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Abstract To accurately understand the deformation behavior of surrounding rock in the jiugongshan No.2 high ground stress tunnel and optimize construction parameters for improved efficiency, on-site monitoring was used to gather data on rock deformation and initial support contact pressure. A WOA-LSTM regression prediction model was proposed, and excavation advance depth was optimized using numerical simulation. The WOA-LSTM regression prediction model demonstrated high accuracy in tunnel deformation monitoring. The absolute errors between predicted and actual values of tunnel settlement and convergence at various measurement points average 2.53− 04 mm and 1.96− 04 mm, with relative errors averaging 2.15% and 2.34%. These results meet the requirements for guiding construction. Additionally, based on the results of numerical simulation calculations, when the step length is 12 m during the construction of high-stress tunnels using the benching method, excavation advances of 2 m and 3 m result in settlement and convergence increases of 35.1% and 63.4%, and 25.5% and 55.2%, respectively, compared to an excavation advance of 1 m. However, no sudden jumps in these values were observed, indicating that with a 3 m excavation advance, the integrity of the surrounding rock remains in good condition, effectively guiding safe and rapid construction methods.

Abstract Background Inflammatory bowel disease (IBD), a chronic inflammatory condition, is caused by several factors involving aberrant immune responses. Genetic factors are crucial in IBD occurrence. Mendelian randomization (MR) can offer a new perspective in understanding IBD’s genetic background. Methods Single nucleotide polymorphisms (SNPs) were considered instrumental variables (IVs). We analyzed the relationship between 731 immunophenotypes, 1,400 metabolite phenotypes, and IBD. The total effect was decomposed into indirect and direct effects, and the ratio of the indirect effect to the total effect was calculated. Results We identified the causal effects of HLA-DR-expressing CD14 + monocytes on IBD through MR analysis. The phenotype “HLA-DR expression on CD14 + monocytes” showed the strongest association among the selected 48 immune phenotypes. Chiro-inositol metabolites mediated the effect of CD14 + monocytes expressing HLA-DR on IBD. An increase in Chiro-inositol metabolites was associated with a reduced risk of IBD occurrence, accounting for 4.97%. Conclusion Our findings revealed a new pathway by which HLA-DR-expressing CD14 + monocytes indirectly reduced the risk of IBD occurrence by increasing the levels of Chiro-inositol metabolites. The results provided a new perspective on the immunoregulatory mechanisms underlying IBD, laying a theoretical foundation for developing new therapeutic targets in the future.

BackgroundTertiary hospitals in China are confronted with significant challenges due to limited spatial capacity and workforce constraints, leading to saturated allocation of medical resources and restricted growth in medical service provision. The incorporation of digital health into medical service process reengineering (MSPR) marks a pivotal transformation and restructuring of conventional health service delivery models. Specifically, the application of MSPR to blood sampling services processes reengineering (BSSPR) holds promise for substantially enhancing the efficiency and quality of medical services through streamlining and optimizing these procedures. However, the comprehensive impact of BSSPR has been infrequently quantified in existing research. ObjectiveThis study aims to investigate the influence of BSSPR on the efficiency and quality of medical services and to elucidate the key informative technological support points underpinning BSSPR. MethodsData were collected from both the new and old laboratory information systems from August 1, 2019, to December 31, 2021. A combination of statistical description, chi-square test, and t test was used to compare check-in time and waiting time of outpatients before and after the implementation of BSSPR. An interrupted time-series design was used to analyze the impact of BSSPR on medical service efficiency and quality, enabling the control of confounding variables, including changes in medical human resources and both long- and short-term temporal trends. ResultsBSSPR had an impact on the efficiency and quality of medical services. Notably, there was a significant increase in the number of patients receiving blood sampling services, with a daily service volume increase of ~150 individuals (P=.04). The average waiting time for patients decreased substantially from 29 (SD 36) to 11 (SD 11) minutes, indicating a marked improvement in patient experience. During the peak period, the number of patients receiving blood sampling services per working hour statistically increased from 9.56 to 16.77 (P

