37 results on '"Anichini A"'
Search Results
2. Invisible to Machines: Designing AI that Supports Vision Work in Radiology
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Anichini, Giulia, Natali, Chiara, and Cabitza, Federico
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- 2024
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3. Tumor-derived GLI1 promotes remodeling of the immune tumor microenvironment in melanoma
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Alessandro Giammona, Chiara De Vellis, Enrica Crivaro, Luisa Maresca, Roberta Amoriello, Federica Ricci, Giulia Anichini, Silvia Pietrobono, David R. Pease, Martin E. Fernandez-Zapico, Clara Ballerini, and Barbara Stecca
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Melanoma ,GLI1 ,CX3CL1 ,Immune escape ,Myeloid-derived suppressor cells ,Dendritic cells ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Melanoma progression is based on a close interaction between cancer cells and immune cells in the tumor microenvironment (TME). Thus, a better understanding of the mechanisms controlling TME dynamics and composition will help improve the management of this dismal disease. Work from our and other groups has reported the requirement of an active Hedgehog-GLI (HH-GLI) signaling for melanoma growth and stemness. However, the role of the downstream GLI1 transcription factor in melanoma TME remains largely unexplored. Methods The immune-modulatory activity of GLI1 was evaluated in a syngeneic B16F10 melanoma mouse model assessing immune populations by flow cytometry. Murine polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) were differentiated from bone marrow cells and their immunosuppressive ability was assessed by inhibition of T cells. Conditioned media (CM) from GLI1-overexpressing mouse melanoma cells was used to culture PMN-MDSCs, and the effects of CM were evaluated by Transwell invasion assay and T cell inhibition. Cytokine array analysis, qPCR and chromatin immunoprecipitation were performed to explore the regulation of CX3CL1 expression by GLI1. Human monocyte-derived dendritic cells (moDCs) were cultured in CM from GLI1-silenced patient-derived melanoma cells to assess their activation and recruitment. Blocking antibodies anti-CX3CL1, anti-CCL7 and anti-CXCL8 were used for in vitro functional assays. Results Melanoma cell-intrinsic activation of GLI1 promotes changes in the infiltration of immune cells, leading to accumulation of immunosuppressive PMN-MDSCs and regulatory T cells, and to decreased infiltration of dendric cells (DCs), CD8 + and CD4 + T cells in the TME. In addition, we show that ectopic expression of GLI1 in melanoma cells enables PMN-MDSC expansion and recruitment, and increases their ability to inhibit T cells. The chemokine CX3CL1, a direct transcriptional target of GLI1, contributes to PMN-MDSC expansion and recruitment. Finally, silencing of GLI1 in patient-derived melanoma cells promotes the activation of human monocyte-derived dendritic cells (moDCs), increasing cytoskeleton remodeling and invasion ability. This phenotype is partially prevented by blocking the chemokine CCL7, but not CXCL8. Conclusion Our findings highlight the relevance of tumor-derived GLI1 in promoting an immune-suppressive TME, which allows melanoma cells to evade the immune system, and pave the way for the design of new combination treatments targeting GLI1.
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- 2024
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4. Neuronal and Mixed Neuronal-Glial Tumors
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Mahoney, Dominic E., Anichini, Giulio, O’Neill, Kevin, Delawan, Maliya, Ma, Li, Hoz, Samer S., editor, Atallah, Oday, editor, Ma, Li, editor, Aljuboori, Zaid, editor, Sharma, Mayur, editor, Ismail, Mustafa, editor, and Delawan, Maliya, editor
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- 2024
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5. Epigenetic remodeling to improve the efficacy of immunotherapy in human glioblastoma: pre-clinical evidence for development of new immunotherapy approaches
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Maria Fortunata Lofiego, Francesca Piazzini, Francesca Pia Caruso, Francesco Marzani, Laura Solmonese, Emma Bello, Fabrizio Celesti, Maria Claudia Costa, Teresa Noviello, Roberta Mortarini, Andrea Anichini, Michele Ceccarelli, Sandra Coral, Anna Maria Di Giacomo, Michele Maio, Alessia Covre, and The EPigenetic Immune-oncology Consortium Airc (EPICA) investigators
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Glioblastoma ,Brain metastases ,Immunotherapy ,DNA hypomethylating agent ,Melanoma ,Medicine - Abstract
Abstract Background Glioblastoma multiforme (GBM) is a highly aggressive primary brain tumor, that is refractory to standard treatment and to immunotherapy with immune-checkpoint inhibitors (ICI). Noteworthy, melanoma brain metastases (MM-BM), that share the same niche as GBM, frequently respond to current ICI therapies. Epigenetic modifications regulate GBM cellular proliferation, invasion, and prognosis and may negatively regulate the cross-talk between malignant cells and immune cells in the tumor milieu, likely contributing to limit the efficacy of ICI therapy of GBM. Thus, manipulating the tumor epigenome can be considered a therapeutic opportunity in GBM. Methods Microarray transcriptional and methylation profiles, followed by gene set enrichment and IPA analyses, were performed to study the differences in the constitutive expression profiles of GBM vs MM-BM cells, compared to the extracranial MM cells and to investigate the modulatory effects of the DNA hypomethylating agent (DHA) guadecitabine among the different tumor cells. The prognostic relevance of DHA-modulated genes was tested by Cox analysis in a TCGA GBM patients’ cohort. Results The most striking differences between GBM and MM-BM cells were found to be the enrichment of biological processes associated with tumor growth, invasion, and extravasation with the inhibition of MHC class II antigen processing/presentation in GBM cells. Treatment with guadecitabine reduced these biological differences, shaping GBM cells towards a more immunogenic phenotype. Indeed, in GBM cells, promoter hypomethylation by guadecitabine led to the up-regulation of genes mainly associated with activation, proliferation, and migration of T and B cells and with MHC class II antigen processing/presentation. Among DHA-modulated genes in GBM, 7.6% showed a significant prognostic relevance. Moreover, a large set of immune-related upstream-regulators (URs) were commonly modulated by DHA in GBM, MM-BM, and MM cells: DHA-activated URs enriched for biological processes mainly involved in the regulation of cytokines and chemokines production, inflammatory response, and in Type I/II/III IFN-mediated signaling; conversely, DHA-inhibited URs were involved in metabolic and proliferative pathways. Conclusions Epigenetic remodeling by guadecitabine represents a promising strategy to increase the efficacy of cancer immunotherapy of GBM, supporting the rationale to develop new epigenetic-based immunotherapeutic approaches for the treatment of this still highly deadly disease.
