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• Conventional visual fatigue assessments cannot detect dynamic fatigue changes. • Dynamic visual fatigue assessment is proposed to measure fatigue changes. • Results are validated using SSQ analysis and video content features. • Video content features predict visual fatigue changes efficiently. • Dynamic visual fatigue significantly correlates with objective video features. People watching video displays for long durations experience visual fatigue and other symptoms associated with visual discomfort. Fatigue-reduction techniques are often applied but may potentially degrade the immersive experience. To appropriately adjust fatigue-reduction techniques, the changes in visual fatigue over time should be analyzed which is crucial for the appropriate adjustment of fatigue-reduction techniques. However, conventional methods used for assessing visual fatigue are inadequate because they rely entirely on post-task surveys, which cannot easily determine dynamic changes. This study employed a dynamic assessment method for evaluating visual fatigue in real-time. Using a joystick, participants continuously evaluated subjective fatigue whenever they perceived changes. A Simulator Sickness Questionnaire (SSQ) validated the results, which indicated significant correlations between dynamic assessments and the SSQ across five items associated with symptoms associated with visual discomfort. Furthermore, we explored the potential relationship between dynamic visual fatigue and objective video features, e.g., optical flow and the V-values of the hue/saturation value (HSV) color space, which represent the motion and brightness of the video. The results revealed that dynamic visual fatigue significantly correlated with both the optical flow and the V-value. Moreover, based on machine learning models, we determined that the changes in visual fatigue can be predicted based on the optical flow and V-value. Overall, the results validate that dynamic assessment methods can form a reliable baseline for real-time prediction of visual fatigue. [ABSTRACT FROM AUTHOR]