Background: To enhance the accuracy of hepatocellular carcinoma (HCC) diagnosis using contrast-enhanced (CE) US, the American College of Radiology developed the CEUS Liver Imaging Reporting and Data System (LI-RADS). However, the system still exhibits limitations in distinguishing between HCC and non-HCC lesions. Purpose: To investigate the viability of employing machine learning methods based on quantitative parameters of contrast-enhanced ultrasound for distinguishing HCC within LR-M nodules. Materials and methods: This retrospective analysis was conducted on pre-treatment CEUS data from liver nodule patients across multiple centers between January 2013 and June 2022. Quantitative analysis was performed using CEUS images, and the machine learning diagnostic models based on quantitative parameters were utilized for the classification diagnosis of LR-M nodules. The performance of the model was assessed using the area under the receiver operating characteristic curve (AUC) and compared with the performance of four radiologists. Results: The training and internal testing datasets comprised 168 patients (median age, 53 years [IQR, 18 years]), while the external testing datasets from two other centers included 110 patients (median age, 54 years [IQR, 16 years]). In the internal independent test set, the top-performing Random Forest model achieved an AUC of 0.796 (95%CI: 0.729–0.853) for diagnosing HCC. This model exhibited a sensitivity of 0.752 (95%CI: 0.750–0.755) and a specificity of 0.761 (95%CI: 0.758–0.764), outperforming junior radiologists who achieved an AUC of 0.619 (95%CI: 0.543–0.691, p

Objectives: Patients with intermediate or advanced hepatocellular carcinoma (HCC) require repeated disease monitoring, prognosis assessment and treatment planning. In 2018, a novel machine learning methodology “survival path” (SP) was developed to facilitate dynamic prognosis prediction and treatment planning. One year after, a deep learning approach called Dynamic Deephit was developed. The performance of the two state-of-art models in dynamic prognostication have not been compared. Methods: We trained and tested the SP and Dynamic DeepHit models in a large cohort of 2511 HCC patients using time-series data. The time-series data were converted into data of time slices, with an interval of three months. The time-dependent c-index for OS at given prediction time ( t = 1, 6, 12, 18 months) and evaluation time (∆ t = 3, 6, 9, 12, 18, 24, 36, 48 months) were compared. Results: The comparison between SP model and Dynamic DeepHit-HCC model showed the latter had significant better performance at the time of initial admission. The time-dependent c-index of Dynamic DeepHit-HCC model gradually decreased with the extension of time (from 0.756 to 0.639 in the training set; from 0.787 to 0.661 in internal testing set; from 0.725 to 0.668 in multicenter testing set); while the time-dependent c-index of SP model displayed an increased trend (from 0.665 to 0.748 in the training set; from 0.608 to 0.743 in internal testing set; from 0.643 to 0.720 in multicenter testing set). When the prediction time comes to 6 months or later since initial treatment, the survival path model outperformed the dynamic DeepHit model at late evaluation times (∆ t > 12 months). Conclusions: This research highlighted the unique strengths of both models. The SP model had advantage in long term prediction while the Dynamic DeepHit-HCC model had advantages in prediction at near time points. Fine selection of models is needed in dealing with different scenarios.

Abstract Although most people living with HIV (PLWH) receiving antiretroviral therapy (ART) achieve continuous viral suppression, some show detectable HIV RNA as low-level viremia (LLV) (50–999 copies/mL). Drug resistance mutations (DRMs) in PLWH with LLV is of particular concern as which may lead to treatment failure. In this study, we investigated the prevalence of LLV and LLV-associated DRMs in PLWH in Zhengzhou City, China. Of 3616 ART-experienced PLWH in a long-term follow-up cohort from Jan 2022 to Aug 2023, 120 were identified as having LLV. Of these PLWH with LLV, we obtained partial pol and integrase sequences from 104 (70 from HIV-1 RNA and 34 from proviral DNA) individuals. DRMs were identified in 44 individuals. Subtyping analysis indicated that the top three subtypes were B (48.08%, 50/104), CRF07_BC (31.73%, 33/104), and CRF01_AE (15.38%, 16/104). The proportions of nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs), protease inhibitors (PIs), and integrase strand transfer inhibitors (INSTIs) associated DRMs were 23.83% (24/104), 35.58% (37/104), 5.77% (6/104), and 3.85% (4/104), respectively, which contributed to an overall prevalence of 42.31% (44/104). When analyzed by individual DRMs, the most common mutation(s) were V184 (18.27%, 19/104), followed by V179 (11.54%, 12/104), K103 (9.62%, 10/104), Y181 (9.62%, 10/104), M41 (7.69%, 8/104), and K65R (7.69%, 8/104). The prevalence of DRMs in ART-experienced PLWH with LLV is high in Zhengzhou City and continuous surveillance can facilitate early intervention and provision of effective treatment.