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- 2024
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6. L’Ateneo e il mondo della scienza
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Dei, Luigi, primary, Dominici, Daniele, additional, Mazzoni, Massimo, additional, Droescher, Cora Ariane, additional, Benvenuti, Marco, additional, Bruni, Paola, additional, Turano, Paola, additional, Anichini, Giuseppe, additional, Antonini, Samuele, additional, Barletti, Luigi, additional, Brugnano, Luigi, additional, Fusi, Lorenzo, additional, Gavagna, Veronica, additional, Rampichini, Carla, additional, Salvini, Antonella, additional, and Lo Nostro, Pierandrea, additional
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- 2024
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7. Identifying losers: Automatic identification of growth-stunted salmon in aquaculture using computer vision
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Kana Banno, Filipe Marcel Fernandes Gonçalves, Clara Sauphar, Marianna Anichini, Aline Hazelaar, Linda Helen Sperre, Christian Stolz, Grete Hansen Aas, Lars Christian Gansel, and Ricardo da Silva Torres
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Aquaculture ,Growth-stunted fish ,Fish welfare ,Machine learning ,Object detection ,Object classification ,Cybernetics ,Q300-390 ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
During the production of salmonids in aquaculture, it is common to observe growth-stunted individuals. The cause for the so-called “loser fish syndrome” is unclear, which needs further investigation. Here, we present and compare computer vision systems for the automatic detection and classification of loser fish in Atlantic salmon images taken in sea cages. We evaluated two end-to-end approaches (combined detection and classification) based on YoloV5 and YoloV7, and a two-stage approach based on transfer learning for detection and an ensemble of classifiers (e.g., linear perception, Adaline, C-support vector, K-nearest neighbours, and multi-layer perceptron) for classification. To our knowledge, the use of an ensemble of classifiers, considering consolidated classifiers proposed in the literature, has not been applied to this problem before. Classification entailed the assigning of every fish to a healthy and a loser class. The results of the automatic classification were compared to the reliability of human classification. The best-performing computer vision approach was based on YoloV7, which reached a precision score of 86.30%, a recall score of 71.75%, and an F1 score of 78.35%. YoloV5 presented a precision of 79.7%, while the two-stage approach reached a precision of 66.05%. Human classification had a substantial agreement strength (Fleiss’ Kappa score of 0.68), highlighting that evaluation by a human is subjective. Our proposed automatic detection and classification system will enable farmers and researchers to follow the abundance of losers throughout the production period. We provide our dataset of annotated salmon images for further research.
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- 2024
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8. Development of a microwave sensor for the non-invasive detection of plant responses to water stress: A practical application on maize (Zea mays L.)
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Lazzoni, Valeria, Brizi, Danilo, Staglianò, Nicolina, Giordano, Cristiana, Pecoraro, Elisa, Anichini, Monica, Ugolini, Francesca, Bindi, Marco, Argenti, Giovanni, Monorchio, Agostino, and Rossi, Riccardo
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- 2024
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9. Treatment intensification following glucagon-like peptide-1 receptor agonist in type 2 diabetes: Comparative effectiveness analyses between free vs. fixed combination of GLP-1 RA and basal insulin. RESTORE-G real-world study
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Gabellieri, Enrico, Tortato, Elena, Rabini, Rosa Anna, Crazzolara, Dalia, Lucibelli, Luigi, Aragiusto, Concetta, Panzolato, Gianluigi, Di Mauro, Maurizio, Del Buono, Andrea, Placentino, Giuseppe, Di Cianni, Graziano, Brandoni, Gabriele, Fazion, Stefano, Gregori, Giovanna, Di Benedetto, Antonino, De Riva, Carlo, Terracciano, Annamaria, Zenari, Luciano, Cavalot, Franco, Porcellati, Francesca, Anichini, Roberto, Citro, Giuseppe, D'Angelo, Paola, Arca, Marcello, Morviducci, Lelio, Montani, Valeria, Fiorentini, Paolo, Candido, Riccardo, Nicolucci, Antonio, Larosa, Monica, Rossi, Maria Chiara, and Napoli, Raffaele
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- 2024
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10. ModeLLer - Enabling End-Users to Model Systems: a Case Study in Digital Agriculture.
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Chiara Mannari, Elisa Anichini, Manlio Bacco, Alessio Ferrari 0001, Tommaso Turchi, and Alessio Malizia
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- 2024
11. Expanded vision for the spatial distribution of Atlantic salmon in sea cages
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Banno, Kana, Gao, Sihan, Anichini, Marianna, Stolz, Christian, Tuene, Stig Atle, and Gansel, Lars Christian
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- 2024
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12. Identifying losers: Automatic identification of growth-stunted salmon in aquaculture using computer vision
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Banno, Kana, Gonçalves, Filipe Marcel Fernandes, Sauphar, Clara, Anichini, Marianna, Hazelaar, Aline, Sperre, Linda Helen, Stolz, Christian, Aas, Grete Hansen, Gansel, Lars Christian, and da Silva Torres, Ricardo
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- 2024
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13. Tensions et convergences dans la conception de nouveaux outils d’intelligence artificielle pour l’oncologie : le cas de la radiomique
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Giulia Anichini and Léo Mignot
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predictive medicine ,cancer ,Artificial intelligence ,professional standards ,radiomics ,Anthropology ,GN1-890 - Abstract
The emerging field of radiomics aims to extract quantitative information from medical images. This advanced analysis technique seeks to identify specific biomarkers that can improve patient categorization and care. In oncology, clinicians collaborate with experts in image processing to design new predictive models based on artificial intelligence. This article demonstrates how technicizing diagnostic and prognostic tools has led both to a convergence of interests in this new field – that allows for an accumulation of scientific capital – and to tensions affecting, among other things, the criteria involved in the validation of technologies. In particular, the performance metrics used by researchers do not allow clinicians to measure their clinical usefulness, which is judged by the context of use. Therefore, various standards are applied to evaluate these new imaging biomarkers and their success depends on the articulation between medical and computational knowledge.
