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Due to the black and glossy appearance of the carbon fiber prepreg bundle surface, the accurate identification of surface defects in automated fiber placement (AFP) presents a high level of difficulty. Currently, the enhanced YOLOv7 algorithm demonstrates certain performance advantages in this detection task, yet issues with missed detections, false alarms, and low confidence levels persist. Therefore, this study proposes an improved YOLOv7 algorithm to further enhance the performance and generalization of surface defect detection in AFP. Firstly, to enhance the model’s feature extraction capability, the BiFormer attention mechanism is introduced to make the model pay more attention to small target defects, thereby improving feature discriminability. Next, the AFPN structure is used to replace the PAFPN at the neck layer to strengthen feature fusion, preserve semantic information to a greater extent, and finely integrate multi-scale features. Finally, WIoU is adopted to replace CIoU as the bounding box regression loss function, making it more sensitive to small targets, enabling more accurate prediction of object bounding boxes, and enhancing the model’s detection accuracy and generalization capability. Through a series of ablation experiments, the improved YOLOv7 shows a 10.5% increase in mAP and a 14 FPS increase in frame rate, with a maximum detection speed of 35 m/min during the AFP process, meeting the requirements of online detection and thus being able to be applied to surface defect detection in AFP operations.

Adding methane to methanol can solve the problem of difficult cold starts of methanol engines. Therefore, it is important to understand the combustion of methane and methanol fuel blends. Because of this, this study explores the effect of methane addition on the laminar burning velocity and Markstein length of methanol/methane premixed flames under stoichiometric conditions at the initial temperatures of 353 K, 373 K, and 393 K, initial pressures of 1 bar, 2 bar, and 4 bar, using methane addition ratios of 0%, 25%, 50%, 75%, and 100% in a constant volume combustion chamber. The results show that the laminar burning velocity decreases linearly with the increase of methane addition ratio due to the linear decrease of the Arrhenius factor. The sensitivity analysis revealed that the kinetic effect is the main reason for the inhibition of laminar burning velocity, which is insensitive to initial temperature but enhanced at a high initial pressure. The Markstein length decreases with the addition of methane and the increase of initial pressure, which is mainly caused by the high mass diffusivity of methane and the decrease of flame thickness due to the increase of initial pressure. [ABSTRACT FROM AUTHOR]

Escherichia coli O157:H7 (E. coli O157) is known for causing severe foodborne illnesses such as hemorrhagic colitis and hemolytic uremic syndrome. Although E. coli O157 is typically regarded as an extracellular pathogen and a weak biofilm producer, some E. coli O157 strains, including a clinical strain ATCC 43895, exhibit a notable ability to invade bovine crypt cells and other epithelial cells, as well as to form robust biofilm. This invasive strain persists in the bovine host significantly longer than non-invasive strains. Various surface-associated factors, including lipopolysaccharides (LPS), flagella, and other adhesins, likely contribute to this enhanced invasiveness and biofilm formation. In this study, we constructed a series of LPS-core deletion mutations (waaI, waaG, waaF, and waaC) in E. coli O157 ATCC 43895, resulting in stepwise truncations of the LPS. This approach enabled us to investigate the effects on the biosynthesis of key surface factors, such as flagella and curli, and the ability of this invasive strain to invade host cells. We confirmed the LPS structure and found that all LPS-core mutants failed to form biofilms, highlighting the crucial role of core oligosaccharides in biofilm formation. Additionally, the LPS inner-core mutants ΔwaaF and ΔwaaC lost the ability to produce flagella and curli. Furthermore, these inner-core mutants exhibited a dramatic reduction in adherence to and invasion of epithelial cells (MAC-T), showing an approximately 100-fold decrease in cell invasion compared with the outer-core mutants (waaI and waaG) and the wild type. These findings underscore the critical role of LPS-core truncation in impairing flagella and curli biosynthesis, thereby reducing the invasion capability of E. coli O157 ATCC 43895.