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Introduction: Point-of-care (POC) manufacturing of chimeric antigen receptor (CAR) modified T cell has expanded rapidly over the last decade. In addition to the use of CD19 CAR T cells for hematological diseases, there is a growing interest in targeting a variety of tumor-associated epitopes. Methods: Here, we report the manufacturing and characterization of autologous anti-CD20 CAR T cells from melanoma patients within phase I clinical trial (NCT03893019). Using a second-generation lentiviral vector for the production of the CD20 CAR T cells on the CliniMACS Prodigy®. Results: We demonstrated consistency in cell composition and functionality of the products manufactured at two different production sites. The T cell purity was >98.5%, a CD4/CD8 ratio between 2.5 and 5.5 and transduction rate between 34% and 61% on day 12 (harvest). Median expansion rate was 53-fold (range, 42-65-fold) with 1.7-3.8×109 CAR T cells at harvest, a sufficient number for the planned dose escalation steps (1×105/kg, 1×106/kg, 1×107/kg BW). Complementary research of some of the products pointed out that the CAR+ cells expressed mainly central memory T-cell phenotype. All tested CAR T cell products were capable to translate into T cell activation upon engagement of CAR target cells, indicated by the increase in pro-inflammatory cytokine release and by the increase in CAR T cell amplification. Notably, there were some interindividual, cell-intrinsic differences at the level of cytokine release and amplification. CAR-mediated T cell activation depended on the level of CAR cognate antigen. Discussion: In conclusion, the CliniMACS Prodigy® platform is well suited for decentralized POC manufacturing of anti-CD20 CAR T cells and may be likewise applicable for the rapid and automated manufacturing of CAR T cells directed against other targets. [ABSTRACT FROM AUTHOR]

After World War II, radical right parties in Europe experienced a decline, but over the last three decades, they have regained strength, becoming significant players in European politics. Since the early twenty-first century, they have secured representation at various levels, from local governments to national parliaments and the European Parliament. Nowadays, populist radical right parties rank among the top three most powerful political forces in one-third of European countries. In Austria, the FPÖ (Freiheitliche Partei Österreichs) emerged as the leading party in the 2024 elections, winning 28.9% of the vote. In Italy, the Fratelli d’Italia coalition came to power with 25.99% of the vote in 2022. In Hungary, Viktor Orbán’s Fidesz party won its fourth consecutive election. In France, Marine Le Pen’s National Rally (RN – Rassemblement National) achieved major victories in both the European Parliament and national elections, becoming the country’s largest party. Though initially considered marginal, populist radical right parties have become a lasting and serious force in European politics. This special issue features six carefully selected articles, each offering empirical and theoretical insights into different aspects of the rise of radical right and right-wing populist parties in Europe, contributing to a broader understanding of this phenomenon. [ABSTRACT FROM AUTHOR]