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- 2024
14. Antibody dependent cellular cytotoxicity-inducing anti-EGFR antibodies as effective therapeutic option for cutaneous melanoma resistant to BRAF inhibitors
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Elena Muraro, Barbara Montico, Benedict Lum, Francesca Colizzi, Giorgio Giurato, Annamaria Salvati, Roberto Guerrieri, Aurora Rizzo, Elisa Comaro, Vincenzo Canzonieri, Andrea Anichini, Michele Del Vecchio, Roberta Mortarini, Massimo Milione, Alessandro Weisz, Maria Antonietta Pizzichetta, Fiona Simpson, Riccardo Dolcetti, Elisabetta Fratta, and Luca Sigalotti
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cutaneous melanoma ,BRAF ,drug resistance ,receptor tyrosine kinases ,antibody dependent cell cytotoxicity ,Immunologic diseases. Allergy ,RC581-607 - Abstract
IntroductionAbout 50% of cutaneous melanoma (CM) patients present activating BRAF mutations that can be effectively targeted by BRAF inhibitors (BRAFi). However, 20% of CM patients exhibit intrinsic drug resistance to BRAFi, while most of the others develop adaptive resistance over time. The mechanisms involved in BRAFi resistance are disparate and globally seem to rewire the cellular signaling profile by up-regulating different receptor tyrosine kinases (RTKs), such as the epidermal growth factor receptor (EGFR). RTKs inhibitors have not clearly demonstrated anti-tumor activity in BRAFi resistant models. To overcome this issue, we wondered whether the shared up-regulated RTK phenotype associated with BRAFi resistance could be exploited by using immune weapons as the antibody-dependent cell cytotoxicity (ADCC)-mediated effect of anti-RTKs antibodies, and kill tumor cells independently from the mechanistic roots.Methods and resultsBy using an in vitro model of BRAFi resistance, we detected increased membrane expression of EGFR, both at mRNA and protein level in 4 out of 9 BRAFi-resistant (VR) CM cultures as compared to their parental sensitive cells. Increased EGFR phosphorylation and AKT activation were observed in the VR CM cultures. EGFR signaling appeared dispensable for maintaining resistance, since small molecule-, antibody- and CRISPR-targeting of EGFR did not restore sensitivity of VR cells to BRAFi. Importantly, immune-targeting of EGFR by the anti-EGFR antibody cetuximab efficiently and specifically killed EGFR-expressing VR CM cells, both in vitro and in humanized mouse models in vivo, triggering ADCC by healthy donors’ and patients’ peripheral blood cells. ConclusionOur data demonstrate the efficacy of immune targeting of RTKs expressed by CM relapsing on BRAFi, providing the proof-of-concept supporting the assessment of anti-RTK antibodies in combination therapies in this setting. This strategy might be expected to concomitantly trigger the crosstalk of adaptive immune response leading to a complementing T cell immune rejection of tumors.
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- 2024
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15. Long-Term Conservation for the Safeguard of Abies nebrodensis: An Endemic and Endangered Species of Sicily
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Carla Benelli, Waed Tarraf, Tolga İzgü, Monica Anichini, Cecilia Faraloni, Maria Cristina Salvatici, Nourhene Jouini, Maria Antonietta Germanà, Roberto Danti, and Maurizio Lambardi
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Sicilian fir ,cryopreservation ,cryobank ,pollen ,zygotic embryos ,embryogenic callus ,Botany ,QK1-989 - Abstract
The combined approaches between ex situ and in situ conservation are of great importance for threatened species in urgent need of protection. This study aims to develop concrete actions to preserve the relic of 30 adult trees of the Sicilian fir (Abies nebrodensis) from extinction using long-term germplasm conservation in liquid nitrogen (LN, −196 °C). Pollen grains were collected, and their moisture content (MC) was measured. Then, viability (2,3,5-tryphenyl tetrazolium chloride, TTC), in vitro germinability, and enzymatic antioxidant activity (ascorbate peroxidase, APX; catalase, CAT) were evaluated before and after cryopreservation. Seeds collected from mature cones underwent X-ray analysis, and only full seeds were used to excise the zygotic embryos (ZEs) for cryopreservation. The MC percentage of ZEs was determined, and then they were plunged in LN with (+PVS2) or without (−PVS2) Plant Vitrification Solution 2; untreated ZEs were used as a control. Viability (TTC test) and in vitro germination were assessed for all ZEs (+PVS2, −PVS2, and control). Embryogenic callus (EC) lines obtained from mature ZEs were cryopreserved applying the ‘encapsulation-dehydration’ technique. This study has allowed, after optimizing cryopreservation protocols for pollen, ZEs, and EC of A. nebrodensis, to establish the first cryobank of this endangered species in Polizzi Generosa (Palermo, Italy), inside the ‘Madonie Regional Park’. The strategy developed for Sicilian fir conservation will pave the way for similar initiatives for other critically endangered conifer species.
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- 2024
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16. Hyperspectral and multispectral imaging in neurosurgery: a systematic literature review and meta-analysis
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Anichini, Giulio, Leiloglou, Maria, Hu, Zepeng, O'Neill, Kevin, and Daniel Elson
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- 2024
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17. Deletion of 82–85 N-Terminal Residues in SARS-CoV-2 Nsp1 Restricts Virus Replication
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Gianni Gori Savellini, Gabriele Anichini, Fabrizio Manetti, Claudia Immacolata Trivisani, and Maria Grazia Cusi
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Nsp1 ,attenuated viruses ,transcription and translation ,host shutdown ,leader sequence ,SARS-CoV-2 ,Microbiology ,QR1-502 - Abstract
Non-structural protein 1 (Nsp1) represents one of the most crucial SARS-CoV-2 virulence factors by inhibiting the translation of host mRNAs and promoting their degradation. We selected naturally occurring virus lineages with specific Nsp1 deletions located at both the N- and C-terminus of the protein. Our data provide new insights into how Nsp1 coordinates these functions on host and viral mRNA recognition. Residues 82–85 in the N-terminal part of Nsp1 likely play a role in docking the 40S mRNA entry channel, preserving the inhibition of host gene expression without affecting cellular mRNA decay. Furthermore, this domain prevents viral mRNAs containing the 5′-leader sequence to escape translational repression. These findings support the presence of distinct domains within the Nsp1 protein that differentially modulate mRNA recognition, translation and turnover. These insights have implications for the development of drugs targeting viral proteins and provides new evidences of how specific mutations in SARS-CoV-2 Nsp1 could attenuate the virus.
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- 2024
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18. Treatment intensification following glucagon‐like peptide‐1 receptor agonists in type 2 diabetes: Comparative effectiveness analyses between different basal insulins. RESTORE‐G real‐world study.
