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Single-dose azithromycin is being considered by the WHO as an intervention for prevention of child mortality. However, concerns have emerged related to longer term unintended consequences of early life antibiotic use, particularly among infants. We conducted a long-term follow-up in a random sample of children who had been enrolled in a trial of neonatal azithromycin versus placebo for prevention of mortality to assess whether neonatal azithromycin exposure led to differences in child growth up to 4 years of age. We found no evidence of a difference in any anthropometric outcome among children who had received a single oral dose of azithromycin compared with placebo during the neonatal period. These results do not support long-term growth-promoting or deleterious effects of early life azithromycin exposure.

ImportanceBefore surgery, the best strategy for managing patients who are taking renin-angiotensin system inhibitors (RASIs) (angiotensin-converting enzyme inhibitors or angiotensin receptor blockers) is unknown. The lack of evidence leads to conflicting guidelines.ObjectiveTo evaluate whether a continuation strategy vs a discontinuation strategy of RASIs before major noncardiac surgery results in decreased complications at 28 days after surgery.Design, setting, and participantsRandomized clinical trial that included patients who were being treated with a RASI for at least 3 months and were scheduled to undergo a major noncardiac surgery between January 2018 and April 2023 at 40 hospitals in France.InterventionPatients were randomized to continue use of RASIs (n = 1107) until the day of surgery or to discontinue use of RASIs 48 hours prior to surgery (ie, they would take the last dose 3 days before surgery) (n = 1115).Main outcomes and measuresThe primary outcome was a composite of all-cause mortality and major postoperative complications within 28 days after surgery. The key secondary outcomes were episodes of hypotension during surgery, acute kidney injury, postoperative organ failure, and length of stay in the hospital and intensive care unit during the 28 days after surgery.ResultsOf the 2222 patients (mean age, 67 years [SD, 10 years]; 65% were male), 46% were being treated with angiotensin-converting enzyme inhibitors at baseline and 54% were being treated with angiotensin receptor blockers. The rate of all-cause mortality and major postoperative complications was 22% (245 of 1115 patients) in the RASI discontinuation group and 22% (247 of 1107 patients) in the RASI continuation group (risk ratio, 1.02 [95% CI, 0.87-1.19]; P = .85). Episodes of hypotension during surgery occurred in 41% of the patients in the RASI discontinuation group and in 54% of the patients in the RASI continuation group (risk ratio, 1.31 [95% CI, 1.19-1.44]). There were no other differences in the trial outcomes.Conclusions and relevanceAmong patients who underwent major noncardiac surgery, a continuation strategy of RASIs before surgery was not associated with a higher rate of postoperative complications than a discontinuation strategy.Trial registrationClinicalTrials.gov Identifier: NCT03374449.

ImportanceRepeated mass distribution of azithromycin has been shown to reduce childhood mortality by 14% in sub-Saharan Africa. However, the estimated effect varied by location, suggesting that the intervention may not be effective in different geographical areas, time periods, or conditions.ObjectiveTo evaluate the efficacy of twice-yearly azithromycin to reduce mortality in children in the presence of seasonal malaria chemoprevention.Design, setting, and participantsThis cluster randomized placebo-controlled trial evaluating the efficacy of single-dose azithromycin for prevention of all-cause childhood mortality included 341 communities in the Nouna district in rural northwestern Burkina Faso. Participants were children aged 1 to 59 months living in the study communities.InterventionsCommunities were randomized in a 1:1 ratio to receive oral azithromycin or placebo distribution. Children aged 1 to 59 months were offered single-dose treatment twice yearly for 3 years (6 distributions) from August 2019 to February 2023.Main outcomes and measuresThe primary outcome was all-cause childhood mortality, measured during a twice-yearly enumerative census.ResultsA total of 34 399 children (mean [SD] age, 25.2 [18] months) in the azithromycin group and 33 847 children (mean [SD] age, 25.6 [18] months) in the placebo group were included. A mean (SD) of 90.1% (16.0%) of the censused children received the scheduled study drug in the azithromycin group and 89.8% (17.1%) received the scheduled study drug in the placebo group. In the azithromycin group, 498 deaths were recorded over 60 592 person-years (8.2 deaths/1000 person-years). In the placebo group, 588 deaths were recorded over 58 547 person-years (10.0 deaths/1000 person-years). The incidence rate ratio for mortality was 0.82 (95% CI, 0.67-1.02; P = .07) in the azithromycin group compared with the placebo group. The incidence rate ratio was 0.99 (95% CI, 0.72-1.36) in those aged 1 to 11 months, 0.92 (95% CI, 0.67-1.27) in those aged 12 to 23 months, and 0.73 (95% CI, 0.57-0.94) in those aged 24 to 59 months.Conclusions and relevanceMortality in children (aged 1-59 months) was lower with biannual mass azithromycin distribution in a setting in which seasonal malaria chemoprevention was also being distributed, but the difference was not statistically significant. The study may have been underpowered to detect a clinically relevant difference.Trial registrationClinicalTrials.gov Identifier: NCT03676764.