Shaanxi province is rich in coal resources and is one of the main coal-producing provinces in China, with a coal production of 746 million tons in 2022. Due to the obvious differences in geological and hydrogeological conditions in the coal mining areas of the province, there are various types of water disasters in its coal mines. With the increase of coal mining intensity in recent years, the frequency of water disasters increases significantly, and the situation of water prevention and control is challenging. On the basis of the overall analysis of the hydrogeological conditions in the coal mining areas of Shaanxi province, combined with the types of water disasters revealed in the mining process of various mining areas in the province, the regional distribution of all kinds of water disas-ters is systematically summarized, and the formation mechanism and characteristics of typical water disasters are discussed. Also, the corresponding prevention and control technology and progress are put forward. The results show that ① the main coal producing areas in Shaanxi province are divided into northern Shaanxi, Huanglong and Weibei. The Jurassic coalfield in northern Shaanxi is mainly affected by the water disaster of roof's loose sand layer, the water disaster of thick sandstone, water and sand burst disaster, and the burnt rock flood exists in some coal mines. The Huanglong coalfield is mainly threatened by roof super-thick sandstone water, separated layer water, mud and sand burst disaster or hidden danger. The Carboniferous-Permian coalfields in northern Shaanxi and Weibei are mainly threatened by the water damage of Ordovician limestone on the coal seam floor. ② The hidden danger of roof flood in the Jurassic coalfield in northern Shaanxi is mainly formed by the disturbance of roof aquifer by mining water diversion fracture zone, which can form a continuous large flow water gushing through sandstone aquifer or loose sand layer, and the water filling intensity is large in some areas. Roof flood occurs when the amount of water exceeds the capacity of the drainage system in a short time, and it can be transformed into water and sand burst disaster when the collapse zone in the thin bedrock area directly leads to the loose sand layer. When the water-conducting fracture zone affects the water-rich area of burnt rock, it can form a flood of burnt rock with large instantaneous water volume and continuous flood. The super-thick sandstone aquifer on the roof of the Huanglong coalfield is swept by the mining water-conducting fracture zone to form a high-intensity continuous water gushing and may form roof flood. When the thickness of the lower aquifer is large, the separated layer water burst disaster may be formed. When the roof strata near the coal seam is soft and easy to collapse, it can be transformed into mud and sand burst disaster. In the Carboniferous-Permian coalfield and the Weibei coalfield in northern Shaanxi, when structures such as coal seam mining floor disturbance failure zones or faults lead to the Ordovician limestone aquifers, they will cause extremely serious floor water inrush disasters, which are characterized by strong sudden occurrence and large instantaneous water volume. ③ The roof water disaster treatment technology in each region mainly includes drilling hole drainage, grouting treatment, mining parameter control and so on, and the floor water damage is mainly treated by regional grouting reinforcement and plugging. Burnt rock water disaster is mainly treated by the combination of curtain grouting and drilling exploration.

Isoflavonoids, the major secondary metabolites within the flavonoid biosynthetic pathway, play important roles in plant defense and exhibit free radical scavenging properties in mammals. Recent advancements in understanding the synthesis, transport, and regulation of isoflavonoids have identified their biosynthetic pathways as promising targets for metabolic engineering, offering potential benefits such as enhanced plant resistance, improved biomass, and restoration of soil fertility. This review provides an overview of recent breakthroughs in isoflavonoid biosynthesis, encompassing key enzymes in the biosynthetic pathway, transporters influencing their subcellular localization, molecular mechanisms regulating the metabolic pathway (including transcriptional and post-transcriptional regulation, as well as epigenetic modifications). Metabolic engineering strategies aimed at boosting isoflavonoid content in both leguminous and non-leguminous plants. Additionally, we discuss emerging technologies and resources for precise isoflavonoid regulation. This comprehensive review primarily focuses on model plants and crops, offering insights for more effective and sustainable metabolic engineering approaches to enhance nutritional quality and stress tolerance.

