances have led to the development of other management strategies, such as medical therapies and endovascular middle meningeal artery (MMA) embolization. Through this international survey, we investigated the global trends in cSDH management, focusing on medical and endovascular treatments., Design and Participants: A 14-question, web-based, anonymous survey was distributed to neurosurgeons worldwide., Results: Most responders do not perform MMA embolization (69.5%) unless for specific indications (29.6%). These indications include residual cSDH after surgical evacuation (58.9%) or cSDH in patients on antiplatelet medications to avoid surgical evacuation (44.8%). Survey participants from teaching versus non-teaching hospitals ( p  = 0.002), public versus private hospitals ( p  = 0.022), and Europe versus other continents ( p  < 0.001) are the most users of MMA embolization. A large number of participants (51%) declare they use a conservative/medical approach, mainly to avoid surgery in patients with small cSDH (74.8%)., Conclusions: This survey highlights the current trends of cSDH management, focusing on conservative and MMA embolization treatment strategies. Most responders prefer a conservative approach for patients with small cSDHs not requiring surgical evacuation. However, in higher-risk scenarios such as residual hematomas after surgery or patients on antiplatelet medications, MMA embolization is regarded as a reasonable option by participants. Future studies should clarify the indications of MMA embolization, including appropriate patient selection and efficacy as a stand-alone procedure.

Background and Objectives: AGEs, via RAGE, increase the development of hyperglycemia-induced liver damage, and blocking this axis is associated with a reduction in liver disease progression. The goal of this study was to determine how crocin and losartan influenced RAGE, TNF-α and TGF-β gene expression in diabetic rats, as well as histological changes in liver tissue., Materials and Methods: Diabetes was induced in 40 male Wistar rats using Streptozotocin (50 mg/kg, IP). There were five groups of rats: diabetic and healthy groups, diabetic rats given crocin (50 mg/kg), losartan (25 mg/kg) and both (crocin + Los). Serum glucose, ALT and AST levels were measured 4 weeks later. qPCR was used to examine the TNF-α, TGF-β and RAGE gene expression in liver tissue., Results: Crocin was found to be effective in lowering FBG in the diabetes group. The serum levels of ALT and AST decreased in all treated groups, but this decrease was significant in the crocin + Los group (p < 0.05). The relative expression of RAGE, TNF-α and TGF-β genes was significantly higher in the diabetes group compared to the healthy group. The expression of these genes decreased in groups treated with crocin and Losartan compared to the diabetes group. The highest reduction in RAGE and TGF-β gene expression was reported in those treated with crocin + Los. Histopathology results showed that the diabetes group had more bile ducts and necrosis than the healthy control group, which had no tissue changes. Hepatocyte degeneration, bile duct proliferation, inflammatory changes and hepatocyte necrosis were mild in the treated groups, but no hepatocyte necrosis was observed in the crocin + Los group., Conclusion: Crocin may be a feasible therapeutic agent for treating diabetes and its symptoms when combined with pharmaceutical medications. Human research is still needed to reach clear conclusions., (© 2024 The Author(s). Endocrinology, Diabetes & Metabolism published by John Wiley & Sons Ltd.)

