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Nowadays, metal-organic frameworks (MOFs) have been emerged as an efficient platform for enzyme immobilization due to their high porosity, tunability, and chemical versatility. In this study, a series of hybrid lipase@NKMOF-101-M (M = Mg, Mn, Zn, Co, or Ni) biocatalysts were constructed through a facile in situ encapsulation method, and the encapsulation and immobilization of lipase in MOFs were carefully validated. The catalytic activity of lipase@NKMOF-101-Mn was 2-fold higher than that of lipase@ZIF-8 and 3-fold higher than that of lipase@MCM-41 due to its excellent dispersibility and hydrophobicity in hexane. The reduced K m value demonstrated a superior affinity of lipase@NKMOF-101s toward to the substrate in non-aqueous reaction system. Moreover, the effects of MOF particle size, metal ions, and enzyme distribution on the catalytic performance of the immobilized lipase were systematically investigated. The results demonstrated that as the particle size of lipase@NKMOF-101s decreased, the apparent enzyme activity increased dramatically. Metal ions in MOFs exhibited activation effect toward to enzyme activity and an approximate 12-fold increase in activity was achieved when transesterification was performed using lipase@NKMOF-101-Mn compared with free lipase. Notably, lipase@NKMOF-101-Co and lipase@NKMOF-101-Ni exhibited substrate selectivity owing to the specific distribution of the lipase in the MOF carriers. Lipase@NKMOF-101s can maintain >80 % of its initial activity even after 5 recycles and a long-term storage (30 days). Consequently, NKMOF-101 is a tunable and sustainable platform for the construction of enzyme@MOFs biocatalysts with superior catalytic performance., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025 Elsevier B.V. All rights reserved.)

Purpose: The color of Papanicolaou-stained specimens is a crucial feature in cytology diagnosis. However, the quantification of color using digital images is challenging due to the variations in the staining process and characteristics of imaging equipment. The dye amount estimation of stained specimens is helpful for quantitatively interpreting the color based on a physical model. It has been realized with color unmixing and applied to staining with three or fewer dyes. Nevertheless, the Papanicolaou stain comprises five dyes. Thus, we employ multispectral imaging with more channels for quantitative analysis of the Papanicolaou-stained cervical cytology samples., Approach: We estimate the dye amount map from a 14-band multispectral observation capturing a Papanicolaou-stained specimen using the actual measured spectral characteristics of the single-stained samples. The estimated dye amount maps were employed for the quantitative interpretation of the color of cytoplasmic mucin of lobular endocervical glandular hyperplasia (LEGH) and normal endocervical (EC) cells in a uterine cervical lesion., Results: We demonstrated the dye amount estimation performance of the proposed method using single-stain images and Papanicolaou-stain images. Moreover, the yellowish color in the LEGH cells is found to be interpreted with more orange G (OG) and less Eosin Y (EY) dye amounts. We also elucidated that LEGH and EC cells could be classified using linear classifiers from the dye amount., Conclusions: Multispectral imaging enables the quantitative analysis of dye amount maps of Papanicolaou-stained cytology specimens. The effectiveness is demonstrated in interpreting and classifying the cytoplasmic mucin of EC and LEGH cells in cervical cytology., (© 2024 The Authors.)