1. Fucosylated Chondroitin Sulfate from Bohadschia ocellata : Structure Analysis and Bioactivities.
- Author
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Thinh, Pham Duc, Cao, Hang Thi Thuy, Trung, Dinh Thanh, Minh, Duong Khanh, Cao, Thao Quyen, Van, Tran Thi Thanh, Zueva, Anastasia O., Ermakova, Svetlana P., and Nguyen, Thanh-Danh
- Subjects
PARTIAL thromboplastin time ,PROTEIN-tyrosine phosphatase ,PHOSPHOPROTEIN phosphatases ,ANALYTICAL chemistry ,TYPE 2 diabetes - Abstract
Fucosylated chondroitin sulfate (FCS) was prepared from Bohadschia ocellata using protease hydrolysis. The structural characteristics of FCS were confirmed through chemical composition analysis using FTIR spectroscopy,
1 H NMR, and13 C NMR. FCS from B. ocellata (FCS-Bo) exhibited an average molecular weight of approximately 122 kDa. The biological activities of FCS-Bo, including anticoagulant, anti-cancer, and Protein Tyrosine Phosphatase 1B (PTP1B) inhibition, were evaluated. FCS-Bo displayed potent anticoagulant properties, markedly extending activated partial thromboplastin time, prothrombin time, and thrombin time when compared to the heparin control. In anti-cancer bioactivity research, FCS-Bo efficiently inhibited colony formation in the colon cancer cell lines HCT-116, HT-29, and DLD-1, achieving inhibition rates of up to 65%. Additionally, FCS-Bo exhibited significant inhibition of PTP1B, with an IC50 as low as 0.0326 µg/mL, suggesting its potential for improving insulin sensitivity and managing conditions such as type 2 diabetes and obesity. [ABSTRACT FROM AUTHOR]- Published
- 2024
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