Flaviviruses transmitted by arthropods, including the Murray Valley Encephalitis Virus (MVEV), are RNA viruses capable of causing severe encephalitis in various hosts. The spread of these viruses is closely linked to climatic conditions and the habitats of host and vector species, leading to outbreaks in new geographic regions. Notable encephalitis-causing flaviviruses include Japanese encephalitis virus (JEV), West Nile virus (WNV), and Kunjin virus (KUNV). MVEV, primarily spread by the mosquito Culex annulirostris and amplified by water birds such as egrets and Nankeen night herons, has caused significant outbreaks in Australia, including severe epidemics in 1951, 1956, and 1974. Despite its severity, no rapid diagnostic techniques or effective antiviral treatments are available, and current interventions are limited to supportive care and mosquito management. Given the absence of a licensed vaccine, this study aimed to develop a multi-epitope hybrid vaccine targeting MVEV using in silico approaches. The study focused on identifying B-cell and T-cell epitopes from the MVEV Envelope (E) protein, constructing a vaccine candidate, and computationally validating its immunogenic potential. The designed vaccine underwent rigorous analysis of its antigenic properties, allergenicity, and toxicity. Disulfide engineering and assessment of physicochemical properties ensured the structural integrity of the vaccine, supported by Ramachandran plot and ProSA web analyses. Molecular docking studies assessed the vaccine's binding affinities with TLR-3, and MHC-I. Population coverage analysis of MHC-I and MHC-II epitopes evaluated global efficacy. Additionally, molecular dynamics simulations explored the stability of docked complexes, and PDBsum analysis elucidated interaction details. Immunological simulations were conducted to predict immune response outcomes, providing comprehensive validation of the vaccine's antigenicity. The findings highlight the potential of a multi-epitope vaccine as a viable strategy for MVEV prevention., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Hyperlipidemia is a key risk factor mainly for hypertension and cardiac abnormalities. Previously eucalyptus plant (river red gum) had been used for its medicinal value for the treatment of many ailments. This study focused on phytochemical examination, investigation of an in vitro potential and in vivo effects in mice fed with high cholesterol diet, GC-MS analysis of extracts of Eucalyptus camaldulensis leaves and further confirmation of anti-hyperlipidemic potential of different constituents of plant extracts by using in silico technique. For in vitro study screening of different extracts of Eucalyptus camaldulensis leaves was performed by using pancreatic lipase enzyme inhibition assay. Ethanolic extract presented the highest potential among all the extracts by inhibiting pancreatic lipase having IC 50 -11.88 µg/mL. For in vivo study mice were fed with high cholesterol diet for induction of Hyperlipidemia. Water extract showed great anti-hyperlipidemic potential by reducing the level of cholesterol, triglycerides, low density lipoproteins and increasing high density lipoproteins level significantly ( p  < 0.05). Moreover, molecular docking and prime MM-GBSA study were applied for screening of compounds having anti-hyperlipidemic potential which showed that Alpha-cadinol was the lead compound for inhibition of pancreatic lipase enzyme having docking score (-6.604). The ADMET properties and toxicity profile of the top docked compounds were also detailed for ensuring their safety aspects. In this way in silico analysis substantiate the experimental findings by showing anti-hyperlipidemic potential in constituents of eucalyptus plant. Thus, there is a need of advanced research for isolation of active constituents having said anti-hyperlipidemic potential in the Eucalyptus camaldulensis plant.Communicated by Ramaswamy H. Sarma.

Ferroptosis, a form of iron-dependent regulated cell death, is characterized by the accumulation of lipid peroxides and distinctive morphological features. Moreover, the reduction of intracellular antioxidant enzyme expression or activity, specifically glutathione peroxidase 4 (GPX4) results in activation of the endogenous pathway of ferroptosis. In this review, we aimed to explore the intricate interplay between microRNAs (miRNAs) and ferroptosis, shedding light on its implications in various disease pathologies. This review delves into the role of miRNAs in modulating key regulators of ferroptosis, including genes involved in iron metabolism, lipid peroxidation, and antioxidant defenses. Furthermore, the potential of targeting miRNAs for therapeutic interventions in ferroptosis-related diseases, such as cancer, neurodegenerative disorders, and ischemia/reperfusion injury, is highlighted., Competing Interests: Declaration of competing interest The authors declare that they have no conflicts of interest., (Copyright © 2024. Published by Elsevier Inc.)