Mass antibiotic distribution to preschool children resulted in alterations of the gut microbiome months after distribution. This individually randomized, placebo-controlled trial evaluated changes in the gut microbiome and resistome in children aged 8 days to 59 months after one dose of oral azithromycin in Burkina Faso. A total of 450 children were randomized in a 1:1 ratio to either placebo or azithromycin. Rectal samples were collected at baseline, 2 weeks, and 6 months after randomization and subjected to DNA deep sequencing. Gut microbiome diversity and normalized antimicrobial resistance determinants for different antibiotic classes were evaluated. Azithromycin decreased gut bacterial diversity (Shannon P < 0.0001; inverse Simpson P < 0.001) 2 weeks after treatment relative to placebo. Concurrently, the normalized abundance of macrolide resistance genetic determinants was 243-fold higher (95% CI: 76-fold to 776-fold, P < 0.0001). These alterations did not persist at 6 months, suggesting that disruptions were transient. Furthermore, we were unable to detect resistance changes in other antibiotic classes, indicating that co-resistance with a single course of azithromycin when treated at the individual level was unlikely.

The sense of agency (SoA) refers to the experience of controlling one's actions and their effects, while representational momentum (RM) denotes a bias in the perceived trajectory of a moving object induced by one's anticipation of movement. Research in cognitive science suggests that control over action modulates anticipative mechanisms. In the present study, we question the influence of SoA on RM. Participants viewed two dots, one of which moved horizontally on the screen. Its movement was either triggered by the computer or by participants. In the former case, participants either could freely choose or were commanded on which dot to trigger. Additionally, given the role of affordances in motor control and movement perception, we tested the effect of adding a tunnel through which the dot could pass. The results showed that agency and affordances influenced movement anticipation with no interaction between the two. Freedom of choice yielded no difference.

BACKGROUND: Antibiotic use during early infancy has been linked to childhood obesity in high-income countries. We evaluated whether a single oral dose of azithromycin administered during infant-well visits led to changes in infant growth outcomes at 6 months of age in a setting with a high prevalence of undernutrition in rural Burkina Faso. METHODS AND FINDINGS: Infants were enrolled from September 25, 2019, until October 22, 2022, in a randomized controlled trial designed to evaluate the efficacy of a single oral dose of azithromycin (20 mg/kg) compared to placebo when administered during well-child visits for prevention of infant mortality. The trial found no evidence of a difference in the primary endpoint. This paper presents prespecified secondary anthropometric endpoints including weight gain (g/day), height change (mm/day), weight-for-age Z-score (WAZ), weight-for-length Z-score (WLZ), length-for-age Z-score (LAZ), and mid-upper arm circumference (MUAC). Infants were eligible for the trial if they were between 5 and 12 weeks of age, able to orally feed, and their families were planning to remain in the study area for the duration of the study. Anthropometric measurements were collected at enrollment (5 to 12 weeks of age) and 6 months of age. Among 32,877 infants enrolled in the trial, 27,298 (83%) were followed and had valid anthropometric measurements at 6 months of age. We found no evidence of a difference in weight gain (mean difference 0.03 g/day, 95% confidence interval (CI) -0.12 to 0.18), height change (mean difference 0.004 mm/day, 95% CI -0.05 to 0.06), WAZ (mean difference -0.004 SD, 95% CI -0.03 to 0.02), WLZ (mean difference 0.001 SD, 95% CI -0.03 to 0.03), LAZ (mean difference -0.005 SD, 95% CI -0.03 to 0.02), or MUAC (mean difference 0.01 cm, 95% CI -0.01 to 0.04). The primary limitation of the trial was that measurements were only collected at enrollment and 6 months of age, precluding assessment of shorter-term or long-term changes in growth. CONCLUSIONS: Single-dose azithromycin does not appear to affect weight and height outcomes when administered during early infancy. TRIAL REGISTRATION: ClinicalTrials.gov NCT03676764.

Abstract Background Streptococcus pneumoniae (S. pneumoniae), Haemophilus influenzae (H. influenzae), and Neisseria meningitidis (N. meningitidis) are leading causes of childhood bacterial meningitis and preventable by vaccines. The aim of this hospital-based sentinel surveillance is to describe the epidemiological characteristics of pneumococcal meningitis, including disease burden, and to provide baseline data on pneumococcal serotype distribution to support decision making for pneumococcal conjugate vaccine (PCV) introduction in Vietnam. Methods Surveillance for probable bacterial meningitis in children 1–59 months of age is conducted in three tertiary level pediatric hospitals: one in Hanoi and two in Ho Chi Minh City. Cerebrospinal fluid (CSF) specimens were collected via lumbar puncture from children with suspected meningitis. Specimens were transferred immediately to the laboratory department of the respective hospital for cytology, biochemistry, and microbiology testing, including culture. PCR testing was conducted on CSF specimens for bacterial detection (S. pneumoniae, H. influenzae, and N. meningitidis) and pneumococcal serotyping. Results During 2015–2018, a total of 1,803 children with probable bacterial meningitis were detected; 1,780 had CSF specimens available for testing. Of 245 laboratory-confirmed positive cases, the majority were caused by S. pneumoniae (229,93.5%). Of those with S. pneumoniae detected, over 70% were caused by serotypes included in currently available PCV products; serotypes 6 A/6B (27.1%), 14 (19.7%), and 23 F (16.2%) were the most common serotypes. Children with laboratory-confirmed pneumococcal meningitis were more likely to live in Hanoi (p

