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We analyzed dog care practices among 1,006 owners in urban and rural areas in Uganda to identify the root causes of roaming. The dog owners were mostly male, aged 20–39 years, and low-income earners. Each household in urban areas had an average of 2.36 dogs (SD 3.35), and 2.35 dogs (SD 2.18) in rural areas. We determined that 16.1% of urban dogs and 29.4% of rural dogs roamed during the day, with numbers rising to 26.0% and 65.2% at night (p < 0.001), due to dogs being released at night for security purposes. Key drivers of roaming included inadequate confinement, particularly the lack of a secure enclosure around homes, underfeeding and abandonment. These issues were linked to poverty, low education and poor attitudes. Our findings provide a foundation for developing tailored strategies to control free-roaming dogs (FRD) in Uganda. Short-term solutions include implementing fines for owners who allow their dogs to roam, subsidized dog sterilization, and public education programs. Long-term strategies should involve policies on dog ownership, registration, breeding, and research to develop evidence-based control measures for FRD. [ABSTRACT FROM AUTHOR]

Domestic dogs ( Canis lupus familiaris ) live with humans, frequently contact other animals and may serve as intermediary hosts for the transmission of viruses. Free-roaming dogs, which account for over 70% of the world's domestic dog population, may pose a particularly high risk in this regard. We conducted an epidemiological study of dog viromes in three locations in Uganda, representing low, medium and high rates of contact with wildlife, ranging from dogs owned specifically for traditional hunting in a biodiversity and disease 'hotspot' to pets in an affluent suburb. We quantified rates of contact between dogs and wildlife through owner interviews and conducted canine veterinary health assessments. We then applied broad-spectrum viral metagenomics to blood plasma samples, from which we identified 46 viruses, 44 of which were previously undescribed, in three viral families, Sedoreoviridae , Parvoviridae and Anelloviridae . All 46 viruses (100 %) occurred in the high-contact population of dogs compared to 63 % and 39 % in the medium- and low-contact populations, respectively. Viral prevalence ranged from 2.1 % to 92.0 % among viruses and was highest, on average, in the high-contact population (22.3 %), followed by the medium-contact (12.3 %) and low-contact (4.8 %) populations. Viral richness (number of viruses per dog) ranged from 0 to 27 and was markedly higher, on average, in the high-contact population (10.2) than in the medium-contact (5.7) or low-contact (2.3) populations. Viral richness was strongly positively correlated with the number of times per year that a dog was fed wildlife and negatively correlated with the body condition score, body temperature and packed cell volume. Viral abundance (cumulative normalized metagenomic read density) varied 124-fold among dogs and was, on average, 4.1-fold higher and 2.4-fold higher in the high-contact population of dogs than in the low-contact or medium-contact populations, respectively. Viral abundance was also strongly positively correlated with the number of times per year that a dog was fed wildlife, negatively correlated with packed cell volume and positively correlated with white blood cell count. These trends were driven by nine viruses in the family Anelloviridae , genus Thetatorquevirus , and by one novel virus in the family Sedoreoviridae , genus Orbivirus . The genus Orbivirus contains zoonotic viruses and viruses that dogs can acquire through ingestion of infected meat. Overall, our findings show that viral prevalence, richness and abundance increased across a gradient of contact between dogs and wildlife and that the health status of the dog modified viral infection. Other ecological, geographic and social factors may also have contributed to these trends. Our finding of a novel orbivirus in dogs with high wildlife contact supports the idea that free-roaming dogs may serve as intermediary hosts for viruses of medical importance to humans and other animals.