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Cell-based therapies represent the current frontier of biomedical innovations, with the technologies required underpinning treatments as broad as CAR-T cell therapies, stem cell treatments, genetic therapies and mRNA manufacture. A key bottleneck in the manufacturing process for each of these lies in the expansion of cells within a bioreactor vessel, requiring by far the greatest share of time for what are often time-critical therapies. While various designs, culture feeding and mixing methods are employed in these bioreactors, a common concern among manufacturers and researchers lies in whether shear stresses generated by culture media flow will damage cells and inhibit expansion. This study develops an analytical tool to link macro-scale measures of flow to risk of cell death using relationships with eddy size and dissipation rates, from eddies generated off flat surfaces. This analytical tool was then employed using computational fluid dynamics (CFD) to replicate a range of generic bioreactor geometries and flow conditions. We found that no combination of flow condition or design parameter was predicted by the tool to cause cell death within eddies, indicating negligible risk of cell death due to eddy formation within cell culture systems. While this requires experimental validation, and does not apply when cells are expanded using microcarriers, this tool nonetheless provides reassurance and accessible prediction of bioreactor design parameters that could result in cell death. Finally, our findings show that bioreactor design can be tailored such that the shear stress stimulation of cells can be selectively altered through small changes in flow rate.

Abstract Building upon the concept of mainstreaming social sciences within conservation, we consider their mainstreaming, and so integration, within UK environment policy and practice (EPP) organisations. The paper responds to increasing calls to recognise the essential role of social sciences in addressing global environmental crises across policy, practice and research. An actor-oriented approach was deployed, producing empirical information from a multi-stage, co-designed, collaborative study involving 19 social scientists from a range of EPP organisations, to understand how they experience the mainstreaming of social sciences. The findings contribute to debates about the politics of knowledge in organisational domains other than those focused on research, specifically EPP organisations. Evidence was found of recent positive changes in how social sciences are perceived, resourced and utilised within EPP, as well as examples of positive impact. However, although EPP organisations are recognising the opportunities that social sciences expertise brings, in practice social sciences still face barriers to effective integration. Many of the challenges faced by the social sciences within academic multi-discipline research (e.g., late, narrow, or selective enrolment) were also experienced in EPP organisations, along with some unique challenges. Informed by the findings, the paper proposes a set of integration indicators designed to assess organisational progress toward addressing the observed challenges. It is recommended that these indicators are employed at a strategic level by EPP organisations seeking to better integrate social sciences expertise into their work.

Transitioning out of elite sports can be a challenging time for athletes. To illuminate the gaps and opportunities in existing support systems and better understand which initiatives may have the greatest benefit in supporting athletes to transition out of elite sport, this study examined the lived experience of retired elite Australian athletes. Using a sequential mixed-methods approach, quantitative data were collected via a self-report online survey, while qualitative data were collected via semistructured interviews. In total 102 retired high-performance athletes (M=27.35, SD=7.25 years) who competed in an Olympic or Paralympic recognised sport at the national and/or international-level participated in the online survey, providing data across domains of well-being and athletic retirement. Eleven survey respondents opted in for the semistructured interview (M=28.9, SD=6.9 years) providing in-depth responses on their retirement experiences. Using partial least squares structural equation modelling (PLS-SEM), latent variables were identified from the survey data and associations between retirement support, retirement difficulties, retirement experiences, well-being and mental health were determined. Interview data were thematically analysed. The structural model had good predictive validity for all nine latent variables, describing positive and negative associations of retirement experiences, mental health and well-being. Building an identity outside of sport, planning for retirement, and having adaptive coping strategies positively impacted retirement experiences. Feeling behind in a life stage and an abrupt loss of athletic identity had a negative impact on retirement experiences. Implications for sports policymakers are discussed, including support strategies that could better assist athletes in successfully transitioning from elite sports.