Abstract Background Schistosomiasis remains a public health problem, particularly in sub-Saharan Africa. The disease is intimately connected to poverty and environmental factors. Our research was readily embedded into a multi-country schistosomiasis oversampling study. The aim of the study presented here was to determine the prevalence of Schistosoma mansoni and to investigate the role of water body characteristics and water-related human activities in disease transmission. Methods In August and September 2022, a cross-sectional study was conducted in the western part of Côte d’Ivoire. Stool and urine samples were collected from 1602 and 1729 children aged 5–14 years, respectively, in 65 villages in the health districts of Biankouma, Ouaninou and Touba. Additionally, data were collected from direct observation of water-related activities at water bodies and interviews conducted with community leaders and health workers. The prevalence and risk factors for Schistosoma infection were assessed using generalised estimating equation models. Results The prevalence ofS. mansoni and S. haematobium were 27.4% (95% confidence interval [CI] 21.5–34.3%) and 0.1% (95% CI 0.03–0.5%), respectively. Low prevalence of soil-transmitted helminths was observed with 2.4%, 0.4% and 0.2% for hookworm, Trichuris trichiura and Ascaris lumbricoides, respectively. At the health district level, we found S. mansoni prevalence of 34.4% (95% CI 25.0–45.3%), 34.3% (95% CI 24.0–46.2%) and 16.3% (95% CI 9.5–26.6%) for Biankouma, Ouaninou and Touba, respectively. Female and male participants were at a similar risk of infection (29.0% vs. 26.0%, odds ratio [OR]: 1.18, 95% CI 0.92–1.50). Children aged 9–14 years showed a higher prevalence than their younger counterparts aged 5–8 years (34.5% vs. 22.7%, OR: 1.80, 95% CI 1.42–2.27). High infection prevalence was observed in villages where children were washing clothes and dishes at open surface water sites and pursued recreational activities (e.g. swimming and playing in the water). The temperature, total dissolved solids and pH of water samples showed no significant association with S. mansoni infection at the village unit. Conclusions Human water-related activities such as washing clothes and playing in the water are risk factors for S. mansoni transmission. Hence, preventive chemotherapy should be combined with information, education and communication to avoid or reduce the frequency of water exposure in children as part of a comprehensive package of interventions towards elimination of schistosomiasis as a public health problem. Graphical Abstract

Abstract Background In addition to antibiotic resistance, persistence is another cause of treatment failure in bacterial infections, representing a significant public health concern. Due to a lack of adequate data on clinical isolates, this study was initiated to investigate persistence in clinical isolates in Burkina Faso. Methods Eighty (80) clinical isolates, including 32 Pseudomonas aeruginosa, 41 Staphylococcus aureus, and 7 Salmonella sp. obtained from clinical laboratories in Burkina Faso, were analyzed to assess their susceptibility to ciprofloxacin and gentamicin, as well as to determine the presence of persistence genes. The effects of ciprofloxacin and gentamicin on persister formation were evaluated by conducting colony counts at 1, 3, 5, 7, and 20 h after exposing the bacteria to high concentrations of these antibiotics. Results Results showed high sensitivity to both antibiotics (72.5% for ciprofloxacin and 82.5% for gentamicin). Persister formation occurred in Staphylococcus aureus with gentamicin and in Salmonella sp. with ciprofloxacin, while Pseudomonas aeruginosa did not form persisters. The mazF gene was found in 28.13% of P. aeruginosa and 2.44% of S. aureus isolates, and the hipA gene in 28.57% of Salmonella sp. None of the relE1 or relE2 genes were detected. Conclusions The study revealed high sensitivity in clinical bacterial isolates to ciprofloxacin and gentamicin. Staphylococcus aureus and Salmonella sp. showed persister formation under antibiotic stress, with low frequencies of the studied persistence genes. These findings enhance understanding of clinical bacterial behavior and inform strategies against antibiotic-resistant infections.

Environmental DNA (eDNA) includes a set of rapidly emerging technologies that have the potential to support environmental monitoring and biodiversity conservation through novel, non-invasive, cost-effective and democratic methods and tools. Meanwhile, eDNA researchers are developing international standards for eDNA technologies, methods and data outputs. For eDNA technologies to be accessible, useful and appropriate, we must ensure that any standards developed include a broad conception of users from around the world, a diversity of ecological contexts and locations and, most importantly, a realistic outlook on research capacities and infrastructure. In this article, we assemble perspectives on international standardisation of eDNA from a diverse and global group of users and experts from Africa, South America and the Pacific Islands. The authors of this article collaborated by answering and discussing a set of open-ended questions aimed at eliciting hopes, concerns and experiences regarding eDNA standards. The result is a set of emergent themes and a generative consensus to highlight the need for the creation of adaptable standards, the development of regional capacity, increased sensitising to data sovereignty and the viewing of standardisation as a global capacity-building activity.

Abstract Palm trees are multi-purpose species that are involved in several socio-economic and cultural aspects of Niger people. Indeed, palm trees significantly improve local population life. This study aims to assess the impact of future climate change on the geographical distribution of Borassus aethiopum Mart., Hyphaene thebaica (L.) Mart. and Phoenix dactylifera L. and to determine the suitable habitats for the conservation and sustainable management of these species in Niger. The maxEnt approach was used to model the species distribution. Four climatic models of CHELSA V2.1 were used: gfdl-esm4, ipsl-cm6a-lr, mpi-esm1-2h, mri-esm2-0 under two scenarios: ssp1-2.6 and ssp5-8.5 by periods 2011–2040 and 2041–2070. The mean AUC was 0.93 for Borassus aethiopum (0.93), 0.86 for Hyphaene thebaica, and 0.87 for Phoenix dactylifera. TSS test mean were 0.85, 0.69, and 0.75 respectively for Borassus aethiopum, Hyphaene thebaica, and Phoenix dactylifera. The current distribution for highly suitable habitats was 33,223 km2 (2.22%), 159,495 km2 (10.66%), and 108,189 km2 (7%) respectively for Borassus aethiopum, Hyphaene thebaica, and Phoenix dactylifera. The moderately suitable habitats were 83,019 km2 (5.55%) for Borassus aethiopum, 86,239 km2 (5.76%) for Hyphaene thebaica and 66,426 km2 (4%) Phoenix dactylifera. The lowly suitable habitats were 137,859 km2 (9.21%), 201,609 km2 (13.48%), and 333,181 km2 (22%) respectively Borassus aethiopum, Hyphaene thebaica, and Phoenix dactylifera. The highly and moderately suitable habitats for Borassus aethiopum will increase over the period 2011–2040 and 2041–2070. The highly suitable habitats of Hyphaene thebaica will be highly decreased by the period 2011–2040 and 2041–2070 than the other suitable habitats. All suitable habitats and all models of Phoenix dactylifera will decrease under the period 2041–2070. Phoenix dactylifera and Hyphaene thebaica will be much more affected by future climate change. This will also affect production rates for farmers in Niger.

