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It is relevant to study of angiogenesis mediators and the mobilization of early endothelial progenitor cells (EPС) from bone marrow into the blood in patients with coronary heart disease (CHD), suffering and not suffering from ischemic cardiomyopathy (ICMP).СHD patients: 30 people with ICMP and 22 people without ICMP, 15 healthy donors. The content of early EPС (VEGFR2+CD34+CD14+) was determined in the blood and bone marrow by flow cytofluorometry, the concentration of MSP-1, SDF-1, VEGF-A – by multiplex analysis, of HIF-1α – by ELISA.Тhe content of SDF-1 and HIF-1α in peripheral blood in patients with CHD without cardiomyopathy was higher than in healthy individuals (respectively 60.00 (50.00-80.00) pg/mL and 6.00 (5.00-6.20) ng/mL versus 30.00 (5.00-45.00) pg/mL, p = 0.049 and 4.60 (3.28-5.11) ng/mL, p = 0.049), with ICMP corresponded to the norm. Тhe concentration of SDF-1 in the bone marrow was higher, and the level of HIF-1α was less than their content in the bloodstream, regardless of the presence of ICMP (respectively 130.0 (90.0-170.0) pg/mL, p = 0.005 and 0.97 (0.80-1.11) ng/mL, p < 0.001). The level of MCP-1 in the blood varied within the normal range in patients with CHD of both study groups (190.0 (168.0-215.0) pg/mL), and in the bone marrow was higher only in patients with ICMP (406.5 (265.0-583.0) pg/mL, p = 0.028). Regardless of ICMP presence, the content of VEGF-A in the blood of patients with CHD corresponded to the norm (3.80 (1.00-6.50) pg/mL) and in myeloid tissue. The number of EPC was increased in the blood of patients with CHD without cardiomyopathy (0.70 (0.46-1.23) and 0.19 (0.13-0.32) %, p < 0.001) and corresponded to their number in the bone marrow. And in patients with ICMP, normal values of the indicator were recorded in the blood with the accumulation of EPC in myeloid tissue (0.57 (0.45-0.98) %, p = 0.019).The development of ICMP is associated with the accumulation of early EPC in myeloid tissue due to their increased retention by an excess of MCP-1 in the bone marrow with weak involvement in the bloodstream due to the lack of a surplus of SDF-1 and HIF-1a in the blood.

Aim. To discuss the functional properties of melatonin which plays a key role in the expression of psychopathological disorders following traumatic brain injury (TBI). The influence of melatonin on neurochemical changes, which may lead to various mental disorders, has been analyzed. Specifically, its impact on asthenia, cognitive impairments, emotional instability, and insomnia, which are among the main symptoms accompanying TBI in the chronic phase, has been examined. The importance of studies on melatonin as a neurotransmitter and its role in the circadian rhythm regulation, which is often impaired as a consequence of TBI, has been emphasized. The mechanisms underlying these disorders may be related to dysfunction of the hypothalamus and interhemispheric interactions. Melatonin may influence the recovery processes after TBI, reducing inflammation and providing optimal conditions for tissue regeneration. It opens up new possibilities for the development of therapy aimed at reducing the impact of trauma consequences on patients. Earlier studies have also indicated the option of using melatonin as an antioxidant, which can protect brain tissue from further damage caused by recovery processes after injury. Studies on pharmacological modulation of the melatonin system could be useful for developing new methods for treatment and prevention of mental disorders associated with TBI. Examining the action of melatonin receptor antagonists and agonists can help to choose optimal treatment strategies aimed at correcting disorders arising as a result of trauma. It holds promise for faster and more complete recovery of mental health in TBI patients. Conclusions. Considering the significance of melatonin in regulation of physiological processes, further studies may enrich understanding of its role, ensuring the development of more effective treatment methods. Given the safe profile of melatonin and its availability as a supplement, supportive studies in this direction have the potential to positively impact clinical practice.

Background. In the pathogenesis of ischemic cardiomyopathy (ICMP), angiopoiesis remains unexplored.The aim. To describe the vasculature of the heart and the imbalance of angiogenesis mediators in the coronary circulation in association with the number of endothelial progenitor cells (EPC) and desquamated endothelial cells (DEC) in the blood of patients with coronary heart disease (CHD), suffering and not suffering from ICMP.Methods. Fifty-two patients with CHD (30 patients with ICMP, 22 patients without ICMP), 15 healthy donors were examined. The content of EPC (CD14+CD34+VEGFR2+) in the blood from the cubital vein and DEC (CD45–CD146+) in the blood from the coronary sinus and the cubital vein was determined by flow cytometry. The concentrations of VEGF-A (vascular endothelial growth factor A), PDGF (platelet-derived growth factor), and SDF-1 (stromal cell-derived factor 1) in blood plasma were recorded using immunofluorescence assay; the angiopoietin-2, MMP-9 (matrix metallopeptidase 9) were recorded using enzyme immunoassay. In myocardial biopsies the specific area of vessels and the expression of αSMA (smooth muscle alpha-actin) were determined by morphometric and immunohistochemical methods.Results. In the peripheral blood of patients with CHD, regardless of the presence of ICMP, the DEC content exceeded the physiological level, and the VEGF-A, PDGF, angiopoietin-2, and MMP-9 corresponded to the norm. In CHD patients without cardiomyopathy, there was an excess of SDF-1 and EPC in the blood from the cubital vein, and in ICMP, their physiological significance was noted. In the coronary blood flow in patients with CHD without cardiomyopathy, an increase in the concentration of PDGF was found, which was not determined in patients with ICMP, who had an increased content of DEC, angiopoietin-2 and MMP-9. The specific area of the vessels in the patients of the two groups was comparable; the expression of αSMA in ICMP was 6.2 times lower than in patients with CHD without cardiomyopathy.Conclusion. The development of ICMP is accompanied by impaired maturation of vessels in the myocardium, associated with the absence of a compensatory reaction of activation of cellular and humoral factors of angiogenesis.

The combination effect of substitutional impurities of group IVB–VIB transition metals on the oxygen vacancy formation energy in rutile titania was studied by the projector augmented wave method within density functional theory. The pair interaction energy of impurity atoms was estimated depending on the interatomic distance. It was shown that the interaction in the Zr + Zr, Hf + Hf and Nb + Ta pairs leads to the energy preference of their orientation along the <100> direction at a distance of the lattice parameter a. The Mo + Mo and Nb + Mo pairs prefer to orient along the [001] direction at a distance of the lattice parameter c. If the impurity atoms are farther than the third neighbors, then their interaction can be neglected. It was found that the change in the oxygen vacancy formation energy due to doping with several impurities can be estimated as the sum of the energy changes due to single impurity divided by a coefficient whose value depends on the mutual arrangement of the impurity atoms. [ABSTRACT FROM AUTHOR]

Circular RNAs (circRNAs) are a large class of endogenous single-stranded covalently closed RNA molecules. High-throughput RNA sequencing and bioinformatic algorithms have identified thousands of eukaryotic circRNAs characterized by high stability and tissue-specific expression pattern. Recent studies have shown that circRNAs play an important role in the regulation of physiological processes in the norm and in various diseases, including cardiovascular disorders. The review presents current concepts of circRNA biogenesis, structural features, and biological functions, describes the methods of circRNA analysis, and summarizes the results of studies on the role of circRNAs in the pathogenesis of hypertrophic cardiomyopathy, the most common inherited heart disease. [ABSTRACT FROM AUTHOR]