Faced with anomalies in medical images, Deep learning is facing major challenges in detecting, diagnosing, and classifying the various pathologies that can be treated via medical imaging. The main challenges encountered are mainly due to the imbalance and variability of the data, as well as its complexity. The detection and classification of skin diseases is one such challenge that researchers are trying to overcome, as these anomalies present great variability in terms of appearance, texture, color, and localization, which sometimes makes them difficult to identify accurately and quickly, particularly by doctors, or by the various Deep Learning techniques on offer. In this study, an innovative and robust hybrid architecture is unveiled, underscoring the symbiotic potential of wavelet decomposition in conjunction with EfficientNet models. This approach integrates wavelet transformations with an EfficientNet backbone and incorporates advanced data augmentation, loss function, and optimization strategies. The model tested on the publicly accessible HAM10000 and ISIC2017 datasets has achieved an accuracy rate of 94.7%, and 92.2% respectively., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)

The recent high-energy density sodium (Na) metal batteries (SMBs) are restricted by their processability, lifetime, and safety. These issues can be addressed by controlling the reactions at the Na metal by modifying the Na metal anode (SMA) with the sodiophilic hosts. Herein, a multifunctional Na 2 Se/Zn-Mn skeleton is introduced for SMA and fabricate a processable Na@Na 2 Se/Zn-Mn composite using repeated cold rolling/folding approach through the spontaneous reaction between Na metal and ZnMnSe alloy. This unique intermetallic composite has Zn and Mn metal sites for uniform deposition of Na, while Na 2 Se generates a stable SEI for rapid Na + ion transport. It offers outstanding sodiophilicity, high processability, excellent mechanical strength, and high ionic conductivity due to the synergistic effect of multi-component, enabling stable Na plating/stripping at high current densities and areal capacities. An optimized Na 2 Se/Zn-Mn skeleton enables Na||Na symmetric cells to attain a high critical current density of 5.0 mA cm -2 at 5.0 mAh cm -2 with an extended lifespan of 9000 h at 1.0 mA cm -2 at 1.0 mAh cm -2 . Remarkably, when configured into a full cell with a Na 3 V 2 (PO 4 ) 3 cathode, the SMB displays an extended lifespan of 2000 cycles at 10 C and an impressive high-rate capacity of ≈61.6 mAh g - ¹ at 30 C., (© 2024 Wiley‐VCH GmbH.)

Background: Multiple Sclerosis (MS) is a complex and chronic autoimmune disease that affects the central nervous system. Inflammation and demyelination characterize it, which results in a range of neurological impairments. The increasing worldwide occurrence of MS, affecting an estimated 2.8 million individuals in 2020, highlights the urgent requirement for further research to tackle the significant impact it has on individuals and healthcare systems globally., Objective: In this study, we wanted to explore the complex function of the endoplasmic reticulum (ER) in the origin, development, and resolution of MS, emphasizing its importance in neuroinflammatory illnesses. The ER has become a central focus in comprehending the pathogenesis of MS. Upon reviewing the literature, we observed a lack of thorough analysis that explores the involvement of endoplasmic reticulum stress in multiple sclerosis. Thus, we aimed through this research to examine the correlations between ER stress and its influence on immunological dysregulation, demyelination, and neurodegeneration in MS., Findings: Based on the latest clinical trials, we suggested theories that explore possible biomarkers linked to ER stress and the unfolded protein response. Identifying molecules that are suggestive of early stages of illness and can serve as prognostic tools for improving our understanding of the heterogeneity of MS and offering novel approaches for managing the disease. Finally, through our comprehensive search, we wanted to offer a plan for future research, suggesting new and creative methods for managing MS and encouraging the creation of specific treatments that aim to reduce the impact of MS on individuals worldwide., Competing Interests: Declarations. Ethical approval: No ethics committee approval was necessary based on the nature of the manuscript. Consent to participate: NA. Consent to publish: NA Conflict of interest: All authors declare no conflict of interest., (© 2024. Fondazione Società Italiana di Neurologia.)

