Search

Abstract Background Identifying therapeutic inhibitors of crucial enzymes involved in the peptidoglycan biosynthesis pathway is pivotal for developing new treatments against multidrug-resistant Enterococcus faecalis V583. MurM, an essential enzyme in this pathway, plays a significant role in the bacterium’s cell wall synthesis, making it an attractive druggable target for novel antimicrobial strategies. This study explored the potential of natural compounds as inhibitors of MurM, aiming to discover promising drug candidates that could serve as the foundation for future therapeutic development. Methods The three-dimensional structure of MurM was predicted, optimized, and its binding pocket was analyzed by comparing it with related structures. Over 4,70,000 natural compounds from the COCONUT database were subjected to virtual high-throughput screening (vHTS). The top lead candidates were selected based on their Lipinski’s profile, ADME profile, toxicity profile, estimated binding free energy (ΔG) and estimated inhibition constant (Ki). Interaction pattern analysis was used to evaluate the non-covalent interactions between the inhibitors and key residues in MurM’s binding pocket. Molecular dynamics simulations were performed over 300 ns to assess the structural stability and impact of these inhibitors on MurM’s enzyme. Results Three lead compounds—CNP0056520, CNP0126952, and CNP0248480—were identified and prioritized with estimated ΔG ranging from − 9.35 to -7.9 kcal/mol. Molecular dynamics simulations revealed minimal impact on MurM’s overall structure and dynamics, with the candidate inhibitors forming stable protein-ligand complexes. These interactions were supported by several non-covalent interactions between the candidate inhibitors and key residues within MurM’s binding pocket. Conclusion These findings suggest that the identified natural product candidates could serve as promising inhibitors of MurM, potentially leading to novel therapeutics targeting cell wall biosynthesis in multidrug-resistant E. faecalis.

By leveraging the properties of natural or plant fibers and possibilities through three-dimensional (3D) printing technology, a composite filament was fabricated by incorporating newly isolated Cryptostegia grandiflora fiber (CGF), as a reinforcement with polylactic acid (PLA) by using a twin-screw extruder. The fabricated composite filament and pure PLA filament were 3D-printed, using fused deposition modeling (FDM). This study investigated the mechanical, physical, and thermal properties of the 3D-printed CGF reinforced composite filament samples. The mechanical properties of the samples fabricated with 10 wt% CGF were better than that of samples with pure PLA. In addition, impact, tensile, flexural strengths and hardness were increased by 35.6, 33.6, 14.1, and 1.7%, respectively, when compared with the sample with pure PLA. The fractured surface morphology of tensile samples showed a uniform distribution of CGF within the PLA. The addition of CGF improved the thermal stability of the 3D-printed CGF/PLA composite sample by 15%. Therefore, the printed structure could serve as an alternative material for various uses, considering contemporary concepts of sustainability, availability, environmental friendliness, and cost effectiveness.

Acrylonitrile butadiene styrene (ABS) polymer and carbon fiber reinforced acrylonitrile butadiene styrene (CF/ABS) spur gears were 3D-printed using fusion deposition modeling (FDM) with different fillet radii of 0.25, 0.50, and 0.75 mm. The performance of the fabricated gears was studied with the effect of fillet radius on varying load and speed conditions. The thermal properties of the gears were also investigated. The results indicated that 3D-printed CF/ABS spur gear exhibited better performance than the pure ABS. The 3D-printed CF/ABS gear with fillet radius of 0.25 mm recorded the highest wear and thermal stresses. However, the optimum performance was exhibited by the gear sample with highest fillet radius of 0.75 mm. Repeated gear tooth loading during service caused an increase in gear temperature due to the hysteresis and friction. Using optical microscopy, the tooth structures of both 3D-printed ABS and CF/ABS spur gears were analyzed before and after loading conditions to establish their failure mechanism. Evidently, various applications of the FDM 3D-printed spur gears depend on their different performances under loads and operating speeds. The methods and findings of this work can be regarded as helpful for future related work related to cellulosic reinforcing particles in a polymer matrix.

