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As a continuation of our pharmacognostic studies on different Glycyrrhiza species growing in Türkiye, the phytochemical investigation of the roots of Glycyrrhiza asymmetrica Hub.-Mor., a licorice species endemic to Türkiye, was carried out. This study yielded twenty-three secondary metabolites ( 1 - 23 ) including nine previously unreported compounds: two dihydroaurone-3-enoic acids, licoaurone A ( 1 ) and licoaurone B ( 2 ), isoflavan hydroxypreglabridin ( 3 ), isoflavanone cyclodeoxykievitone ( 4 ), flavanone-3-ol glycyasymmetrol ( 5 ), and four bi-isoflavans, glycyasymmetrica A-D ( 6 - 9 ). The structures of isolated compounds were established by NMR and MS experiments. The relative configurations ( 6 - 9 ) were assigned by a combined quantum mechanical/NMR approach, comparing the experimental 13 C/ 1 H NMR chemical shift data and the related predicted values. The absolute configurations of compounds 1 - 9 were assigned by comparison of their experimental electronic circular dichroism curves with the TDDFT-predicted curves. All isolated compounds were also evaluated for their cytotoxic activity against MCF-7, HeLa, HepG2, and A549 cancer cell lines by using MTT assay.

Objective: The incidence of caesarean sections (CSs) has increased significantly in recent years, especially in developed countries. This study aimed to identify the factors that most influence the length of hospital stay (LOS) after a CS, using data from 9,900 women who underwent CS at the "Federico II" University Hospital of Naples between 2014 and 2021., Methods: Various artificial intelligence models were employed to analyze the relationships between the LOS and a set of independent variables, including maternal and foetal characteristics. The analysis focused on identifying the model with the best predictive performance and specific comorbidities impacting LOS., Results: A multiple linear regression model determined the highest R-value (0.815), indicating a strong correlation between the identified variables and LOS. Significant predictors of LOS included abnormal foetuses, cardiovascular disease, respiratory disorders, hypertension, haemorrhage, multiple births, preeclampsia, previous delivery complications, surgical complications, and preoperative LOS. In terms of classification models, the decision tree yielded the highest accuracy (75%)., Conclusions: The study concluded that certain comorbidities, such as cardiovascular disease and preeclampsia, significantly impact LOS following a CS. These findings can assist hospital management in optimizing resource allocation and reducing costs by focusing on the most influential factors., Competing Interests: Declarations. Ethics approval and consent to participate: In compliance with the Declaration of Helsinki and with the Italian Legislative Decree 211/2003, Implementation of the 2001/20/CE directive, since no patients/children were involved in the study, the signed informed consent form and ethical approval are not mandatory for these types of studies. Furthermore, in compliance with the regulations of the Italian National Institute of Health, our study is not reported among those needing assessment by the Ethical Committee of the Italian National Institute of Health. Consent for publication: Not applicable. Consent to participate: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)

The phytochemical study of Zanthoxylum rhoifolium apolar and medium polarity stem bark and leaf extracts afforded 29 compounds, including three new sesquiterpenes (1 - 3: ) and one new α -ionone glycoside (4: ). All compounds were characterized by means of 1D and 2D NMR and HRESIMS data. Furthermore, a precise structural analysis was performed, employing a combined density functional theory (DFT)/NMR approach to elucidate the compounds configurations. The crude extracts were then tested against a panel of gram-positive and gram-negative bacteria by broth dilution methods to determine their minimal inhibitory concentration. In these experimental conditions, no interesting antimicrobial activity was observed., Competing Interests: The authors declare that they have no conflict of interest., (Thieme. All rights reserved.)

Inhibiting microsomal prostaglandin E 2 synthase-1 (mPGES-1), an inducible enzyme involved in prostaglandin E 2 (PGE 2 ) biosynthesis and tumor microenvironment (TME) homeostasis, is a valuable strategy for treating inflammation and cancer. In this work, 5-methylcarboxamidepyrrole-based molecules were designed and synthesized as new compounds targeting mPGES-1. Remarkably, compounds 1f, 2b, 2c, and 2d were able to significantly reduce the activity of the isolated enzyme, showing IC 50 values in the low micromolar range. With the aim of further profiling the synthesized molecules, their ability to interfere with the activity of soluble epoxide hydrolase (sEH), whose inhibition blocks the loss of the anti-inflammatory mediators epoxyeicosatrienoic acids (EETs or epoxyicosatrienoic acids), was investigated in silico and by employing specific biological assays. Among the set of tested compounds, 1f, 2b, 2c, and 2d emerged as mPGES-1/sEH dual inhibitors. Moreover, given that overexpression of mPGES-1 has been observed in many human tumors, we finally explored the biological effect of our compounds in an in vitro model of human colorectal cancer (CRC). The obtained outcomes pave the way for future investigation to optimize and further characterize anticancer pharmacological profile of the carboxamidepyrrole-based molecules., (© 2024 The Author(s). Archiv der Pharmazie published by Wiley‐VCH GmbH on behalf of Deutsche Pharmazeutische Gesellschaft.)

Objective: To describe the simultaneous laparoscopic approach for teratoma removal and oocyte retrieval performed under regional anesthesia in a woman, desiring to preserve fertility. The patient included in this video gave consent for publication of the video and posting of the video online including social media, the journal website, scientific literature websites, and other applicable sites., Design: Video case report demonstrating the clinical management and laparoscopic teratoma removal combined with oocyte retrieval accomplished under regional anesthesia., Patient(s): We present a case of a 31-year-old woman who referred to our Fertility Center with a previous history of right salpingo-oophorectomy for mucinous ovarian cystadenoma and the presence of a large ovarian teratoma of ten centimeters of the contralateral ovary., Intervention(s): The ovarian stimulation started in the early follicular phase. Not being able to visualize follicular growth during the ovarian stimulation, it was decided to adopt fixed protocol with antagonist on the 5th day and to proceed, on 15th day, with a laparoscopic pick-up and simultaneous removal of the cyst., Main Outcome Measure(s): Laparoscopic surgery was performed: the left ovary appeared larger in size for the presence of the suspicious mature dermoid cyst and multiple follicles, previously not identified at ultrasound imaging. The follicles contained oocytes that were aspirated with an aspiration needle of 17 G connected to a craft suction pump. Then, practicing the stripping technique, an enucleation of the ovarian cyst was performed. Surprisingly, the removal of the cyst revealed other follicles that were readily aspirated., Result(s): The patient remained awake during the entire procedure and a low pressure of 10 mmHg was maintained at 15° of Trendelenburg position. A total of seven follicles were aspirated, seven oocytes were retrieved, and six mature oocytes were cryopreserved. The cyst was totally removed and no intracavitary spillage was caused., Conclusion(s): Simultaneous laparoscopic approach for teratoma removal and oocyte cryopreservation should be considered an effective fertility preservation strategy in patients in whom the presence of an ovarian neoformation does not allow visualization of growing follicles by ultrasound. Laparoscopic oocyte retrieval under regional anesthesia is a safe and well-tolerated technique and should be considered for patients where the transvaginal approach could not be performed., Competing Interests: Declaration of Interests C.A. has nothing to disclose. G.G.I. has nothing to disclose. P.S. has nothing to disclose. M.D.A. has nothing to disclose. G.B. has nothing to disclose. P.G. has nothing to disclose., (Copyright © 2024 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.)