The wattle tree (Acacia mearnsii) is gaining importance as an exotic species in northwestern Ethiopia, providing ecological, environmental, and economic benefits, especially for fuelwood and charcoal production. This study aimed to investigate the effect of tree spacing on the growth and biomass of wattle trees. The study employed a randomized complete block design with three replications across three spacing treatments (0.5 m × 0.5 m, 1 m × 1 m, and 1.5 m × 1.5 m) in the Fagita district. Tree height and diameter measurements were taken at 12, 18, and 30 months post-planting from stands established in 2021. The results showed significant differences in tree height among the spacing treatments at 18 and 30 months. The closest spacing (0.5 m × 0.5 m) yielded the highest average tree heights of 32.12 cm, 84.86 cm, and 302.98 cm at 12, 18, and 30 months, respectively. At 18 months, the largest average diameter (1.22 cm) was found in the narrowest spacing (0.5 m × 0.5 m), whereas at 30 months, the widest spacing (1.5 m × 1.5 m) recorded the largest diameter (1.51 cm). Throughout the study, height, diameter, and average aboveground biomass exhibited an inverse relationship with spacing, with this effect diminishing as trees aged. Tree spacing significantly impacted average aboveground biomass at 18 months, with the densest spacing (0.5 m × 0.5 m) yielding the highest average aboveground biomass (1.97 kg at 18 months and 2.41 kg at 30 months). Average aboveground biomass increased as the trees matured. These findings suggest that closer spacing of A. mearnsii can enhance biomass production, positioning it as a promising candidate for energy generation. Leveraging these insights can optimize resource utilization while supporting global energy demands and reforestation initiatives aimed at carbon sequestration. [ABSTRACT FROM AUTHOR]