Acrylonitrile butadiene styrene (ABS) polymer and carbon fiber reinforced acrylonitrile butadiene styrene (CF/ABS) spur gears were 3D-printed using fusion deposition modeling (FDM) with different fillet radii of 0.25, 0.50, and 0.75 mm. The performance of the fabricated gears was studied with the effect of fillet radius on varying load and speed conditions. The thermal properties of the gears were also investigated. The results indicated that 3D-printed CF/ABS spur gear exhibited better performance than the pure ABS. The 3D-printed CF/ABS gear with fillet radius of 0.25 mm recorded the highest wear and thermal stresses. However, the optimum performance was exhibited by the gear sample with highest fillet radius of 0.75 mm. Repeated gear tooth loading during service caused an increase in gear temperature due to the hysteresis and friction. Using optical microscopy, the tooth structures of both 3D-printed ABS and CF/ABS spur gears were analyzed before and after loading conditions to establish their failure mechanism. Evidently, various applications of the FDM 3D-printed spur gears depend on their different performances under loads and operating speeds. The methods and findings of this work can be regarded as helpful for future related work related to cellulosic reinforcing particles in a polymer matrix.

Biochar production from unconventional biomass, specifically onion peel (OP) and chicken feathers (CF), was investigated in this study. Two distinct biochars were produced by doping each biomass with the other, with the aim of exploring the synergistic effects of different feedstock combinations on biochar properties. The biochar production process was conducted using a retort heating method and characterized using several techniques. A yield of 36 % was obtained for OP-doped biochar (OP92CF8-BC) and 23 % for CF-doped biochar (F92OP8-BC). Fourier Transform Infrared Spectroscopy analysis revealed characteristic functional groups from cellulose, hemicellulose, and lignin in OP92CF8-BC, while CF92OP8-BC displayed keratin-related peaks. Scanning Electron Microscopy imaging showed surface morphology differences, with OP92CF8-BC exhibiting a rougher and more porous structure compared to CF92OP8-BC. Energy-Dispersive X-ray Spectroscopy analysis confirmed the elemental composition, with OP92CF8-BC having higher carbon, calcium, and sulfur contents and CF92OP8-BC having higher nitrogen and oxygen contents. The biochar had specific surface areas of 342.4 and 200.80 m2/g for OP92CF8-BC and CF92OP8-BC, respectively. According to the results, using biochar treatments-more especially, CF92OP8-BC-can significantly enhance cob weight. This could be good for agricultural productivity. These findings highlight the influence of feedstock composition on the properties of biochar and provide insights for its potential applications in soil amendment and pollutant removal.

Lectins, glycoproteins with non-enzymatic and non-immunogenic origins, play essential roles in various biological processes. This study presents the purification and characterization of a lectin-like protein isolated from the seeds of Datura stramonium L., a member of the Solanaceae family. The purification of the lectin-like protein involved a dual method, employing both ammonium sulphate precipitation and anion exchange chromatography utilizing DEAE-Sepharose. Analysis via SDS-PAGE showed a distinct band, displaying an estimated molecular weight of approximately 30 kDa., indicative of the purified protein's homogeneity. Additionally, the purified protein exhibited hemagglutination activity towards human B and O red blood cells, underscoring its lectin-like properties. Periodic acid Schiff (PAS) staining confirmed the glycoprotein nature of the purified protein. Furthermore, its anti-proliferative activity against human cancer Caco-2 cell lines was evaluated through MTT assay, revealing promising results. This study elucidates the purification and characterization of a lectin-like protein from Datura stramonium L. seeds, providing valuable insights into its potential biological functions and diagnostic research applications.