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Napoli, Raffaele, Nicolucci, Antonio, Larosa, Monica, Rossi, Maria Chiara, Candido, Riccardo, Aragiusto, Concetta, Arca, Marcello, Anichini, Roberto, Brandoni, Gabriele, Cavalot, Franco, Citro, Giuseppe, Crazzolara, Dalia, D'Angelo, Paola, Del Buono, Andrea, De Riva, Carlo, Di Benedetto, Antonino, Di Cianni, Graziano, Di Mauro, Maurizio, Fazion, Stefano, and Fiorentini, Paolo
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TYPE 2 diabetes ,INSULIN receptors ,PROPENSITY score matching ,ELECTRONIC health records ,BLOOD sugar - Abstract
Aim: To compare the effectiveness of different basal insulins (BI) prescribed as an add‐on to or switch from glucagon‐like peptide‐1 receptor agonist (GLP‐1 RA) therapy. Materials and Methods: Retrospective, real‐world data from electronic medical records of 32 Italian diabetes clinics were used, after propensity score adjustment, to compare effectiveness after 6 months of treatment with second‐ versus first‐generation BI (2BI vs. 1BI) or glargine 300 U/ml versus degludec 100 U/ml (Gla‐300 vs. Deg‐100), when added to (ADD‐ON) or in substitution of (SWITCH) GLP‐1 RA. Only comparisons, including a minimum of 100 patients per group, were performed to ensure adequate robustness of the analyses. Results: In the ADD‐ON cohort (N = 700), greater benefits of 2BI versus 1BI were found in glycated haemoglobin {HbA1c; estimated mean difference: −0.32% [95% confidence interval (CI) −0.62; −0.02]; p =.04} and fasting blood glucose [FBG; −20.73 mg/dl (95% CI −35.62; −5.84); p =.007]. In the SWITCH cohort (N = 2097), greater benefits of 2BI versus 1BI were found in HbA1c [−0.22% (95% CI −0.42; −0.02); p =.03], FBG [−10.15 mg/dl (95% CI −19.04; −1.26); p =.03], and body weight [−0.67 kg (95% CI −1.30; −0.04); p =.04]. In the SWITCH cohort starting 2BI (N = 688), marked differences in favour of Gla‐300 versus Deg‐100 were documented in HbA1c [−0.89% (95% CI −1.26; −0.52); p <.001] and FBG [−17.89 mg/dl (95% CI −32.45; −3.33); p =.02]. Using propensity score matching as a sensitivity analysis, the benefit on HbA1c was confirmed [−0.55% (95% CI –1.02; −0.08); p =.02]. BI titration was suboptimal in all examined cohorts. Conclusions: 2BI are a valuable option to intensify GLP‐1 RA therapy. Switching to Gla‐300 versus Deg‐100 was associated with greater HbA1c improvement. [ABSTRACT FROM AUTHOR]
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- 2024
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19. SARS-CoV-2 JN.1 variant evasion of IGHV3-53/3-66 B cell germlines.
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Paciello, Ida, Maccari, Giuseppe, Pierleoni, Giulio, Perrone, Federica, Realini, Giulia, Troisi, Marco, Anichini, Gabriele, Cusi, Maria Grazia, Rappuoli, Rino, and Andreano, Emanuele
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SARS-CoV-2 ,IMMUNOGLOBULIN heavy chains ,IMMUNE response ,SARS-CoV-2 Omicron variant ,BREAKTHROUGH infections - Abstract
The severe acute respiratory syndrome coronavirus 2 variant JN.1 recently emerged as the dominant variant despite having only one amino acid change on the spike (S) protein receptor binding domain (RBD) compared with the ancestral BA.2.86, which never represented more than 5% of global variants. To define at the molecular level the JN.1 ability to spread globally, we interrogated a panel of 899 neutralizing human monoclonal antibodies. Our data show that the single leucine-455–to–serine mutation in the JN.1 spike protein RBD unleashed the global spread of JN.1, likely occurring by elimination of more than 70% of the neutralizing antibodies mediated by IGHV3-53/3-66 germlines. However, the resilience of class 3 antibodies with low neutralization potency but strong Fc functions may explain the absence of JN.1 severe disease. Editor's summary: New variants of SARS-CoV-2 continue to emerge and enable viral immune evasion. Using a panel of 899 human monoclonal neutralizing antibodies (nAbs), Paciello et al. examined antibody neutralization activity and effector functions against the recently dominant JN.1 variant. Compared with the ancestral BA.2.86 strain, around two-thirds of nAbs lost neutralization activity against JN.1, including most derived from the immunoglobulin heavy chain V gene (IGHV) 3-53 and 3-66 germlines. The remaining nAbs were only detected in individuals with a history of multiple vaccinations and Omicron breakthrough infections. Among nAbs that could bind the JN.1 spike protein, Fc functions were largely retained and primarily driven by class 3 antibodies that bind outside of the ACE2 binding region, possibly explaining the absence of JN.1 severe disease despite strong evasion of class 1/2 nAbs. —Claire Olingy [ABSTRACT FROM AUTHOR]
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- 2024
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20. Tumor-derived GLI1 promotes remodeling of the immune tumor microenvironment in melanoma.