Background: Nuclear medicine diagnostic and treatment procedures represent significant sources of ionizing radiation exposure for both staff and patients. Consequently, assessing and optimizing radiation doses are crucial to minimize potential side effects., Aim: This study seeks to evaluate the effective radiation doses associated with common diagnostic and treatment procedures, as well as propose diagnostic reference levels (DRLs), within two nuclear medicine centers in Madinah, Saudi Arabia., Methodology: Data from 445 patients were gathered from two nuclear medicine centers in the Madinah region of Saudi Arabia. The data were categorized based on the type of nuclear medicine (NM) procedure, the chemical composition of the administered radiopharmaceutical, as well as patient age and weight. Effective radiation doses for prevalent NM procedures were computed, and suggested DRLs were formulated., Results: Effective radiation doses were analyzed for 16 adult and 2 pediatric NM procedures (divided into 8 groups). The effective radiation doses for adult diagnostic nuclear medicine procedures range from 0.05 mSv (Nanocoloid) to 29 mSv (67Ga-citrate). For pediatric procedures, the doses range from 0.80 mSv (5-year-old undergoing renal DTPA) to 1.6 mSv (1-year-old undergoing renal DMSA). Furthermore, DRL values were determined for both adult and pediatric NM procedures. The study's findings demonstrated a high degree of concordance between effective radiation doses and DRL values, aligning well with previously published research., Conclusion: While the effective radiation doses outlined in this study were generally within acceptable limits and consistent with prior research findings, optimizing radiation doses remains imperative, particularly for pediatric NM procedures., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

In the present research, we investigate Congo red (CR) removal by layered double hydroxide and oxide AlCa on cellulose acetate (CA) fiber as anion-adsorbents in aqueous solution. The as-prepared composite was characterized by FE-SEM, XRD, FTIR, EDS-mapping and BET-BJH analyses. The CR adsorption ability on AlCa LDH/CA and AlCa LDO/CA adsorbents was evaluated. The removal property, dye adsorption and filtration properties of the AlCa LDO/CA composite were studied for removal CR based on central composite design (CCD) technique through investigating operational variables (temperature, adsorbent dosage, pH and contact time). The fabricated AlCa LDO/CA composite indicates a high removal efficiency up to 98.7 % for the CR removal in the 16 min. The data of the adsorption equilibrium were described by the Langmuir, Freundlich, Temkin and Dubinin-Radushkevich isotherms, and exhibited that AlCa LDH/CA fibers and AlCa LDO/CA fibers followed a pseudo-second-order kinetic model and Langmuir isotherm. The stability of Al-Ca-LDO/CA fibers nanocomposite was indicated that it was >95 % after eight cycles for removal of CR in the batch method on stirrer. The findings illustrated that appropriate AlCa LDO/CA fiber could be an efficient technique for CR elimination., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Patients with abdominal symptoms are among the most common to present both urgently and non-urgently for medical evaluation. While intestinal ultrasound (IUS) is useful to aid management of these patients, it is not routinely taught, performed, or included as part of the core set of point-of-care ultrasound competencies. The authors present an approach to performing a systematic, but focused, IUS at the bedside and diagnostic accuracies for small bowel obstruction inflammatory bowel disease, appendicitis, and diverticulitis. An approach on how to integrate IUS findings and common pitfalls will also be discussed., Competing Interests: Disclosure K. Piro receives nominal consulting fees from Exo for product feedback. I.W.Y. Ma holds a research chair at the University of Calgary and serves on the Advisory Board on the American College of Physicians and American Institute of Ultrasound in Medicine. She does not receive personal payment for any of these roles. K. Novak reports advisory board fees from AbbVie, Janssen, Takeda, Pfizer, Ferring, Lily, Celltrion; speaker’s fees from AbbVie, Janssen, Pfizer, Fresnius Kabi; Pfizer and Janssen are global entities - it is their Canadian branch that has provided education support (both in grants to do US research or consensus)., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Objective:  Defective Goblet cells have been proposed to be involved in necrotizing enterocolitis (NEC). The aim was to study the expression of the Goblet cell marker REG4 and its potential involvement in NEC in preterm infants with and without NEC., Study Design:  Seventy histologically intact intestinal biopsies were studied: 43 were collected during surgery due to NEC (NEC group: 26.5 ± 3.0 weeks' gestational age [wGA]), and 27 from individuals who underwent surgery due to other conditions (Control group; 36.1 ± 4.5 wGA). The tissue samples were immunohistochemically stained for REG4. REG4 expression was quantified with a semiautomated digital image analysis and with clinical data compared between the groups., Results:  REG4 expression was lower in the NEC group than in the Control group ( p  = 0.035). Low REG4 expression correlated to the risk of NEC ( p  = 0.023). In a multivariable logistic regression analysis including GA and REG4 expression for NEC risk, only GA ( p  < 0.001) and not REG4 expression ( p  = 0.206) was associated with NEC risk., Conclusion:  This study concludes that Goblet cell dysfunction may be involved in NEC development, as low expression of the Goblet cell marker REG4 was related to an increased NEC risk in preterm infants. Maturity could however not be excluded as a potential confounder for REG4 expression., Key Points: · REG4 is a specific Goblet cell marker not yet studied in NEC.. · REG4 was quantified in intestinal biopsies from infants with and without NEC.. · REG4 expression was lower in infants with NEC, and expression seems to be maturity dependent.., Competing Interests: None declared., (Thieme. All rights reserved.)