Abstract Background Campylobacter spp. is a significant etiological agent of bacterial gastroenteritis globally. In Burkina Faso (BFA), the actual impact of this pathogen on gastroenteritis is considerably underestimated, primarily due to inadequate surveillance systems. Objectives This study aimed to investigate the proportion of Campylobacter species responsible for acute gastroenteritis among patients of all ages in urban and rural areas of BFA, using molecular biology techniques. Study design & methods Between 2018 and 2021, faecal specimens were obtained from 1,295 individuals presenting with acute gastroenteritis. These samples underwent screening for the Campylobacter coli/jejuni/lari complex utilizing real-time polymerase chain reaction (PCR) assays. Subsequently, positive samples were subjected to species-level differentiation through the application of species-specific primers. Results Campylobacter spp. was detected in 25.0% (324/1,295) of the samples analysed. The majority of positive samples (95%, 308/324) were obtained from children under 5 years of age. Species identification was performed on a subset of 114 isolates, revealing 51 Campylobacter jejuni, 10 Campylobacter coli, and 53 Campylobacter isolates that remained unspeciated. Conclusions This study reveals a significant prevalence of Campylobacter species among patients with acute gastroenteritis, with a particularly high incidence observed in children under 5 years of age. Based on these findings, the implementation of routine Campylobacter surveillance in public health laboratories is strongly recommended to better monitor and address this health concern.

Curricular reforms oftentimes cause more problems than they solve. Teachers' being kept out of curricular activities is one main reason for a reform to plummet. In this qualitative study, we examined the Malian curricular policy from two aspects -- reform and teaching and learning. Guided by Fischer's argumentative approach theory, this analysis involved 18 participants. While officials and principals were interviewed, teachers were both interviewed and observed. We tried to elaborate on the power of arguments to further principals' and teachers' concerns and needs in curriculum related tasks. We discovered that CBA reform has the potential to harness learner's chances to learn better. The identified challenges like the intricate dialogue between authorities and teachers need to be lifted to unlock the full potentiality of the reform and achieve goals. We also found that advocating for teachers' inclusion in curriculum-making remains as important as upskilling teachers' competence to contribute to curriculum processes. (Re-)Designing standardized textbooks can address the issues of curriculum content overload and concept opacity. This can also help unify practices and align content with contextual norms. To curb congestion, a typical phenomenon in resources-scarce areas like Mali, more classes or schools should mushroom.

Burkina Faso is a landlocked country within the meningitis belt of sub-Saharan Africa that experiences hyperendemic bacterial meningitis and an elevated risk for recurrent meningitis outbreaks (1). Commonly characterized by [...]

Background: Environmental mycobacteria are involved in several infections ranging from lung to skin infections. In Côte d’Ivoire, apart from Mycobacterium ulcerans and Mycobacterium tuberculosis, little information exists on other species. The culture of these species, a real challenge, especially in developing countries like Cote d’Ivoire, limits their identification. However, there are reports in literature of infections caused by these mycobacteria, and few species have never been described in human or animal infections. These are difficult cases to treat because of their resistance to most antituberculosis antibiotics. The aim of our work was to study the diversity of potentially pathogenic mycobacterial species in wastewater drainage channels in different townships and in two hospital effluents in the city of Abidjan. Methods: Wastewater samples were cultured, followed by conventional polymerase chain reaction (PCR) targeting mycobacterial 16S ribonucleic acid (16S RNA) using PA/MSHA primers. 16 S RNA identified were sequenced by Sanger techniques. Sequences obtained were analyzed, and a phylogenic tree was built. Results: Fast-growing mycobacteria, including Mycobacterium fortuitum, Mycobacterium phocaicum, Mycobacterium sp., and others presence, were confirmed both by culture and molecular techniques. M. fortuitum strain was the same in effluents of the Treichville University Hospital and in the wastewater of the township of Koumassi. New species never isolated in Côte d’Ivoire, such as M. phocaicum, have been identified in wastewater of the township of Yopougon. Conclusion: This study showed that the sewer network in the city of Abidjan is colonized by both potentially pathogenic mycobacteria and saprophytic environmental mycobacteria.

This paper analyzes the effects of regional economic determinants on stock market volatility especially for the BRVM Stock Exchange by using Generalized Autoregressive Conditional Heteroscedasticity (GARCH) models with the data from March 2005 to September 2023. The effects of economic determinants as the USD exchange rate, CNY exchange rate, inflation rate, and average interest rate on credits on stock market volatility is tested with GARCH (1,1) models. The results show complex relationships, including an inverse relationship between stock market volatility, the USD rate, the CNY rate and AIR rate. However, inflation rate has a significant impact on stock market volatility. In the dynamic landscape of the BRVM Stock Exchange, the study offers useful insights for risk management, policy considerations, and investment decisions enabling market participants to make appropriate decisions. Moreover, the study's outcomes bear relevance to policymakers, offering guidance for shaping economic policies that may influence stock market dynamics. Additionally, investors may benefit from the research by getting a broad view of the determinants that may affect stock prices, thereby enhancing their ability to formulate sound investment strategies.