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Giammona, Alessandro, De Vellis, Chiara, Crivaro, Enrica, Maresca, Luisa, Amoriello, Roberta, Ricci, Federica, Anichini, Giulia, Pietrobono, Silvia, Pease, David R., Fernandez-Zapico, Martin E., Ballerini, Clara, and Stecca, Barbara
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Background: Melanoma progression is based on a close interaction between cancer cells and immune cells in the tumor microenvironment (TME). Thus, a better understanding of the mechanisms controlling TME dynamics and composition will help improve the management of this dismal disease. Work from our and other groups has reported the requirement of an active Hedgehog-GLI (HH-GLI) signaling for melanoma growth and stemness. However, the role of the downstream GLI1 transcription factor in melanoma TME remains largely unexplored. Methods: The immune-modulatory activity of GLI1 was evaluated in a syngeneic B16F10 melanoma mouse model assessing immune populations by flow cytometry. Murine polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) were differentiated from bone marrow cells and their immunosuppressive ability was assessed by inhibition of T cells. Conditioned media (CM) from GLI1-overexpressing mouse melanoma cells was used to culture PMN-MDSCs, and the effects of CM were evaluated by Transwell invasion assay and T cell inhibition. Cytokine array analysis, qPCR and chromatin immunoprecipitation were performed to explore the regulation of CX3CL1 expression by GLI1. Human monocyte-derived dendritic cells (moDCs) were cultured in CM from GLI1-silenced patient-derived melanoma cells to assess their activation and recruitment. Blocking antibodies anti-CX3CL1, anti-CCL7 and anti-CXCL8 were used for in vitro functional assays. Results: Melanoma cell-intrinsic activation of GLI1 promotes changes in the infiltration of immune cells, leading to accumulation of immunosuppressive PMN-MDSCs and regulatory T cells, and to decreased infiltration of dendric cells (DCs), CD8 + and CD4 + T cells in the TME. In addition, we show that ectopic expression of GLI1 in melanoma cells enables PMN-MDSC expansion and recruitment, and increases their ability to inhibit T cells. The chemokine CX3CL1, a direct transcriptional target of GLI1, contributes to PMN-MDSC expansion and recruitment. Finally, silencing of GLI1 in patient-derived melanoma cells promotes the activation of human monocyte-derived dendritic cells (moDCs), increasing cytoskeleton remodeling and invasion ability. This phenotype is partially prevented by blocking the chemokine CCL7, but not CXCL8. Conclusion: Our findings highlight the relevance of tumor-derived GLI1 in promoting an immune-suppressive TME, which allows melanoma cells to evade the immune system, and pave the way for the design of new combination treatments targeting GLI1. [ABSTRACT FROM AUTHOR]
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- 2024
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21. Challenges with segmenting intraoperative ultrasound for brain tumours.
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Weld, Alistair, Dixon, Luke, Anichini, Giulio, Patel, Neekhil, Nimer, Amr, Dyck, Michael, O'Neill, Kevin, Lim, Adrian, Giannarou, Stamatia, and Camp, Sophie
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MAGNETIC resonance imaging ,BRAIN tumors ,BRAIN damage ,ULTRASONIC imaging ,NEUROSURGEONS - Abstract
Objective - Addressing the challenges that come with identifying and delineating brain tumours in intraoperative ultrasound. Our goal is to both qualitatively and quantitatively assess the interobserver variation, amongst experienced neuro-oncological intraoperative ultrasound users (neurosurgeons and neuroradiologists), in detecting and segmenting brain tumours on ultrasound. We then propose that, due to the inherent challenges of this task, annotation by localisation of the entire tumour mass with a bounding box could serve as an ancillary solution to segmentation for clinical training, encompassing margin uncertainty and the curation of large datasets. Methods - 30 ultrasound images of brain lesions in 30 patients were annotated by 4 annotators - 1 neuroradiologist and 3 neurosurgeons. The annotation variation of the 3 neurosurgeons was first measured, and then the annotations of each neurosurgeon were individually compared to the neuroradiologist's, which served as a reference standard as their segmentations were further refined by cross-reference to the preoperative magnetic resonance imaging (MRI). The following statistical metrics were used: Intersection Over Union (IoU), Sørensen-Dice Similarity Coefficient (DSC) and Hausdorff Distance (HD). These annotations were then converted into bounding boxes for the same evaluation. Results - There was a moderate level of interobserver variance between the neurosurgeons [ I o U : 0.789 , D S C : 0.876 , H D : 103.227 ] and a larger level of variance when compared against the MRI-informed reference standard annotations by the neuroradiologist, mean across annotators [ I o U : 0.723 , D S C : 0.813 , H D : 115.675 ] . After converting the segments to bounding boxes, all metrics improve, most significantly, the interquartile range drops by [ I o U : 37 % , D S C : 41 % , H D : 54 % ] . Conclusion - This study highlights the current challenges with detecting and defining tumour boundaries in neuro-oncological intraoperative brain ultrasound. We then show that bounding box annotation could serve as a useful complementary approach for both clinical and technical reasons. [ABSTRACT FROM AUTHOR]
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- 2024
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22. Deletion of 82–85 N-Terminal Residues in SARS-CoV-2 Nsp1 Restricts Virus Replication
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Gori Savellini, Gianni, primary, Anichini, Gabriele, additional, Manetti, Fabrizio, additional, Trivisani, Claudia Immacolata, additional, and Cusi, Maria Grazia, additional
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- 2024
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23. Antibody dependent cellular cytotoxicity-inducing anti-EGFR antibodies as effective therapeutic option for cutaneous melanoma resistant to BRAF inhibitors
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Muraro, Elena, primary, Montico, Barbara, additional, Lum, Benedict, additional, Colizzi, Francesca, additional, Giurato, Giorgio, additional, Salvati, Annamaria, additional, Guerrieri, Roberto, additional, Rizzo, Aurora, additional, Comaro, Elisa, additional, Canzonieri, Vincenzo, additional, Anichini, Andrea, additional, Del Vecchio, Michele, additional, Mortarini, Roberta, additional, Milione, Massimo, additional, Weisz, Alessandro, additional, Pizzichetta, Maria Antonietta, additional, Simpson, Fiona, additional, Dolcetti, Riccardo, additional, Fratta, Elisabetta, additional, and Sigalotti, Luca, additional
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- 2024
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24. Toward Safe and Collaborative Robotic Ultrasound Tissue Scanning in Neurosurgery
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Dyck, Michael, primary, Weld, Alistair, additional, Klodmann, Julian, additional, Kirst, Alexander, additional, Dixon, Luke, additional, Anichini, Giulio, additional, Camp, Sophie, additional, Albu-Schäffer, Alin, additional, and Giannarou, Stamatia, additional
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- 2024
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25. Chapter L’Ateneo e il mondo della scienza
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Dei, Luigi, Dominici, Daniele, Mazzoni, Massimo, Droescher, Cora Ariane, Benvenuti, Marco, Bruni, Paola, Turano, Paola, Anichini, Giuseppe, Antonini, Samuele, Barletti, Luigi, Brugnano, Luigi, Fusi, Lorenzo, Gavagna, Veronica, Rampichini, Carla, Salvini, Antonella, and Lo Nostro, Pierandrea
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physical technologies ,natural history collections ,biochemistry ,mathematical sciences ,conservation science ,thema EDItEUR::N History and Archaeology::NH History - Abstract
In the last hundred years, the progress of science in its various disciplines has been prodigious and the University of Florence, in connection with international research groups, has developed excellent research. The progress achieved has favored the creation of new university courses and technological transfer with important advantages for the city and its economy. Examples of these interactions are physics and astronomy studies with the application development of new technologies, naturalistic studies with precious natural history collections, structural and biochemical studies for the development of new drugs and vaccines, the numerous applications of mathematical sciences, statistics and information technology and the development of science for the conservation of cultural heritage.