Objective: Identifying connections between various aspects of physical performance, motor skills, and cognitive abilities with the brain connectivity networks is essential for determining important brain regions associated with soccer performance. This study aimed to carry out the relationship between soccer-specific parameters and resting-state functional connectivity in soccer players., Materials and Methods: Twenty-five soccer players (Vo2max; 50.68 ± 3.76 ml/min/kg) were participated voluntarily. The study encompassed a comprehensive assessment, including measures of physical performance, skill performance, and executive function (design fluency). Each participants underwent resting-state functional magnetic resonance imaging (fMRI) also allowing for the acquisition of data. The Linear regression were conducted between sport-specific parameters and functional connectivity values., Results: Our findings revealed a significant positive correlation between agility and linear sprinting with the dorsal attention network (DAN). Conversely, the aerobic fitness parameter demonstrated exhibited a significant negative correlation with the DMN. Skill performance (speed dribbling, passing, shooting) displayed a significant positive correlation with both sensorimotor network (SMN), and DAN. Moreover, design fluency test displayed a significant positive correlation with the DMN. Notably, IPSL and IPSR nodes within the DAN consistently demonstrated the highest degree of centrality across various sports parameters., Conclusion: This study provides valuable insights into the intricate relationship among physical performance, skill proficiency, and executive function, as they relate to the functional connectivity of specific neural networks in soccer players. The outcomes establish a foundational understanding for future exploration and potential applications in sports science, and cognitive neuroscience in order to improve soccer performance., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2024. Published by Elsevier Ltd.)

Introduction: Glipizide is an oral antidiabetic drug widely used to treat non-insulin-dependent type II diabetes mellitus (NIDDM). This systematic review extensively examines all reported pharmacokinetic (PK) parameters of glipizide in healthy and diseased populations., Areas Covered: A total of 31 articles were retrieved after screening various databases, i.e. Google Scholar, PubMed, Science Direct, and Cochrane, regarding the PK parameters of glipizide in healthy, diseased, drug-drug, and drug-food interaction studies. The C max was 35% higher in healthy Koreans than in Caucasian Americans. In type II diabetes patients, the AUC 0-∞ increases ~2-fold after multiple dosage regimen in comparison with a single dose. Furthermore, the C max increased in fasting conditions compared to the non-fasting state in diabetic individuals i.e. 1338.28 ± 125.18 ng/mL and 1297.29 ± 47.22 ng/mL, respectively., Expert Opinion: The presented data has depicted that glipizide exposure varies between single and multiple dosing and its C max also changes between different demographic populations. Since it has a shorter half-life, the development of its new extended-release formulations may assist practitioners in improving adherence among diabetic patients., Prospero Registration No: CRD42024538428.