Abstract Background The recent reduction in malaria burden in Côte d’Ivoire is largely attributable to the use of long-lasting insecticidal nets (LLINs). However, this progress is threatened by insecticide resistance and behavioral changes in Anopheles gambiae sensu lato (s.l.) populations and residual malaria transmission, and complementary tools are required. Thus, this study aimed to assess the efficacy of the combined use of LLINs and Bacillus thuringiensis israelensis (Bti), in comparison with LLINs. Methods This study was conducted in the health district of Korhogo, northern Côte d'Ivoire, within two study arms (LLIN + Bti arm and LLIN-only arm) from March 2019 to February 2020. In the LLIN + Bti arm, Anopheles larval habitats were treated every fortnight with Bti in addition to the use of LLINs. Mosquito larvae and adults were sampled and identified morphologically to genus and species using standard methods. The members of the An. gambiae complex were determined using a polymerase chain reaction technique. Plasmodium infection in An. gambiae s.l. and malaria incidence in local people was also assessed. Results Overall, Anopheles spp. larval density was lower in the LLIN + Bti arm 0.61 [95% CI 0.41–0.81] larva/dip (l/dip) compared with the LLIN-only arm 3.97 [95% CI 3.56–4.38] l/dip (RR = 6.50; 95% CI 5.81–7.29; P

Abstract Noroviruses are the second leading cause of death in children under the age of 5 years old. They are responsible for 200 million cases of diarrhoea and 50,000 deaths in children through the word, mainly in low-income countries. The objective of this review was to assess how the prevalence and genetic diversity of noroviruses have been affected by the introduction of rotavirus vaccines in Africa. PubMed, Web of Science and Science Direct databases were searched for articles. All included studies were conducted in Africa in children aged 0 to 5 years old with gastroenteritis. STATA version 16.0 software was used to perform the meta-analysis. The method of Dersimonian and Laird, based on the random effects model, was used for the statistical analyses in order to estimate the pooled prevalence’s at a 95% confidence interval (CI). Heterogeneity was assessed by Cochran’s Q test using the I2 index. The funnel plot was used to assess study publication bias. A total of 521 studies were retrieved from the databases, and 19 were included in the meta-analysis. The pooled norovirus prevalence’s for pre- and post-vaccination rotavirus studies were 15% (95 CI, 15–18) and 13% (95 CI, 09–17) respectively. GII was the predominant genogroup, with prevalence of 87.64% and 91.20% respectively for the pre- and post-vaccination studies. GII.4 was the most frequently detected genotype, with rates of 66.84% and 51.24% respectively for the pre- and post-vaccination studies. This meta-analysis indicates that rotavirus vaccination has not resulted in a decrease in norovirus infections in Africa.

Abstract Background Vector control using insecticides is a key prevention strategy against malaria. Unfortunately, insecticide resistance in mosquitoes threatens all progress in malaria control. In the perspective of managing this resistance, new insecticide formulations are being tested to improve the effectiveness of vector control tools. Methods The efficacy and residual activity of Pirikool® 300 CS was evaluated in comparison with Actellic® 300 CS in experimental huts at the Tiassalé experimental station on three substrates including cement, wood and mud. The mortality, blood-feeding inhibition, exiting behaviour and deterrency of free-flying wild mosquitoes was evaluated. Cone bioassay tests with susceptible and resistant mosquito strains were conducted in the huts to determine residual efficacy. Results A total of 20,505 mosquitoes of which 10,979 (53%) wild female Anopheles gambiae were collected for 112 nights. Residual efficacy obtained from monthly cone bioassay was higher than 80% with the susceptible, laboratory-maintained An. gambiae Kisumu strain, from the first to the tenth study period on all three types of treated substrate for both Actellic® 300CS and Pirikool® 300CS. This residual efficacy on the wild Tiassalé strain was over 80% until the 4th month of study on Pirikool® 300CS S treated substrates. Overall 24-h mortalities of wild free-flying An. gambiae sensu lato which entered in the experimental huts over the 8-months trial on Pirikool® 300CS treatment was 50.5%, 75.9% and 52.7%, respectively, on cement wall, wood wall and mud wall. The positive reference product Actellic® 300CS treatment induced mortalities of 42.0%, 51.8% and 41.8% on cement wall, wood wall and mud wall. Conclusion Pirikool® 300CS has performed really well against resistant strains of An. gambiae using indoor residual spraying method in experimental huts. It could be an alternative product for indoor residual spraying in response to the vectors' resistance to insecticides.