Increasing prerequisites for sustainable energy storage and conversion technologies have necessitated the exploration of advanced materials with improved properties. Here, we present the synthesis and characterization of ZnO nanoparticles (NPs), ZnO/NiO, ZnO/Co3O4 and ZnO/NiO/Co3O4 NCs as promising bi-functional materials for dye-sensitized solar cell (DSSC) and electrochemical energy storage applications. A facile chemical co-precipitation approach was followed to synthesize pure ZnO NPs, ZnO/NiO, ZnO/Co3O4 and ZnO/NiO/Co3O4 NCs. The structural and morphological analyses revealed the successful integration of ZnO, NiO, and Co3O4 NPs and also the formation of well-defined core-shell and homogenous nanocomposite structures. The XRD and HRTEM analyses confirmed the crystalline nature and nanoscale morphology of synthesized materials, respectively. The photovoltaic performance of DSSC fabricated using ternary ZnO/NiO/Co3O4 NC photoanode showed optimum dye-loading and best solar to electrical energy conversion efficiency with Jsc of 11.29 mA cm−2 and ƞ of 4.66%, which was considerably higher than the DSSC fabricated using pure ZnO NPs photoanode (ƞ = 2.01%). The increment in photocurrent density (Jsc) could be ascribed to the perfect band alignment of NiO and Co3O4 NPs in the ternary NC. Further, the ZnO/NiO/Co3O4 NC photoanode integrated DSSC disclosed 97% retainment in energy conversion efficiency even after 10 days of operation. The electrochemical performance of supercapacitor fabricated using ternary ZnO/NiO/Co3O4 NC showed high specific capacitance of 534.7 Fg−1 at 1 Ag−1 with favourable rate ability (∼52% at 16 Ag−1), good cyclic stability (91.07%) and low internal resistance. Moreover, the ZnO/NiO/Co3O4 NC at a current density of 2 Ag−1 exhibited a significantly high capacitance value of 463.1 Fg−1, which was 1.93, 1.58 and 1.22 times greater than ZnO NPs, ZnO/Co3O4 NC and ZnO/NiO NC, respectively.

This research introduces a novel approach to repurposing walnut shells, an abundant agricultural waste, to synthesize sustainable nitrogen-doped activated carbon (N@AC). The resulting material exhibits remarkable properties suitable for dual applications in high-performance all-solid-state supercapacitors and efficient Rhodamine B dye (RhB) adsorption. In a three-electrode setup, the N@AC electrode exhibits an impressive specific capacitance of 484.6 Fg−1 at 1 Ag−1 and remarkable long-term stability, maintaining 97.4% of its initial performance even after 5000 charge-discharge cycles. Simultaneously, the all-solid-state symmetric supercapacitor configuration (N@AC//N@AC) demonstrates outstanding specific capacitance, registering at 168.8 Fg−1 at 1 Ag−1, accompanied by a favourable rate capability of 67.3% at 10 Ag−1. Notably, the N@AC//N@AC configuration attains a high energy density of 39.8 WhKg−1 at 1 Ag−1. Furthermore, N@AC//N@AC exhibits favourable cyclic stability, retaining 83.91% of its initial capacitance even after 10,000 charge-discharge cycles. Moreover, the adsorption efficiency of N@AC toward RhB is scrutinized, highlighting its efficacy in addressing environmental remediation challenges. The porous architecture and nitrogen functionalities of N@AC play a crucial role in expeditiously eliminating organic pollutants from aqueous solutions, offering a sustainable approach to treating wastewater. Optimal conditions for the highest RhB adsorption are identified: pH 7.2, a contact duration of 180 min, and an initial dye concentration of 20 mgL−1. Thermodynamic evaluations, encompassing the determination of ΔH◦, ΔH◦, and ΔS◦, signify the endothermic and spontaneous nature of the adsorption process. In desorption investigations, it is noted that H2O, employed as an eluting agent, proficiently releases 87.35% of the adsorbed RhB dye.

Both developed and developing countries around the world are increasingly utilizing biodegradable products and bio-based materials. This is required to curb rampant environmental pollution caused by synthetic materials and their by-products. In this study, banana and bamboo fibers were prepared from agricultural and industrial wastes, respectively. Banana and bamboo fibers were obtained with aid of mechanical and waste extractions, respectively. Both fibers were subjected to a retting process for 24 hours, using normal warm water at a room temperature (27 ± 3 °C) to remove the impurities. Then, a comparative investigation and analysis was conducted concerning their properties and applications. The biomass level, physical, and chemical properties, structure, experimental analysis, and moisture regain behaviors of the plant materials were studied. Additionally, the antibacterial property of the samples was discussed. The biomass level was measured per hectare for banana (36.1 tons) and per plant for bamboo (65%), and the physical and chemical properties were identified via some basic testing techniques. The molecular, crystalline, and morphology structures were observed using Fourier-transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. Finally, the industrial applications were elucidated to establish the possibility of using both fibers as promising sustainable, renewable, recyclable, and eco-friendly materials.