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- 2024
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26. Long-Term Conservation for the Safeguard of Abies nebrodensis : An Endemic and Endangered Species of Sicily.
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Benelli, Carla, Tarraf, Waed, İzgü, Tolga, Anichini, Monica, Faraloni, Cecilia, Salvatici, Maria Cristina, Jouini, Nourhene, Germanà, Maria Antonietta, Danti, Roberto, and Lambardi, Maurizio
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ENDANGERED species ,ENDEMIC species ,FIR ,TETRAZOLIUM chloride ,GERMPLASM conservation ,GERMPLASM - Abstract
The combined approaches between ex situ and in situ conservation are of great importance for threatened species in urgent need of protection. This study aims to develop concrete actions to preserve the relic of 30 adult trees of the Sicilian fir (Abies nebrodensis) from extinction using long-term germplasm conservation in liquid nitrogen (LN, −196 °C). Pollen grains were collected, and their moisture content (MC) was measured. Then, viability (2,3,5-tryphenyl tetrazolium chloride, TTC), in vitro germinability, and enzymatic antioxidant activity (ascorbate peroxidase, APX; catalase, CAT) were evaluated before and after cryopreservation. Seeds collected from mature cones underwent X-ray analysis, and only full seeds were used to excise the zygotic embryos (ZEs) for cryopreservation. The MC percentage of ZEs was determined, and then they were plunged in LN with (+PVS2) or without (−PVS2) Plant Vitrification Solution 2; untreated ZEs were used as a control. Viability (TTC test) and in vitro germination were assessed for all ZEs (+PVS2, −PVS2, and control). Embryogenic callus (EC) lines obtained from mature ZEs were cryopreserved applying the 'encapsulation-dehydration' technique. This study has allowed, after optimizing cryopreservation protocols for pollen, ZEs, and EC of A. nebrodensis, to establish the first cryobank of this endangered species in Polizzi Generosa (Palermo, Italy), inside the 'Madonie Regional Park'. The strategy developed for Sicilian fir conservation will pave the way for similar initiatives for other critically endangered conifer species. [ABSTRACT FROM AUTHOR]
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- 2024
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27. Oral or injectable semaglutide for the management of type 2 diabetes in routine care: A multicentre observational study comparing matched cohorts.
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Fadini, Gian Paolo, Bonora, Benedetta Maria, Ghiani, Mariangela, Anichini, Roberto, Melchionda, Elena, Fattor, Bruno, Fazion, Stefano, Meregalli, Giancarla, Giaccari, Andrea, Avogaro, Angelo, Consoli, Agostino, Baldassarre, Maria Antonia Pompea, Formoso, Gloria, Leto, Gaetano, Leonetti, Frida, Zavattaro, Marco, Aimaretti, Gianluca, Barale, Cristina, Cau, Rosella, and Muscarà, Andrea
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TYPE 2 diabetes ,SEMAGLUTIDE ,GLUCAGON-like peptide-1 receptor ,GLUCAGON-like peptide-1 agonists ,BODY mass index - Abstract
Aim: To investigate the real‐world utilization and comparative clinical outcomes of injectable and oral semaglutide in individuals with type 2 diabetes (T2D) with the aim of enhancing understanding of the practical implications associated with choosing between these formulations. Methods: New users of oral or injectable semaglutide were selected from a cohort of 14 079 initiators of glucagon‐like peptide‐1 receptor agonists. Propensity‐score matching (PSM) was employed to create balanced groups, ensuring comparability. The analysis encompassed dose exposure, drug persistence, and clinical outcomes, including changes in glycated haemoglobin (HbA1c) and body weight, with up to 18 months' follow‐up. Results: We analysed two matched groups of 107 participants each, who comprised on average 63.6% men, aged 64 years, with diabetes duration of approximately 10 years, body mass index of 29 kg/m2 and HbA1c level of 7.7–7.8% (61‐62 mmol/mol). The proportion of low, intermediate and high doses were similar with the oral and the injectable formulation. The change in HbA1c was similar between groups (−0.9% / ‐10 mmol/mol at 18 months) as was the proportion of individuals reaching HbA1c <6.5% (48 mmol/mol). The average change in body weight was similar in the two groups (−3.7 kg with injectable and −3.3 kg with oral at 18 months) but more new users of injectable semaglutide lost ≥5% body weight. Persistence on drug was longer with injectable than with oral semaglutide. Conclusion: In a real‐world setting, improvements in HbA1c and body weight were similar after initiation of oral or injectable semaglutide. These results may be specific to the features of the matched cohorts under investigation, with limited generalizability to populations with different characteristics. [ABSTRACT FROM AUTHOR]
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- 2024
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28. Towards real-time hyperspectral imaging in neurosurgery
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Elson, Daniel S., Gioux, Sylvain, Pogue, Brian W., Roddan, Alfie, Yu, Ziyan, Leiloglou, Maria, Chalau, Vadzim, Anichini, Giulio, Giannarou, Stamatia, and Elson, Daniel
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- 2024
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29. Multispectral Mueller matrix imaging: a potential tool to visualize white matter tracts
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Tuchin, Valery V., Blondel, Walter C. P. M., Zalevsky, Zeev, Mieites, Verónica, Anichini, Giulio, Qi, Ji, O'Neill, Kevin, Conde, Olga M., and Elson, Daniel S.
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- 2024
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30. Profiling of circulating glial cells for accurate blood‐based diagnosis of glial malignancies.
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O'Neill, Kevin, Syed, Nelofer, Crook, Timothy, Dubey, Sudhir, Potharaju, Mahadev, Limaye, Sewanti, Ranade, Anantbhushan, Anichini, Giulio, Patil, Darshana, Datta, Vineet, and Datar, Rajan
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NEUROGLIA ,BLOOD testing ,DIAGNOSIS ,BRAIN tumors ,GLIOMAS ,ASTROCYTOMAS - Abstract
Here, we describe a blood test for the detection of glial malignancies (GLI‐M) based on the identification of circulating glial cells (CGCs). The test is highly specific for GLI‐M and can detect multiple grades (II–IV) and subtypes including gliomas, astrocytomas, oligodendrogliomas, oligoastrocytomas and glioblastomas, irrespective of gender and age. Analytical validation of the test was performed as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Real‐world performance characteristics of the test were evaluated in four clinical (observational) studies. The test has high analytical sensitivity (95%), specificity (100%) and precision (coefficient of variation [CV] = 13.7% for repeatability and CV = 23.5% for within laboratory precision, both at the detection threshold) and is not prone to interference from common drugs and serum factors. The ability of the test to detect and differentiate GLI‐M from non‐malignant brain tumours (NBT), brain metastases from primary epithelial malignancies (EPI‐M) and healthy individual donors (HD) was evaluated in four clinical cohorts. Across these clinical studies, the test showed 99.35% sensitivity (95% confidence interval [CI]: 96.44%–99.98%) and 100% specificity (95% CI: 99.37%–100%). The performance characteristics of this test support its clinical utility for diagnostic triaging of individuals presenting with intracranial space‐occupying lesions (ICSOL). [ABSTRACT FROM AUTHOR]
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- 2024
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31. Epigenetic remodeling to improve the efficacy of immunotherapy in human glioblastoma: pre-clinical evidence for development of new immunotherapy approaches.