Summary: Accurate tumor diagnosis by pathologists relies on identifying specific morphological characteristics. However, summarizing these unique morphological features in tumor classifications can be challenging. Although deep learning models have been extensively studied for tumor classification, their indirect and subjective interpretation obstructs pathologists from comprehending the model and discerning the morphological features accountable for classifications. In this study, we introduce a new approach utilizing Style Generative Adversarial Networks, which enables a direct interpretation of deep learning models to detect significant morphological characteristics within datasets representing patients with deficient mismatch repair/microsatellite instability-high gastric cancer. Our approach effectively identifies distinct morphological features crucial for tumor classification, offering valuable insights for pathologists to enhance diagnostic accuracy and foster professional growth.

As the main contributor of carbon emissions, the low-carbon transition of the industrial sector is important for achieving the goal of carbon dioxide peaking. Hydrogen-enabled industrial energy systems (HIESs) are a promising way to achieve the low-carbon transition of industrial energy systems, since the hydrogen can be well coordinated with renewable energy sources and satisfy the high and continuous industrial energy demand. In this paper, the long-term capacity expansion planning problem of the HIES is formulated from the perspective of industrial parks, and the targets of carbon dioxide peaking and the gradual decommissioning of existing equipment are considered as constraints. The results show that the targets of carbon dioxide peaking before different years or with different emission reduction targets can be achieved through the developed method, while the economic performance is ensured to some extent. Meanwhile, the overall cost of the strategy based on purchasing emission allowance is three times more than the cost of the strategy obtained by the developed method, while the emissions of the two strategies are same. In addition, long-term carbon reduction policies and optimistic expectations for new energy technologies will help industrial parks build more new energy equipment for clean transformation.

The linear and nonlinear dynamics of a bolted cylindrical shell under a point excitation were experimentally investigated. The tests have been carried out through methods of the hammer test and sweeping frequency test. This paper aims to discover the effect of bolt joints on shell vibration, including the effects of bolt number, tightening torque, and excitation levels. The natural characteristics were analyzed by utilizing natural frequency and mode shapes. The nonlinear dynamic characteristics were analyzed through amplitude-frequency curves. The resonance frequency, resonance amplitude, and damping ratio under different tightening torques and excitation levels were investigated. Results show that: the number of bolts can not only change the value and order of the natural frequencies but also the shapes of the mode; The low tightening torque and larger excitation levels strongly lead to more complex nonlinear dynamics of shell structures.

Isoflavonoids, the major secondary metabolites within the flavonoid biosynthetic pathway, play important roles in plant defense and exhibit free radical scavenging properties in mammals. Recent advancements in understanding the synthesis, transport, and regulation of isoflavonoids have identified their biosynthetic pathways as promising targets for metabolic engineering, offering potential benefits such as enhanced plant resistance, improved biomass, and restoration of soil fertility. This review provides an overview of recent breakthroughs in isoflavonoid biosynthesis, encompassing key enzymes in the biosynthetic pathway, transporters influencing their subcellular localization, molecular mechanisms regulating the metabolic pathway (including transcriptional and post-transcriptional regulation, as well as epigenetic modifications). Metabolic engineering strategies aimed at boosting isoflavonoid content in both leguminous and non-leguminous plants. Additionally, we discuss emerging technologies and resources for precise isoflavonoid regulation. This comprehensive review primarily focuses on model plants and crops, offering insights for more effective and sustainable metabolic engineering approaches to enhance nutritional quality and stress tolerance. [ABSTRACT FROM AUTHOR]

Abstract Modulating and elevating the operating voltage of a given cathode is a significant challenge to enhance the energy density of secondary batteries without sacrificing power output. The chemical coordination strongly influences the energy levels of d-orbitals of redox cations in cathode materials, which tie to their operating voltage. In contrast to concentrated studies on enhancing the specific capacity, in this study, we choose bi-layered hydrated vanadium pentoxide as the model to modulate the d-orbital energy levels through local chemical coordination manipulation, achieving a higher operating voltage in rechargeable aqueous zinc ion batteries. Here we show that, by employing X-ray absorption spectroscopy (XAS) and pair distribution function (PDF) techniques, we can analyze the distortion of [VO6] octahedra and extract chemical bond information, deciphering the correlation between the chemical coordination and operating voltage in cathode materials. The fundamentals could guide the designing and developing RAZIBs with higher energy and power density.