Introduction/Purpose: The aim was to compare different bone marrow stimulation techniques and consequent fluid permeability of subchondral bone by assessing flow of radiopaque contrast agent using μCT image analysis and 3D modelling. Methods: Donated human tali specimens (n=12) were prepared by creating separate matched 10mm diameter chondral defects in each. Each defect underwent one of three surgical techniques: fine wire drilling, nanofracture or microfracture, addition of radiopaque contrast agent and imaged using a clinical μCT scanner. Using Slicer 3D software each μCT scan was segmented for bone and contrast agent regions in each surgical site of each sample. Each site was resolved into a cylinder and the ratio of segmented pixels of contrast agent against bone calculated. Results: μCT analysis indicated that 8/12 nanofracture regions demonstrated enhanced flow of contrast to at least the depth of the fracture site, with some additional lateral flow also observed. 8/12 microfracture regions demonstrated flow of contrast agent localised to the fracture site and preferential flow laterally. Only 1/12 samples with fine wire drilling demonstrated any fluid flow. In 11/12 samples that showed no permeation of contrast agent, a residual layer of contrast agent on the chondral surface was seen. Segmentation of each sample site showed a significant increase (n=12, p< 0.05) in fluid flow of the contrast agent in the nanofracture sites (11%) compared to microfracture (5%) and fine wire drilling (2%). Conclusion: Nanofracture showed significantly improved fluid permeability throughout the surrounding trabecular structure, when compared to microfracture and fine wire drilling. Microfracture allowed some fluid flow, but only confined to the immediate area around the fracture site, while fine wire drilling allows very little fluid flow at all. This study suggests that nanofracture should perhaps be the preferred mode of subchondral bone preparation for osteochondral lesions of the talus. Quantitative measurement of contrast permeability into talar subchondral bone by technique Nanofracture showed significantly improved fluid permeability throughout the surrounding trabecular structure, when compared to microfracture and fine wire drilling

Data now plays a central role in civic life and community practices. This has created a pressing need for new forms of translation and sense-making that can engage diverse publics. Research-based Theatre (RbT) has proven to be an effective approach to delivering qualitative data to community stakeholders. We extend this tradition by proposing “community-engaged data theatre”. This approach translates quantitative data into theatrical language to engage communities in deliberative conversations on relevant issues. Community-engaged data theatre requires bridging multiple disciplines and involves creating new definitions and shared vocabularies in discourses that formerly have had little overlap in meaning. In this article, we share key insights from our initial experiments in which we adapted quantitative and qualitative data to devise a pilot piece in collaboration with a local community partner. In this essay, we communicate our collaborative process in polyvocal, artistic form. We edit and adapt materials from our conversations and creative practices into scenes illustrating how we taught and learned from each other about data science, participatory modeling, material deliberation and Composition to pilot our lab’s first community-engaged data theatre prototype.

This article introduces a special issue on Tim Hetherington and conflict imagery, which is the result of an international research project led by the University of Leeds and the Imperial War Museum. The project aimed to understand Hetherington's work by engaging with his associates, contemporary photojournalists, scholars, and the public. The special issue explores themes such as humanitarian photography, military masculinity, and the changing landscape of image-making during conflict. It includes articles that analyze Hetherington's representations of Afghanistan, focusing on aesthetic themes, soldiers' experiences, historical context, and audience interactions. The article concludes by encouraging further research using the Tim Hetherington collection. [Extracted from the article]

War photography scholarship tends to focus attention on photographers' lives and the thematic content of images. This study shifts the spotlight onto how varied members of the public respond to a body of work, in this case the photography and filmmaking of Tim Hetherington (1970-2011), whose archive the Imperial War Museum (IWM) obtained in 2017. We discuss findings from a number of visually-led focus groups with refugees, veterans, military family members, photography students and museum volunteers. Together we examined his image-making process with the groups to discover how they discussed notions such as intimacy, masculinity, the 'good' conflict photograph, and ethical dilemmas. We argue that engaging with multiple public perspectives through visual elicitation methods enhances curatorial knowledge and understanding of image-making practices in this specific context, whilst also offering insights for national museums more broadly on how dialogue with diverse communities can enrich the handling and display of new collections. [ABSTRACT FROM AUTHOR]

