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AIM: To evaluate the efficacy, timing of retreatment and safety of dexamethasone (DEX) implant on macular edema (ME) secondary to diabetic retinopathy (DME) and retinal vein occlusion (RVO-ME) patients who were refractory to anti-vascular endothelial growth factor (VEGF) treatment. METHODS: This retrospective study included 37 eyes received at least one DEX implant treatment for DME or RVO-ME between January 1, 2019, and January 1, 2023. These refractory DME and RVO-ME cases received at least 5 anti-VEGF injections and failure to gain more than 5 letters or a significant reduction in central retinal thickness (CRT). The best corrected visual acuity (BCVA) and CRT were measured at baseline, and at 1, 3, 4 and 6mo post-DEX implant injection. Adverse events such as elevated intraocular pressure (IOP) and cataract were recorded. RESULTS: For RVO cases (n=22), there was a significant increase in BCVA from 0.27±0.19 to 0.35±0.20 at 6mo post-DEX injection (P

Abstract Lettuce is one of the most widely cultivated and consumed dicotyledonous vegetables globally. Despite the availability of its reference genome sequence, lettuce gene annotation remains incomplete, impeding comprehensive research and the broad application of genomic resources. Long-read RNA isoform sequencing (Iso-Seq) offers substantial advantages for analyzing RNA alternative splicing and aiding gene annotation, yet it faces throughput limitations. We present the HIT-ISOseq method tailored for bulk sample analysis, significantly enhancing RNA sequencing throughput on the PacBio platform by concatenating cDNA. Here we show, HIT-ISOseq generates 3-4 cDNA molecules per CCS read in lettuce, yielding 15.7 million long reads per PacBio Sequel II SMRT Cell 8 M. We validate its effectiveness in analyzing six lettuce tissue samples, including roots, stems, and leaves, revealing tissue-specific gene expression patterns and RNA isoforms. Leveraging diverse tissue long-read RNA sequencing, we refine the transcript annotation of the lettuce reference genome, expanding its GO and KEGG annotation repertoire. Collectively, this study serves as a foundational reference for genome annotation and the analysis of multi-sample isoform expression, utilizing high-throughput long-read transcriptome sequencing.

To enhance the transmission capabilities in power system scheduling, this paper develops a unit commitment model that incorporates dynamic transmission line capacities and proposes an efficient solving algorithm. A multi-scenario unit commitment model that integrates dynamic transmission line capacities is introduced, using quantile regression to construct a data-driven capacity increase model based on historical environmental data. The model is solved using Lagrangian relaxation and the Alternating Direction Method of Multipliers (ADMM) to decouple dynamic constraints, allowing the dual problem to be decomposed into sub-problems and solved iteratively. The proposed model and algorithm are validated using the IEEE-118 and IEEE-300 test cases, demonstrating their effectiveness in handling dynamic transmission line capacities and improving scheduling performance.The approach provides a robust and flexible solution for power system scheduling, enhancing reliability and economic efficiency.

Abstract Voice identification is being increasingly adopted in various domains, including security infrastructures, intelligent home systems, and personalised digital assistants. Notably, it harbours significant promise in transforming healthcare, especially in electronic health record detecting and speech impairment monitoring such as aphasia. Current strategies such as acoustic models based on deep learning, voice bio‐metrics, and spectrogram analysis, have been identified with several drawbacks including vulnerability to altered voices, susceptibility to ambient noise, and the necessity for significant computational power. In response to these issues, the authors introduce a ground‐breaking method of voice identification using Ultra‐Wideband (UWB) technology. This method capitalises on the micro‐Doppler shifts associated with movements of the laryngeal prominence. The distinctive nature of these bio‐metric traits related to speech production provides superior resistance against common pitfalls of voice identification. The proposed model leverages the high‐resolution characteristics of UWB to register tiny variations in laryngeal movements produced during speech, thus forming a distinct voice profile for each speaker. Through rigorous testing, the proposed system demonstrated significant progress in voice identification, achieving close to 90% accuracy in controlled experimental settings. This breakthrough indicates that UWB‐enabled voice identification could have a profound effect on medical applications, providing potential improvements in diagnosing, monitoring, possibly treating speech disorders, and thereby shaping a future of enhanced and secured healthcare services.