Abstract T helper cells, particularly T follicular helper (TFH) cells, are essential for the neutralizing antibody production elicited by pathogens or vaccines. However, in immunocompromised individuals, the inefficient support from TFH cells could lead to limited protection after vaccine inoculation. Here we showed that the conjugation of inducible T cell costimulatory (ICOS) onto the nanoparticle, together with immunogen, significantly enhanced the immune response of the vaccines specific for SARS-CoV-2 or human immunodeficiency virus type-1 (HIV-1) in TFH-deficient mice. Further studies indicated that ICOSL on B cells was triggered by ICOS binding, subsequently activated the PKCβ signaling pathway, and enhanced the survival and proliferation of B cells. Our findings revealed that the stimulation of ICOS-ICOSL interaction by adding ICOS on the nanoparticle vaccine significantly substitutes the function of TFH cells to support B cell response, which is significant for the immunocompromised people, such as the elderly or HIV-1-infected individuals.

Articular cartilage defects often involve damage to both the cartilage and subchondral bone, requiring a scaffold that can meet the unique needs of each tissue type and establish an effective barrier between the bone and cartilage. In this study, we used 3D printing technology to fabricate a tri-phasic scaffold composed of PLA/PCL-PLGA/Mg(OH)₂, which includes a cartilage layer, an osteochondral interface, and a bone layer. The scaffold was filled with Velvet antler polypeptides (VAP), and its characterization was assessed using compression testing, XRD, FTIR, SEM, fluorescence microscopy, and EDS. In vitro investigation demonstrated that the scaffold not only supported osteogenesis but also promoted chondrogenic differentiation of fibrocartilage stem cells (FCSCs). n vivo experiments showed that the tri-phasic PLA/PCL-PLGA/Mg(OH)2-VAP scaffold together with FCSC, when transplanted to animal models, increased the recovery of osteochondral defects. Those results demonstrate the promising future of illustrated tri-phasic PLA/PCL-PLGA/Mg(OH)2-VAP scaffold loaded with FCSCs as a new bone and cartilage tissue engineering approach for osteochondral defects treatment.

Tissue-resident memory T (TRM) cells infiltrating solid tumors could influence tumor progression and the response to immune therapies. However, the proportion and prognostic value of TRM cells in the bone marrow (BM) of patients with acute myeloid leukemia (AML) are unclear. In this study, we used flow cytometry to assay the phenotype of 49 BM samples from patients newly diagnosed with AML (ND-AML). We found that the BM CD8+ effector memory (TEM) cells highly expressed CD69 (CD8+ TRM-like T cells), and their percentage was significantly increased in patients with ND-AML compared with that in healthy individuals (HI). The high percentage of CD8+ TRM-like subset was associated with poor overall survival in our ND-AML cohort. The Kaplan–Meier Plotter database verified a significantly reduced survival rate among patients with high expression of CD8+ TRM-like T cell characteristic genes (CD8A, CD69, and TOX), especially the M4 and M5 subtypes. Phenotypic analysis revealed that the BM CD8+ TRM-like subpopulation exhibited exhausted T cell characteristics, but its high expression of CD27 and CD28 and low expression of CD57 suggested its high proliferative potential. The single-cell proteogenomic dataset confirmed the existence of TRM-like CD8+ T cells in the BM of patients with AML and verified the high expression of immune checkpoints and costimulatory molecules. In conclusion, we found that the accumulation of BM CD8+ TRM-like cells could be an immune-related survival prediction marker for patients with AML.

Recycled aggregate concrete, as a construction material, can be applied to structural components (beams, slabs, columns, and foundations), auxiliary components, and temporary facilities with strength grades ranging from C30 to C50. Today, the world is facing an increasing pressure of greenhouse gas emissions. Utilizing carbon dioxide for the curing of recycled aggregates contributes to achieving carbon reduction goals. Freeze damage can cause deterioration of mechanical properties and surface spalling of recycled aggregate concrete (RAC). This study investigated the influence of three different CO2 curing methods, standard CO2 curing (CC group), CO2 curing with pre-saturated Ca(OH)2 immersion (HH group), and whole specimen CO2 curing (CRR group), on the properties of RAC subjected to freeze-thaw cycles. The results revealed that all the three CO2 curing methods improved the peak stress and elastic modulus of RAC by 12.3–13.7 % and 19.2–31.5 %, respectively. The HH group suffered the least mass loss rate and experienced the most improvement in the relative dynamic modulus. The improvement in the RAC impermeability was in the descending order of HH group > CC group > CRR group. It should be pointed out that RAC with a lower initial water-cement (w/c) ratio exhibited better permeability resistance. Compared with the reference group, the CC, HH, and CRR groups showed a respective 49.0–58.7 %, 55.8–72.6 %, and 45.5–46.8 % reduction in the capillary water absorption coefficients after being subjected to different CO2 curing methods. Among the three groups, the HH group with a w/c ratio of 0.35 and 0.55 showed better resistance to chloride ion penetration (which was further reduced by 9.4–31.6 % and 19.2–41.1 %, respectively).