• During Covid-19 people visited a wide range of nearby places to get away from everyday demands, without needing to travel. • People engaged with a wide range of activities in those places, but many activities were place dependent. • All place visits benefitted hedonic and eudemonic wellbeing, but outdoor activities were more beneficial than indoor activities. • Place and activity choices varied between people. Younger people and those living in urban areas visited less outdoor places. • To support wellbeing for all it is important to identify the variety of nearby places people visit and manage access and provision of such places. Being able to get away from everyday stressors and demands, even if close to home and just for a few minutes, is important for wellbeing. During the Covid-19 lockdown periods, people's ability to get away changed significantly. An increase in visits to nearby natural places is well documented. Little is known about other types of places people visited to get away. An online UK survey was conducted in 2020 (N = 850) investigating what places people visited to get away during the pandemic, what they did in those places, how place and activity choices were related to each other and to demographic variables, and to recalled hedonic and eudaimonic wellbeing during those visits. Participants visited a rich array of places and engaged in a variety of activities that supported their hedonic and eudaimonic wellbeing needs. Responses were grouped into four types of places (at home outdoors, at home indoors, away from home outdoors, and away from home indoors) and seven activity types (cognitive, walks, nature engagement, social activities, technology use, relaxing, and exercise). Place and activity choices were strongly linked. Visiting outdoor places was most beneficial for wellbeing (and most common), especially when it involved mindful engagement with nature (bird watching, gardening) or exercise. Staying indoors, engaging with technologies (computers, television) was least beneficial and more common among those with no degree or job, living in urban areas, and identifying as male. The findings demonstrate the importance of understanding place-activity interactions to support the wellbeing benefits derived from visits to places to get away. [ABSTRACT FROM AUTHOR]

Diabetic foot ulceration is linked to high amputation and mortality rates, with the substantial associated annual spend on the at-risk diabetic foot reflecting the intensive time and labour involved in treatment. Assessing plantar interactions and developing improved understanding of the formation pathways of diabetic ulceration is important to orthotic interventions and patient outcomes. Plantar skin surrogates which emulate the mechanical and tribological characteristics can help improve physical models of ulceration, reduce reliance on cadaveric use and inform more complex computational modelling approaches. The information available from existing studies to characterise plantar skin is limited, typically featuring ex-vivo representations of skin and subcutaneous tissue combined and given focus to shear studies with time dependency. The aim of this study is to improve understanding of plantar tissue mechanics by assessing the mechanical characteristics of plantar skin in two groups; (1) non-diabetic and (2) diabetic donors without the subcutaneous tissue attachment of previous work in this field. Digital image correlation was used to assess inherent skin pre-tension of the plantar rearfoot prior to dissection. Young's modulus, storage and loss moduli were tested for using tensile stress–strain failure analysis and tensile and compressive dynamic mechanical analysis, which was conducted on excised plantar rearfoot donor specimens for both disease state cohorts at frequencies reflecting those achieved in activities of daily living. Plantar skin thickness for donor specimens were comparable to values obtained using ultrasound acquired in vivo values. Median tensile storage and loss moduli, along with Young's modulus, was higher in the diabetic cohort. With a mean Young's modulus of 0.83 ± 0.49 MPa and 1.33 ± 0.43 MPa for non-diabetic and diabetic specimens respectively. Compressive studies showed consistency between cohorts for median storage and loss moduli. The outcomes from this study show mechanical characteristics of plantar skin without the involvement of subcuteanous tissues under reflective daily achieved loading regimes, showing differences in the non-diabetic and diabetic specimens trialled to support improved understanding of plantar tissue response under tribological interactions. [ABSTRACT FROM AUTHOR]