Both developed and developing countries around the world are increasingly utilizing biodegradable products and bio-based materials. This is required to curb rampant environmental pollution caused by synthetic materials and their by-products. In this study, banana and bamboo fibers were prepared from agricultural and industrial wastes, respectively. Banana and bamboo fibers were obtained with aid of mechanical and waste extractions, respectively. Both fibers were subjected to a retting process for 24 hours, using normal warm water at a room temperature (27 ± 3 °C) to remove the impurities. Then, a comparative investigation and analysis was conducted concerning their properties and applications. The biomass level, physical, and chemical properties, structure, experimental analysis, and moisture regain behaviors of the plant materials were studied. Additionally, the antibacterial property of the samples was discussed. The biomass level was measured per hectare for banana (36.1 tons) and per plant for bamboo (65%), and the physical and chemical properties were identified via some basic testing techniques. The molecular, crystalline, and morphology structures were observed using Fourier-transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. Finally, the industrial applications were elucidated to establish the possibility of using both fibers as promising sustainable, renewable, recyclable, and eco-friendly materials.

At present, composites have brought materials to a new era with superior characteristics. The proper selection of materials with optimal processing conditions is one of the prime factors in determining the efficiency of composites. This article introduces a new composite by fabricating a copper matrix with reinforced AlCoCrCuFe High Entropy Alloy (HEA). The ultimate theme of this research is to forecast the wear rate of the Cu–AlCoCrCuFe HEA composite. Using the pin on the disk, the wear test is carried out on varying combinations of parameters. Then Taguchi analysis is carried out to get the precise fit parameters. The wear rate mainly depends on the percentage of HEA, sliding distance, sliding velocity, and the load applied. Correlation analysis is performed to determine the exact parameter determining the optimal wear rate and the coefficient of friction. After feature extraction, the parameters are optimized, and the neural network regression is given the optimized parameters. The network has been trained, and predictions are made using it. The model successfully predicts the wear rate, as evidenced by the declining RMSE of 0.28 and rising R2 values up to 92%. The analysis shows a significant decline in the wear rate with HEA additions. In addition, the wear rate increases with a rise in load, sliding velocity, and sliding distance.

Warfarin is a cardiovascular drug, used to treat or inhibit the coagulation of the blood. In this paper, we have studied the interaction of lysozyme with warfarin using several experimental (fluorescence, UV–visible and circular dichroism spectroscopies) and computational (molecular docking, molecular dynamics and DFT) approaches. Experimental studies have suggested that there was a strong interaction between lysozyme and warfarin. Inner filter effect played important role in fluorescence experimental data which show that the emission intensity of lysozyme decreased on the addition of warfarin, however, after inner filter effect correction the actual outcome turned out be the fluorescence enhancement. The extent of binding, increased with temperature rise. The interaction was primarily taken place via the dominance of hydrophobic forces. Small amount of warfarin didn't influence the secondary structure of lysozyme; however, the higher concentration of warfarin caused a decrease in the helicity of the protein and a consequent partial unfolding. Molecular docking studies were also performed which revealed that warfarin binds with lysozyme mainly with hydrophobic forces along with a significant contribution of hydrogen bonding. The flexibility of warfarin played important role in fitting the molecule into the binding pocket of lysozyme. Frontier molecular orbitals of warfarin, using DFT, in free as well as complexed form have also been calculated and discussed. Molecular dynamics simulations of unbound and warfarin bound lysozyme reveal a stable complex with slightly higher RMSD values in the presence of warfarin. Despite slightly increased RMSF values, the overall compactness and folding properties remain consistent, emphasizing strong binding towards lysozyme through the results obtained from intermolecular hydrogen bonding analysis. Essential dynamics analysis suggests warfarin induces slight structural changes without significantly altering the conformation, additionally supported by SASA patterns. Aside from the examination of global and essential motion, the MM/PBSA-based analysis of binding free energy elucidates the significant binding of warfarin to lysozyme, indicating a binding free energy of −13.3471 kcal/mol.