Abstract Background The direct membrane feeding assay (DMFA), whereby gametocyte-infected blood is collected from human donors and from which mosquitoes feed through a membrane, is proving essential for assessing parameters influencing Plasmodium transmission potential in endemic countries. The success of DMFAs is closely tied to gametocyte density in the blood, with relatively high gametocytaemia ensuring optimal infection levels in mosquitoes. As transmission intensity declines with control efforts, the occurrence of asymptomatic individuals with low gametocyte densities, who can significantly contribute to the infectious reservoir, is increasing. This poses a limitation to studies relying on the experimental infection of large numbers of mosquitoes with natural isolates of Plasmodium. A simple, field-applicable method is presented for improving parasite infectivity by concentrating Plasmodium falciparum gametocytes. Methods Anopheles gambiae received one of the following 5 blood treatments through DMFA: (i) whole blood (WB) samples from naturally-infected donors; (ii) donor blood whose plasma was replaced with the same volume of Plasmodium-naive AB + serum (1:1 control); (iii) plasma replaced with a volume of malaria-naïve AB + serum equivalent to half (1:1/2), or to a quarter (1:1/4), of the initial plasma volume; and (v) donor blood whose plasma was fully removed (RBC). The experiment was repeated 4 times using 4 distinct wild parasite isolates. Seven days post-infection, a total of 1,095 midguts were examined for oocyst presence. Results Substituting plasma with reduced amounts (1:1/2 and 1:1/4) of Plasmodium-naive AB + serum led to a 31% and 17% increase of the mosquito infection rate and to a 85% and 308% increase in infection intensity compared to the 1:1 control, respectively. The full removal of plasma (RBC) reduced the infection rate by 58% and the intensity by 64% compared to the 1:1 control. Reducing serum volumes (1:1/2; 1:1/4 and RBC) had no impact on mosquito feeding rate and survival when compared to the 1:1 control. Conclusions Concentrating gametocytic blood by replacing natural plasma by lower amount of naive serum can enhance the success of mosquito infection. In an area with low gametocyte density, this simple and practical method of parasite concentration can facilitate studies on human-to-mosquito transmission such as the evaluation of transmission-blocking interventions.

Abstract Background Universal coverage with insecticide-treated nets (ITNs) is important for malaria control and elimination. The emergence and intensification of insecticide resistance threatens progress made through the deployment of these interventions and has required the development of newer, more expensive ITN types. Understanding malaria prevention behaviour, including barriers and facilitators to net access and use, can support effective decision-making for the promotion and distribution of ITNs. Methods In-depth interviews and focus group discussions were conducted in 3 to 4 villages per district, in 13 districts across Burkina Faso, Mozambique, Nigeria and Rwanda from 2019 to 2022. Interviews were conducted in the local language, translated and transcribed in English, French or Portuguese. Transcripts were coded and analysed using Nvivo and ATLAS.ti. Results ITNs were obtained from mass distribution campaigns, antenatal care and immunization visits, and purchased on the private market in some locations. While there were divergent perspectives in whether the number of distributed nets were adequate, participants consistently expressed concerns of bias, discrimination, and a lack of transparency with the distribution process. ITNs were frequently used alongside other malaria prevention methods. The primary motivation for use was malaria prevention. While some participants reported using nets nightly throughout the year, other participants reported seasonal use, both due to the perceived higher density of mosquitoes and discomfort of sleeping under a net in the increased heat. Other barriers to consistent net use included activities that take place away from the home, sleeping patterns and arrangements, and sensitivity to the insecticides on the nets. Conclusions ITNs remain an important malaria control intervention. To ensure adequate and increased net access, distribution campaigns should consider family structures, available sleeping spaces, and other bed sharing preferences when identifying the number of nets needed for distribution. In addition, campaigns should allow for multiple options for net distribution points and timing to accommodate households remote to health services. Continuous distribution channels and complimentary distribution through the private sector could help fill gaps in coverage. Solutions are needed for outdoor malaria transmission, including alternative designs for ITNs, and improving access to complementary personal protective measures.

Background: From birth to 6 months, children must be exclusively breastfed, and from 6 months, food diversification starts to supplement breast milk. This cross-sectional study aims to evaluate some of the feeding practices regarding children aged 6 to 23 months in the city of Ouagadougou. Methods: First, data were food consumption data as well as recalling meals over the past 24 hours were collected. Then, technological and labeling data regarding infant flours produced locally and sold in Ouagadougou were obtained. For analysis, data were entered into Sphinx V5 and IBM SPSS Statistics 20 software, and means were compared using XLSTAT 2016 software. Results: In total, 33 local infant flours were evaluated, and all the formulations contained cereals. Fruits and legumes (30.30%), milk (30.30%), and eggs (0,00%) were the least used materials. Instant type flours were rarely encountered (9.09%), and packaging in plastic bags was consumed the most (57.57%). Regarding labeling information, batch number (28%) and the method of preservation (32%) were the least identified. With respect to food consumption, 94.84% of the children were breastfed and 98.33% aged 6 to 8 months consumed solid foods. At least 85.28% of women considered the age of 6 months for introduction of solid foods. Concerning consumption of infant porridges, imported infant porridges were preferred the most (37.44%). Conclusion: Concerning local infant flour, some of them did not meet WHO guidelines. With regard to food practice indicators in Ouagadougou, some of them are relatively satisfactory; however, there is still a need to raise awareness regarding feeding practices.

Abstract: This paper explores the use of John Searle's directive speech acts as advertising strategies implemented by mobile network companies in Côte d'Ivoire. It describes the way mobile network companies resort to J. Searle’s directive speech acts to manipulate customers or potential customers into subscribing to their services. Through a lens of John Searle’s Speech Act Theory, the study is underpinned in pragmatics and carried out in a qualitative descriptive approach. The analysis of the selected advertisements shows that mobile network companies in Côte d’Ivoire use strategic language to appeal to the needs and desires of customers and potential customers and invite them into specific actions through directive speech acts such as inviting, ordering, and commanding. These findings in turn can empower customers or potential customers into their subscription decisions to mobile services. Keywords: Advertising, Companies, Mobile, Speech, Strategy.