Abstract Laryngopharyngeal reflux disease (LPRD) is a condition characterized by the regurgitation of stomach and duodenal contents into the laryngopharynx, with variable and non-specific symptoms. Therefore, developing an accurate symptom scale for different regions is essential. Notably, the symptoms of “dryness and burning sensation in the laryngopharynx or mouth” are prevalent among the Chinese population but are often omitted from conventional symptom assessment scales, such as the Reflux Symptom Index (RSI) and Reflux Symptom Score-12 (RSS-12) scales. To address this gap, our study incorporated the symptoms into the RSI and RSS-12 scales, developing the RSI-10/RSS-13 scales. Afterward, we assessed the role of the new scale’s reliability (Cronbach’s α and test–retest reliability), construct validity (confirmatory factor analysis and confirmatory factor analysis), and diagnostic efficiency. Our study encompassed 479 participants (average = 39.5 ± 13.4 years, 242 female) and 91 (average = 34.01 ± 13.50 years, 44 female) completed 24 h MII-pH monitoring. The Cronbach’s α values of 0.80 and 0.82 for the RSI-10 and RSS-13 scales, respectively. RSI-10 and RSS-13 exhibited strong test–retest reliability (ICCs = 0.82–0.96) and diagnostic efficacy (AUC = 0.84–0.85). Furthermore, the factor analysis identified the RSS-13 and its three sub-scales (ear-nose-throat, digestive tract, respiratory tract) exhibited good to excellent structural validity (χ2/df = 1.95, P

Highlights A vanadium oxide (TMPA-VOH) is synthesized with trimethylphenylammonium cations chemically pre-inserted into hydrated vanadium oxide. The pre-intercalation of weakly polarized organic cations strategically utilizes both ionic and molecular pre-intercalation effects. TMPA-VOH, with modified crystal structure and morphology, increased V4+ content, and weakened electrostatic interactions between Zn2+ and the V-O lattice, demonstrates enhanced voltage, storage capacity, structural stability, and reaction kinetics.

In the working process of the tethered unmanned aerial vehicle (UAV), there is interference from the tethered cable, which can easily lead to the instability of the UAV. To solve the above problems, a method based on the Lyapunov exponent is proposed to analyze the stability of tethered cables for tethered UAVs. The dynamics equation of the UAV platform is established using the Euler–Poincare equation. The tension formula of the tethered cable is derived from the catenary theory and the principle of micro-segment equilibrium. Based on the Lyapunov exponential method, the stability changes of the tethered UAV in the takeoff, hovering, and landing stages are simulated and analyzed in a MATLAB environment. Prototype tests are carried out to prove the correctness of the simulation model and calculation conclusions. The results show that with an increase in the density of the tethered cable, the stability of the tethered UAV tends to decrease. At the same time, stability is affected by the density of the tethered cable more often during takeoff than during landing.

In order to investigate the interaction between aloe emodin and α-glucosidase, enzyme kinetics, ultraviolet (UV) spectroscopy, infrared (IR) spectroscopy and fluorescence spectroscopy were employed to investigate the inhibitory mechanism of aloe emodin on α-glucosidase and the synergistic effect of aloe emodin in combination with acarbose was also investigated in this study. The results showed that aloe emodin had better inhibitory effect on α-glucosidase as both a non-competitive and anti-competitive inhibitor when compared with acarbose. The results of UV spectroscopy indicated that a new complex was formed through the interaction between aloe emodin and α-glucosidase. The characteristic vibrations of amide groups in the IR spectra indicated that the structural conformation of α-glucosidase changed with the addition of aloe emodin. The results of fluorescence quenching experiments showed that the endogenous fluorescence of α-glucosidase was statically quenched by aloe emodin. In addition, it was also found that aloe emodin combined with acarbose had a synergistic inhibitory effect on α-glucosidase activity. This study provides an experimental basis for the future application of aloe emodin in health foods for regulating blood glucose.