The current research investigates diesel and simarouba biodiesel blends (10%, 20%, & 30% by volume) in conventional and Low Heat Rejection (LHR) diesel engines, each rated at 4.4 kW. While optimization techniques like Response Surface Method and Taguchi have been extensively studied, the impact of LHR and optimization on LHR engine performance and emissions is rarely explored. Converting the conventional engine to LHR involved applying 300 μm of stabilized zirconia to the piston crown to enhance combustion efficiency. Performance and emissions were analyzed at rated injection pressure (200 bar) and timing (23° before top dead center - btdc). Experiments were continued on LHR engine by varying injection timings (advancing - 26°btdc and retarding - 20°btdc). Advanced injection timing showed significant improvement in performance of Low heat rejection engine. MINITAB statistical tool is used to optimize engine performance using Response Surface Method. The 20% blend showed improved performance in both engines. The optimum values for Low heat rejection engine responses are 26.8%, 0.32 kg/kW-h, 0.018%, 59.59 ppm, and 1419.03 ppm for brake thermal efficiency, brake specific fuel consumption, carbon monoxide, and unburnt hydrocarbons, respectively. Confirmation experiments aligned well with model predictions, indicating the potential of LHR engines to enhance thermal efficiency and reduce emissions.

Vehicle emissions are responsible for about 30% of all air pollution in the world. Vehicle emissions can be significantly reduced through the use of selective catalyst reduction (SCR). The present work emphasizes the impact of thermal barrier-coated pistons on diesel engine performance as well as emission qualities. A Ni–Cr–Al–Y bond coat was applied to the tested engine piston that was 50 microns thick and a top coat that was 250 microns thick. These coatings were applied using the plasma spray technique to a combination of 2 mol. % of Gadolinium oxide (Gd2O3), 2 mol. % of Neodymium oxide (Nd2O3), 3 mol. % of Yttria (Y2O3), and continuing 93 mol. % of Zirconia (ZrO2). In a 4-stroke, 1-cylinder diesel engine, the testing was carried out utilizing diesel, Mahua, and Jatropha fuels with and without coating. The selective catalytic reduction technique was employed in the current test to reduce NOx emissions. The findings of this analysis indicate that the brake thermal efficiency of an insulated piston engine improved by 3.9%, and when JB 100 was chosen as the fuel, the insulated piston reduced brake-specific fuel consumption by 3.5% in comparison to the normal piston. In engines coated with SCR, hydrocarbon emissions were lowered by 20.1%, while carbon monoxide emissions were dropped by 13.4%. In comparison to the baseline engine, the oxide of nitrogen emissions were reduced by 39.1%.

In this work, four varieties of hybrid Fiber-Reinforced Polymer (FRP) panels made of kevlar-29 and natural fibers are studied. All panels have kevlar-29 face sheets and natural fiber core, such as jute, flax, sisal, and hemp. This research focuses on the behavior of these hybrid FRP panels under flexural and impact loading so that the panels can be explored for the structural/semi-structural members of army shelters, portable helipad, and roofing panels in high-altitude areas. Natural fibers are chemically treated with NaOH to improve hydrophobicity. The panels are vacuum bagged, the fiber volume fraction is 0.39, and the thickness is close to 4 mm. Three-point flexural loading using the universal testing machine and low-velocity impact loading up to 24 J under drop weight impact test setup is carried out to characterize the panels. Damage area, delamination, permanent deformation, indentation depth, energy absorbed, flexural strength, and modulus are measured. The hybrid flax/kevlar panel and hemp/kevlar panel, each resist impact with permanent deformation less than 0.5 mm up to 24 J. Without significant face sheet or core fiber breakage, the delamination is spread over a small radial distance of 18.5 and 24.5 mm, respectively. Interface matrix breakage causes delamination. The load vs deflection curve is almost linear under flexural loading, and specimens failed under compression at 240 MPa. The numerical simulation is also done using ANSYS and LS-DYNA for detailed study.

Aluminum alloy and its allied composites are gaining popularity and find their exclusive application in all industries for more than two decades. Due to their increased usage, Al metal matrix composites were experimented by reinforcing them with variety of particles. The current study attempted the use of titanium carbide (TiC) and boron nitride (BN) as reinforcing materials for the Al7050 alloy through the stir casting technique. The percentage of reinforcement of TiC was kept constant as 8% by wt., and the BN was varied as 2% and 3% by wt. Mechanical properties, such as compressive strength, tensile strength, flexural strength, impact energy, microhardness (Vickers), the microstructure were studied using an optical microscope and by corrosion tests using polarization techniques. The results conclude that the Al hybrid composite with 8% TiC and 3% BN provides superior performance.