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Lofiego, Maria Fortunata, Piazzini, Francesca, Caruso, Francesca Pia, Marzani, Francesco, Solmonese, Laura, Bello, Emma, Celesti, Fabrizio, Costa, Maria Claudia, Noviello, Teresa, Mortarini, Roberta, Anichini, Andrea, Ceccarelli, Michele, Coral, Sandra, Di Giacomo, Anna Maria, Maio, Michele, and Covre, Alessia
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IMMUNE checkpoint inhibitors ,GLIOBLASTOMA multiforme ,EPIGENETICS ,TUMOR growth ,GENE expression ,BRAIN tumors - Abstract
Background: Glioblastoma multiforme (GBM) is a highly aggressive primary brain tumor, that is refractory to standard treatment and to immunotherapy with immune-checkpoint inhibitors (ICI). Noteworthy, melanoma brain metastases (MM-BM), that share the same niche as GBM, frequently respond to current ICI therapies. Epigenetic modifications regulate GBM cellular proliferation, invasion, and prognosis and may negatively regulate the cross-talk between malignant cells and immune cells in the tumor milieu, likely contributing to limit the efficacy of ICI therapy of GBM. Thus, manipulating the tumor epigenome can be considered a therapeutic opportunity in GBM. Methods: Microarray transcriptional and methylation profiles, followed by gene set enrichment and IPA analyses, were performed to study the differences in the constitutive expression profiles of GBM vs MM-BM cells, compared to the extracranial MM cells and to investigate the modulatory effects of the DNA hypomethylating agent (DHA) guadecitabine among the different tumor cells. The prognostic relevance of DHA-modulated genes was tested by Cox analysis in a TCGA GBM patients' cohort. Results: The most striking differences between GBM and MM-BM cells were found to be the enrichment of biological processes associated with tumor growth, invasion, and extravasation with the inhibition of MHC class II antigen processing/presentation in GBM cells. Treatment with guadecitabine reduced these biological differences, shaping GBM cells towards a more immunogenic phenotype. Indeed, in GBM cells, promoter hypomethylation by guadecitabine led to the up-regulation of genes mainly associated with activation, proliferation, and migration of T and B cells and with MHC class II antigen processing/presentation. Among DHA-modulated genes in GBM, 7.6% showed a significant prognostic relevance. Moreover, a large set of immune-related upstream-regulators (URs) were commonly modulated by DHA in GBM, MM-BM, and MM cells: DHA-activated URs enriched for biological processes mainly involved in the regulation of cytokines and chemokines production, inflammatory response, and in Type I/II/III IFN-mediated signaling; conversely, DHA-inhibited URs were involved in metabolic and proliferative pathways. Conclusions: Epigenetic remodeling by guadecitabine represents a promising strategy to increase the efficacy of cancer immunotherapy of GBM, supporting the rationale to develop new epigenetic-based immunotherapeutic approaches for the treatment of this still highly deadly disease. [ABSTRACT FROM AUTHOR]
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- 2024
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32. Texture analysis of 18F‐FDG PET/CT and CECT: Prediction of refractoriness of Hodgkin lymphoma with mediastinal bulk involvement.
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Abenavoli, Elisabetta M., Linguanti, Flavia, Anichini, Matilde, Miele, Vittorio, Mungai, Francesco, Palazzo, Marianna, Nassi, Luca, Puccini, Benedetta, Romano, Ilaria, Sordi, Benedetta, Sciagrà, Roberto, Simontacchi, Gabriele, Vannucchi, Alessandro M., and Berti, Valentina
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TEXTURE analysis (Image processing) ,HODGKIN'S disease ,POSITRON emission tomography ,RECEIVER operating characteristic curves ,FLUORODEOXYGLUCOSE F18 - Abstract
To recognize patients at high risk of refractory disease, the identification of novel prognostic parameters improving stratification of newly diagnosed Hodgkin Lymphoma (HL) is still needed. This study investigates the potential value of metabolic and texture features, extracted from baseline 18F‐FDG Positron Emission Tomography/Computed Tomography (PET) and Contrast‐Enhanced Computed Tomography scan (CECT), together with clinical data, in predicting first‐line therapy refractoriness (R) of classical HL (cHL) with mediastinal bulk involvement. We reviewed 69 cHL patients who underwent staging PET and CECT. Lesion segmentation and texture parameter extraction were performed using the freeware software LIFEx 6.3. The prognostic significance of clinical and imaging features was evaluated in relation to the development of refractory disease. Receiver operating characteristic curve, Cox proportional hazard regression and Kaplan‐Meier analyses were performed to examine the potential independent predictors and to evaluate their prognostic value. Among clinical characteristics, only stage according to the German Hodgkin Group (GHSG) classification system significantly differed between R and not‐R. Among CECT variables, only parameters derived from second order matrices (gray‐level co‐occurrence matrix (GLCM) and gray‐level run length matrix (GLRLM) demonstrated significant prognostic power. Among PET variables, SUVmean, several variables derived from first (histograms, shape), and second order analyses (GLCM, GLRLM, NGLDM) exhibited significant predictive power. Such variables obtained accuracies greater than 70% at receiver operating characteristic analysis and their PFS curves resulted statistically significant in predicting refractoriness. At multivariate analysis, only HISTO_EntropyPET extracted from PET (HISTO_EntropyPET) and GHSG stage resulted as significant independent predictors. Their combination identified 4 patient groups with significantly different PFS curves, with worst prognosis in patients with higher HISTO_EntropyPET values, regardless of the stage. Imaging radiomics may provide a reference for prognostic evaluation of patients with mediastinal bulky cHL. The best prognostic value in the prediction of R versus not‐R disease was reached by combining HISTO_EntropyPET with GHSG stage. [ABSTRACT FROM AUTHOR]
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- 2024
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33. Peripheral biomarker analysis in patients with advanced urothelial carcinoma (UC) after platinum chemotherapy treated with cabozantinib (CABO) plus durvalumab (DURVA): Preliminary analysis from the phase 2 ARCADIA trial.