Malaria is the leading cause of morbidity among children under five years of age and pregnant women in Côte d’Ivoire. We assessed the geographical distribution of its risk in all climatic zones of the country based on the Fifth Assessment Report (AR5) of the United Nations Intergovernmental Panel on Climate Change (IPCC) approach to climate risk analysis. This methodology considers three main driving components affecting the risk: Hazard, exposure and vulnerability. Considering the malaria impact chain, various variables were identified for each of the risk factors and for each variable, a measurable indicator was identified. These indicators were then standardized, weighted through a participatory approach based on expert judgement and finally aggregated to calculate current and future risk. With regard to the four climatic zones in the country: Attieen (sub-equatorial regime) in the South, Baouleen (humid tropical) in the centre, Sudanese or equatorial (tropical transition regime) in the North and the mountainous (humid) in the West. Malaria risk among pregnant women and children under 5 was found to be higher in the mountainous and the Baouleen climate, with the hazard highest in the mountainous climate and Exposure very high in the Attieen climate. The most vulnerable districts were those in Baouleen, Attieen and the mountainous climates. By 2050, the IPCC representative concentration pathway (RCP) 4.5 and 8.5 scenarios predict an increase in risk in almost all climatic zones, compared to current levels, with the former considering a moderate scenario, with an emissions peak around 2040 followed by a decline and RCP 8.5 giving the highest baseline emissions scenario, in which emissions continue to rise. It is expected that the AR5 approach to climate risk analysis will be increasingly used in climate risk assessment studies so that it can be better assessed at a variety of scales.

Research on greywater reuse in water stressed areas is in full swing. However, the perception of greywater reuse is one of the least researched areas in West Africa, particularly in Sahelian countries. This study aimed to fills a significant gap in the existing literature, which has largely ignored the specific socio-demographic contexts of developing countries in the Sahelian regions. The study involved in-depth interviews with 240 rural households and the collection of 40 greywater samples in four locations for laboratory analysis. The survey focused on greywater management and household perceptions of greywater reuse in agriculture. The analyses focused on determining the physico-chemical and microbiological parameters of greywater collected from households. The results showed that over 80 % of households discharged greywater into the natural environment without prior treatment. The majority of respondents were aware that poor greywater management poses a health risk. The results also showed an association between locality, gender, education level and perceptions of poor grey water management. Respondents were willing to accept the reuse of greywater in agriculture, to consume irrigated vegetables and to install a greywater treatment system. The quality analysis showed that the greywater was not in compliance with the legal discharge limits. This study highlights that in order to promote sustainable greywater management practices within households, it is important to design effective greywater treatment systems that meet the needs of the target population. Awareness campaigns, education and training programmes on wastewater management could also be established.

Abstract Malaria vaccine development is hampered by extensive antigenic variation and complex life stages of Plasmodium species. Vaccine development has focused on a small number of antigens, many of which were identified without utilizing systematic genome-level approaches. In this study, we implement a machine learning-based reverse vaccinology approach to predict potential new malaria vaccine candidate antigens. We assemble and analyze P. falciparum proteomic, structural, functional, immunological, genomic, and transcriptomic data, and use positive-unlabeled learning to predict potential antigens based on the properties of known antigens and remaining proteins. We prioritize candidate antigens based on model performance on reference antigens with different genetic diversity and quantify the protein properties that contribute most to identifying top candidates. Candidate antigens are characterized by gene essentiality, gene ontology, and gene expression in different life stages to inform future vaccine development. This approach provides a framework for identifying and prioritizing candidate vaccine antigens for a broad range of pathogens.

Abstract Non‐destructive detection of wire bonding defects in integrated circuits (IC) is critical for ensuring product quality after packaging. Image‐processing‐based methods do not provide a detailed evaluation of the three‐dimensional defects of the bonding wire. Therefore, a method of 3D reconstruction and pattern recognition of wire defects based on stereo vision, which can achieve non‐destructive detection of bonding wire defects is proposed. The contour features of bonding wires and other electronic components in the depth image is analysed to complete the 3D reconstruction of the bonding wires. Especially to filter the noisy point cloud and obtain an accurate point cloud of the bonding wire surface, a point cloud segmentation method based on spatial surface feature detection (SFD) was proposed. SFD can extract more distinct features from the bonding wire surface during the point cloud segmentation process. Furthermore, in the defect detection process, a directional discretisation descriptor with multiple local normal vectors is designed for defect pattern recognition of bonding wires. The descriptor combines local and global features of wire and can describe the spatial variation trends and structural features of wires. The experimental results show that the method can complete the 3D reconstruction and defect pattern recognition of bonding wires, and the average accuracy of defect recognition is 96.47%, which meets the production requirements of bonding wire defect detection.

Genetic alterations in the TP63 (GenBank: NC_000003.12, ID: 8626) and CCR5 (receptor 5 chemokine co-receptor) (GenBank: NC_000003.12, ID: 1234) genes may increase the risk of developing breast cancer. The aim of this study was to investigate the probable involvement of polymorphisms rs17506395 in the TP63 (tumour protein 63) gene and the CCR5Δ32 mutation in the occurrence of breast cancer in Burkina Faso. This case–control study included 72 patients and 72 controls. Genotyping of SNP rs17506395 (TP63) was performed by polymerase chain reaction–restriction fragment length polymorphism, and genotyping of the CCR5Δ32 mutation was performed by allele-specific oligonucleotide polymerase chain reaction. For SNP rs17506395 (TP63), the genotypic frequencies of wild-type homozygotes (TT) and heterozygotes (TG) were, respectively, 27.72 and 72.22% in cases and 36.11 and 63.89% in controls. No mutated homozygotes (GG) were observed. For the CCR5Δ32 mutation, the genotypic frequencies of wild-type homozygotes (WT/WT) and heterozygotes (WT/Δ32) were 87.5 and 13.5%, respectively, in the cases and 89.29 and 10.71%, respectively, in the controls. No mutated homozygotes (Δ32/Δ32) were observed. None of the polymorphisms rs17506395 of the TP63 gene (OR = 1.47, 95% CI = 0.69–3.17, P = 0.284) and the CCR5Δ32 mutation (OR = 1.32, 95% CI = 0.46–3.77; P = 0.79) were associated with the occurrence of breast cancer in this study.

