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This work aimed to determine potential sites of contamination and transmission of SARS-CoV-2 during the COVID-19 pandemic in a public hospital in Culiacan, Sinaloa, México. The sampling was carried out from February to April 2022 of surfaces in the COVID areas and the Intensive Care Unit (ICU) of one public hospital in Culiacan, Sinaloa, Mexico. Samples were taken using a sterile sponge moistened with phosphate buffer. RNA extraction was performed with the Viral RNA mini kit (Qiagen), and the detection was performed with the CDC 2019-Novel Coronavirus kit. During the surveillance period in the public hospital, 11 samples were taken from surfaces. SARS-CoV-2 was found in 63.6 % of the samples. SARS-CoV-2 was absent from the ICUs; however, it was recovered in the COVID area of the public hospital (n = 7). The COVID area was the main point of contamination during the pandemic. On the other hand, it is notable that the virus was not detected outside this area, which can be associated with the adequate hospital containment measures adopted during the health emergency. [ABSTRACT FROM AUTHOR]