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Giannatempo, Patrizia, Sgambelluri, Francesco, Stellato, Marco, Guadalupi, Valentina, Raggi, Daniele, Rametta, Alessandro, Bottiglieri, Achille, Claps, Melanie, Walter, Ferrari Bravo, Oldani, Simone, Zimatore, Matteo, Calareso, Giuseppina, Alessi, Alessandra, Cattaneo, Laura, Verzoni, Elena, De Braud, Filippo G., Procopio, Giuseppe, Necchi, Andrea, Anichini, Andrea, and Mortarini, Roberta
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- 2024
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34. 1806: Transcriptome and tumor immune infiltrate in breast cancer treated with preoperative radiosurgery.
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Mangoni, Monica, Duatti, Assia, Caini, Saverio, Fiorito, Giovanni, Salvatore, Giulia, Anichini, Giulia, Sottili, Mariangela, Visani, Luca, Francolini, Giulio, Di Cataldo, Vanessa, Loi, Mauro, Becherini, Carlotta, Salvestrini, Viola, Bianchi, Simonetta, Masi, Laura, Doro, Raffaella, Nori, Jacopo, Orzalesi, Lorenzo, Bernini, Marco, and Greto, Daniela
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CANCER invasiveness , *BREAST cancer , *RADIOSURGERY , *TRANSCRIPTOMES , *TUMORS - Published
- 2024
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35. Preoperative Radiosurgery on Early Breast Cancer Patients: Analysis of Tumor Genetic Background and Tumor Immune Microenvironment.
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Loi, M., Mangoni, M., Duatti, A., Caini, S., Fiorito, G., Salvatore, G., Anichini, G., Sottili, M., Visani, L., Francolini, G., Cataldo, V. Di, Becherini, C., Salvestrini, V., Bianchi, S., Stefano, G. Di, Greto, D., Simontacchi, G., Desideri, I., Meattini, I., and Livi, L.
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EPIDERMAL growth factor receptors , *GENE expression , *TUMOR classification , *SURGICAL robots , *FALSE discovery rate - Abstract
A phase II trial was conducted to assess the feasibility, safety, and activity of a single 21 Gy preoperative robotic radiosurgery (prRS) in early breast cancer patients, 50+ years old, hormonal receptors positive/human epidermal growth factor receptor 2 negative (HR+/HER2-), sized up to 25 mm. Breast surgery was performed 2 to 4 weeks after radiosurgery. A translational study was carried out to explore the modulation of tumor-immune microenvironment before and after irradiation and the transcriptome on tumor samples before irradiation. Among the 70 patients recruited, 22 patients were successfully treated with prRS. Fresh pre-treatment tumor biopsies and fresh post-radiosurgery and post-operative tumor samples were retrieved. For immunohistochemistry analysis, pre-radiosurgery biopsies and post-operative tumors were stained with specific antibodies to evaluate the expression of different immune-related biomarkers. For the transcriptome analysis, total RNA was extracted from frozen pre-treatment samples and RNA sequencing was performed by using a total of 10 ng/uL. The statistical analyses aiming at detecting differences in gene expression according to tumor downstaging were conducted by fitting age-adjusted linear regression models, and applying the false discovery rate (FDR) method for correcting for multiple tests. As clinical output, we considered tumor downstaging according to pTNM and patients were classified as Radio-Responders (R-R = 8) and non-Radio-Responders (nR-R = 13) after prRS. IHC was performed on available 19 pairs of pre- and post-irradiation tissues for evaluation of CD68, CD163, CD4, CD8, CD56 and CD16: preliminary analysis showed a marked increase in CD68, CD163 and CD4 staining after tumor prRS. RNAseq results showed 170 differentially expressed genes between R-R and nR-R. Statistical analysis identify CCDC97 and MDIFC as genes that were significantly up- and down-regulated respectively in R-R patients. CCD97 is an orphan gene previously described as part of a nuclear interactome with functional role in DNA damage repair, while MDFIC is reported to be associated with pro- or anti-tumoral functions as chemoresistance induction or cell proliferation inhibition. The phase II trial enrolled 22 patients; this little number limits the statistical power of results obtained in the translational analysis. Based on the preliminary results, IHC analysis showed that prRS modulates the immune microenvironment composition in early breast cancer patients. Furthermore, we hypothesized that the differential expression of CCDC97 and MDFIC may play a role in prRS response. The role of orphan genes CCDC97 and MDFIC in response to radiation and to hypofractionation makes worthy further pre- and clinical investigations. [ABSTRACT FROM AUTHOR]
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- 2024
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36. LBA47 Nivolumab plus ipilimumab and ASTX727 or nivolumab plus ipilimumab in PD-1 resistant metastatic melanoma: Primary analysis of the randomized phase II NIBIT-ML1 study.
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Di Giacomo, A.M., Simonetti, E., Calabro, L., Vegni, V., Santangelo, F., Depenni, R., Colucci, M., Valente, M., Grifoni, R., Lofiego, M.F., Amato, G., Keer, H.N., Oganesian, A., Chan, D., Giannarelli, D., Altomonte, M., Ceccarelli, M., Anichini, A., Covre, A., and Maio, M.
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NIVOLUMAB , *PROGRAMMED cell death 1 receptors , *IPILIMUMAB , *MELANOMA , *METASTASIS - Published
- 2024
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37. 1916P Methylation subtypes correlate with tumor immune contextures and outcome to ICI therapy of pleural mesothelioma (PM) patients: The NIBIT EPI-MESO study.
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Calabro, L., Caruso, F.P., Covre, A., Lofiego, M.F., Noviello, T., Tufano, R., Lagano, V., Nastasi, N., Sabella, G., Rossi, G., Coral, S., Grosso, F., Cerbone, L., Delfanti, S., Di Giacomo, A.M., Milione, M., Mortarini, R., Anichini, A., Ceccarelli, M., and Maio, M.
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MESOTHELIOMA , *METHYLATION , *TUMORS - Published
- 2024
- Full Text
- View/download PDF
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