The sliding mode observer (SMO) has the advantages of small influence by parameter changes and strong robustness, which is widely used in the sensorless control of permanent magnet synchronous motor (PMSM). However, when the sliding mode observer is used to observe the position and speed information of the PMSM, the problem of slow response and excessive chattering is always accompanied. In order to reduce the time of observation position and improve the anti-interference of the system, the super-twisting sliding mode observer(STSMO) is proposed, which can be used to reduce the chattering amplitude effectively, Additionally, the fast and terminal factors is added to the sliding mode surface so that it can converge quickly in a finite time. Then, an improved fast terminal super-twisting sliding mode observer (FTSTSMO) is proposed in the extended state. It can be proved that the observer can converge by Lyapunov stability, and the new observer can suppress chattering and improve the convergence speed. Finally, the experimental analysis is carried out on the 1kW permanent magnet synchronous motor experimental platform, and the SMO and STSMO are compared with FTSTSMO. The results show that FTSTSMO can effectively reduce the fluctuation of the system, improve the tracking effect of rotor speed and rotor position when the speed changes, and further make the whole control system of PMSM have stronger robustness and stability.

As the genome is organized into a three-dimensional structure in intracellular space, epigenomic information also has a complex spatial arrangement. However, most epigenetic studies describe locations of methylation marks, chromatin accessibility regions, and histone modifications in the horizontal dimension. Proper spatial epigenomic information has rarely been obtained. In this study, we designed spatial chromatin accessibility sequencing (SCA-seq) to resolve the genome conformation by capturing the epigenetic information in single-molecular resolution while simultaneously resolving the genome conformation. Using SCA-seq, we are able to examine the spatial interaction of chromatin accessibility (e.g. enhancer–promoter contacts), CpG island methylation, and spatial insulating functions of the CCCTC-binding factor. We demonstrate that SCA-seq paves the way to explore the mechanism of epigenetic interactions and extends our knowledge in 3D packaging of DNA in the nucleus.

Wearing masks is an effective protective measure for residents to prevent respiratory infectious diseases when going out. Due to issues such as a small target size, target occlusion leading to information loss, false positives, and missed detections, the effectiveness of face mask-wearing detection needs improvement. To address these issues, an improved YOLOv7 object detection model is proposed. Firstly, the C2f_SCConv module is introduced in the backbone network to replace some ELAN modules for feature extraction, enhancing the detection performance of small targets. Next, the SPPFCSPCA module is proposed to optimize the spatial pyramid pooling structure, accelerating the model convergence speed and improving detection accuracy. Finally, the HAM_Detect decoupled detection head structure is introduced to mitigate missed and false detections caused by target occlusion, further accelerating model convergence and improving detection performance in complex environments. The experimental results show that improved YOLOv7 achieved an mAP of 90.1% on the test set, a 1.4% improvement over the original YOLOv7 model. The detection accuracy of each category improved, effectively providing technical support for mask-wearing detection in complex environments.

Semantic segmentation of human images is a research hotspot in the field of computer vision. At present, the semantic segmentation models based on U-net generally lack the ability to capture the spatial information of images. At the same time, semantic incompatibility exists because the feature maps of encoder and decoder are directly connected in the skip connection stage. In addition, in low light scenes such as at night, it is easy for false segmentation and segmentation accuracy to appear. To solve the above problems, a portrait semantic segmentation method based on dual-modal information complementarity is proposed. The encoder adopts a double branch structure, and uses a SK-ASSP module that can adaptively adjust the convolution weights of different receptor fields to extract features in RGB and gray image modes respectively, and carries out cross-modal information complementarity and feature fusion. A hybrid attention mechanism is used in the jump connection phase to capture both the channel and coordinate context information of the image. Experiments on human matting dataset show that the PA and MIoU coefficients of this algorithm model reach 96.58% and 94.48% respectively, which is better than U-net benchmark model and other mainstream semantic segmentation models.