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1. Fluorogenic cell surface glycan labelling with fluorescence molecular rotor dyes and nucleic acid stains.

Author

Koçak A, Homer AK, Feida A, Telschow F, Gorenflos López JL, Baydaroğlu C, Gradzielski M, Hackenberger CPR, Alexiev U, and Seitz O

Subjects
Humans, Polysaccharides chemistry, Nucleic Acids chemistry, Nucleic Acids analysis, Carbocyanines chemistry, Staining and Labeling methods, Fluorescence, Quinolines chemistry, Benzothiazoles chemistry, Fluorescent Dyes chemistry
Abstract

We show that covalent labelling of sialic acids on live cell surfaces or mucin increases the fluorescence of the fluorescence molecular rotors (FMRs) CCVJ, Cy3 and thioazole orange, enabling wash-free imaging of cell surfaces. Dual labelling with an FMR and an environmentally insensitive dye allows detection of changes that occur, for example, when cross-linking is altered.

Published
2024
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