Background: Poly (β-amino Ester) nanocarriers show promise for gene therapy, but their effectiveness can be limited by the environment within the body. This study aims to understand how common cell culture media components affect optimized PBAE nanocarrier performance in gene delivery., Methods: Optimized PBAE was synthesized based on Michael addition reaction and characterized by different assays, this study employed techniques like DLS and TEM to characterize PBAE nanocarriers, followed by cellular uptake analysis (flow cytometry and confocal imaging) and evaluation of gene expression under different polymer/DNA ratio ratios and media conditions., Results: The nanocarriers exhibited size under 200 nm and surface positive charge, with high encapsulation efficiency (up to 95%). Cellular uptake, transfection efficiency, and cytotoxicity were evaluated. Flow cytometry analysis revealed high cellular uptake (over 77% at 1 hour and up to 95% after 3 hours) and good viability. Transfection efficiency reached up to 80% with 2 μg DNA, particularly at weight ratios of 60 and 90., Conclusion: The study also identified factors affecting transfection efficiency, including serum concentration and antibiotics in the culture medium, highlighting the importance of optimizing these conditions for future applications.

Background: Early and reliable prognostication in post-cardiac arrest patients remains challenging, with various factors linked to return of spontaneous circulation (ROSC), survival, and neurological results. Machine learning and deep learning models show promise in improving these predictions. This systematic review and meta-analysis evaluates how effective these approaches are in predicting clinical outcomes at different time points using structured data., Methods: This study followed PRISMA guidelines, involving a comprehensive search across PubMed, Scopus, and Web of Science databases until March 2024. Studies aimed at predicting ROSC, survival (or mortality), and neurological outcomes after cardiac arrest through the application of machine learning or deep learning techniques with structured data were included. Data extraction followed the guidelines of the CHARMS checklist, and the bias risk was evaluated using PROBAST tool. Models reporting the AUC metric with 95 % confidence intervals were incorporated into the quantitative synthesis and meta-analysis., Results: After extracting 2,753 initial records, 41 studies met the inclusion criteria, yielding 97 machine learning and 16 deep learning models. The pooled AUC for predicting favorable neurological outcomes (CPC 1 or 2) at hospital discharge was 0.871 (95 % CI: 0.813 - 0.928) for machine learning models and 0.877 (95 % CI: 0.831-0.924) across deep learning algorithms. For survival prediction, this value was found to be 0.837 (95 % CI: 0.757-0.916). Considerable heterogeneity and high risk of bias were observed, mainly attributable to inadequate management of missing data and the absence of calibration plots. Most studies focused on pre-hospital factors, with age, sex, and initial arrest rhythm being the most frequent features., Conclusion: Predictive models utilizing AI-based approaches, including machine and deep learning models exhibit enhanced effectiveness compared to previous regression algorithms, but significant heterogeneity and high risk of bias limit their dependability. Evaluating state-of-the-art deep learning models tailored for tabular data and their clinical generalizability can enhance outcome prediction after cardiac arrest., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Purpose: This study examined the augmented reality (AR) application in design evaluations through an immersive experience of medical task simulations in combination with a full-scale physical mock-up of a trauma room., Background: Augmented reality technology is emerging in various fields including architectural design. Traditionally, building physical mock-ups has been the most effective tool to involve end-users in design evaluations. However, AR can eliminate cost- and labor-intensive components of a physical mock-up by replacing them with holograms., Methods: Two simulation sessions with emergency department clinicians were conducted using AR. The research team collected user feedback through simulations and interviews. Clinicians' feedback was systematically categorized and summarized according to various design elements., Result: The integration of mock-up and AR simulations enabled the evaluation of design elements that were previously unattainable. Examples include simulating color-coded floor boundaries and adjusting door widths, which enhanced clinicians' immersion and involvement in design changes. Interviews and analyses of video recordings captured from the clinicians' perspectives supported the design evaluations during simulations., Conclusions: Augmented reality has great potential to be used widely in the evaluation of healthcare environment design. Augmented reality is an innovative approach that can benefit end-users in interacting with a mix of real spaces and virtual components. It also introduces new ways of data collection and analysis to architectural design evaluation methods., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.