Medical life-saving techniques include mechanical ventilation. During the COVID-19 epidemic, the lack of inexpensive, precise, and accessible mechanical ventilation equipment was the biggest challenge. The global need exploded, especially in developing nations. Global researchers and engineers are developing inexpensive, portable medical ventilators. A simpler mechanical ventilator system with a realistic lungs model is simulated in this work. A systematic ventilation study is done using the dynamic simulation of the model. Simulation findings of various medical disorders are compared to standard data. The maximum lung pressure (Pmax) was 15.78 cmH2O for healthy lungs, 17.72 for cardiogenic pulmonary edema, 16.05 for pneumonia, 19.74 for acute respiratory distress syndrome (ARDS), 17.1 for AECOPD, 19.64 for asthma, and 15.09 for acute intracranial illnesses and head traumas. All were below 30 cmH2O, the average maximum pressure. The computed maximum tidal volume (TDVmax) is 0.5849 l, substantially lower than that of the healthy lungs (0.700 l). The pneumonia measurement was 0.4256 l, substantially lower than the typical 0.798 l. TDVmax was 0.3333 l for ARDS, lower than the usual 0.497 l. The computed TDVmax for AECOPD was 0.6084 l, lower than the normal 0.700 l. Asthma had a TDVmax of 0.4729 l, lower than the typical 0.798 l. In individuals with acute cerebral diseases and head traumas, TDVmax is 0.3511 l, lower than the typical 0.700 l. The results show the viability of the model as it performs accurately to the presented medical condition parameters. Further clinical trials are needed to assess the safety and reliability of the simulation model.

Manufacturing has grown challenging because of the increased usage of harder materials, such as titanium alloys, in many industries, such as aerospace, automobiles, and marine. Conventional material removal procedures are not suitable for these tough materials due to their increased hardness and slow machinability. Wire Electrical discharge machining (WEDM) is a modern approach for material removal, particularly for harder materials, such as titanium alloys, nickel alloys, hard particle reinforced metal matrix composites, etc. The research design was performed by deeming the independent factors, such as duration of pulse and applied current. The removal rate of material, surface roughness of the machined region, dimensional deviation, and tolerance errors in form/orientation are considered performance metrics. Taguchi’s approach was engaged to assess the process variables, and the importance of the process factors was established using analysis of variance approach. The purpose of this research is to create an AI based decision making tool, which can be utilized to anticipate the various parameters that impact the WEDM material removal process. The discoveries of the present exploration allowing the manufacturers to make better-informed decisions with a developed model’s capability by demonstrating that the model’s predicted values were in close confirmation to the actual values.

Algerian kaolinite, sourced from Djebel Debbagh nuance 3 (DD3), was used as a low-cost adsorbent to remove methylene blue (MB) dye from water. Its adsorption capacity was enhanced through sulfuric acid treatment (treated-DD3). In response to the urgent demand for clean water, various technologies have been developed to address dye removal from wastewater. This study, specifically delving into the treatment of textile wastewater, examined the efficacy of treated-DD3 through adsorption processes. The acid treatment increased the surface area and pore volume of DD3. X-ray diffraction showed crystalline phases in both, with treated-DD3 having higher crystallinity. Fourier-transform infrared spectroscopy found no significant differences post-acid treatment. Scanning electron microscopy revealed DD3 had large, stacked particles with low surface area, while treated-DD3 had increased porosity and a smoother surface. Various parameters affecting MB adsorption were studied. The Langmuir and Freundlich models were used for isotherm parameters. Treated-DD3 exhibited a higher MB adsorption capacity (64.58 mg/g according to the Langmuir model) than DD3 (44.48 mg/g). Thermodynamic analysis indicated spontaneous and endothermic MB adsorption onto both DD3-BM and treated-DD3-BM systems under different pH conditions. Treated-DD3 effectively reduced chemical oxygen demand (from 304.056 mg/L to 34.44 mg/L) and biological oxygen demand (from 80 mg/L to 20 mg/L) in real textile wastewater. The adsorbent exhibited rapid removal and decolorization, surpassing 93% within the first 7 min of the experiment. The Gaussian process regression and particle swarm optimization (GPR–PSO) predicted MB adsorption capacity effectively (R = 0.9989, R2 = 0.9978, adj-R2 = 0.9978, RMSE = 1.1390, and MAE = 0.3926).