Abstract Non-timber forest products are often disregarded in favor of primary crops in Burkina Faso, despite their nutritional significance and contribution to food self-sufficiency. However, the lack of scientific information regarding the amino acid content of Saba senegalensis pulp impedes its utilization in various foods production. Consequently, the primary objective of this investigation was to assess the free amino acid profile of S. senegalensis pulp in the three distinct climatic zones of Burkina Faso. Fruit samples were collected from these climatic regions, and the amino acid content was analyzed using HPLC methods. The analysis of S. senegalensis pulp revealed the presence of essential amino acids in mg/100 g, mainly isoleucine (300 ± 75–305 ± 15), leucine (370 ± 92.50–377 ± 95.50), lysine (200 ± 50–205 ± 70), methionine (90 ± 22.50–104 ± 50), phenylalanine (140 ± 35–196 ± 15), threonine (230 ± 57.50–241 ± 27.50), valine (260 ± 65–285 ± 40), and tryptophan (230 ± 57.50–237 ± 75.50), as well as non-essential amino acids. Statistical analysis indicated no significant difference (p > 0,5) in the essential and non-essential amino acids across the climatic zones. According to the revised model spectrum of ideal essential amino acids for humans by the WHO/FAO, the E/T values ranged from 43.12 to 43.40%, and the E/NE values ranged from 75.83 to 76.68%. The BC/E values ranged from 50.69 to 51.09%, while the BC/A values ranged from 1.24 to 1.37. Principal component analysis (PCA) revealed specific variations in the amino acid composition of the fruit pulp based on the climatic zones. This study demonstrates that S. senegalensis is a valuable source of amino acids and can potentially enhance food security.

During January 28–May 5, 2019, a meningitis outbreak caused by Neisseria meningitidis serogroup C (NmC) occurred in Burkina Faso. Demographic and laboratory data for meningitis cases were collected through national case-based surveillance. Cerebrospinal fluid was collected and tested by culture and real-time PCR. Among 301 suspected cases reported in 6 districts, N. meningitidis was the primary pathogen detected; 103 cases were serogroup C and 13 were serogroup X. Whole-genome sequencing revealed that 18 cerebrospinal fluid specimens tested positive for NmC sequence type (ST) 10217 within clonal complex 10217, an ST responsible for large epidemics in Niger and Nigeria. Expansion of NmC ST10217 into Burkina Faso, continued NmC outbreaks in the meningitis belt of Africa since 2019, and ongoing circulation of N. meningitidis serogroup X in the region underscore the urgent need to use multivalent conjugate vaccines in regional mass vaccination campaigns to reduce further spread of those serogroups.

Background: The emergence of antimicrobial resistance (AMR) within bacterial pathogens necessitates a local comprehension of the epidemiological context. This information is indispensable for both clinical therapeutic determinations and the reevaluation of prevailing care protocols. This study aimed to highlight the antibiotic susceptibility profile of uropathogenic bacteria isolated within a university hospital in Burkina Faso, with a focus on enhancing probabilistic antibiotic therapy for both community and hospital-based urinary tract infections (UTIs). Methodology: Data from cytological urine analysis and antimicrobial susceptibility testing spanning 29 months (January 2017 to May 2019) was retrospectively collected and systematically analyzed. Results: In both hospital and community based UTIs, Enterobacterales dominated, constituting 79.86% (81.27% vs. 79.11%) of isolates. This was followed by non-fermentative Gram-negative bacteria at 6.60% (6.35% vs. 6.88%) and Gram-positive cocci at 6.41% (7.98% vs. 5.57%). Escherichia coli (61.37%), Klebsiella pneumoniae (10.66%), and Enterobacter spp. emerged as the predominant pathogens in the same rank regardless of the origin of the ITUs. Imipenem (97.19%), amikacin (69.26%), ceftriaxone (58.44%), and ciprofloxacin (47.60%) displayed superior susceptibility against all uropathogens. Subtle but significant variations emerged between hospital and community strains' susceptibility to various antibiotics, including amoxicillin + clavulanic acid (26.05% vs. 32.26%), Imipenem (96.43% vs. 98.59%), and ciprofloxacin (45.51% vs. 51.41%). Conclusion: Penicillins showcased diminished efficacy against uropathogens, while resistance to fluoroquinolones escalated. The combined use of aminoglycosides and third-generation cephalosporins holds promise as an optimal probabilistic therapy for UTIs. Notably, the profiles of hospital and community UTIs showed substantial similarities in terms of implicated uropathogens, yet hospital strains demonstrated higher resistance levels.

Islam is the most widespread religion in the world, and Islamic teachings emphasize the importance of seeking knowledge. As a result, Muslims are eager to provide educational opportunities for their children. Islamic schools in countries such as West Africa continue this tradition in modern times. This study aimed to explore the experiences and obstacles encountered by Islamic school leaders in The Gambia and Burkina Faso. It used a qualitative approach, specifically a case study methodology. Five principals from The Gambia participated in the first case study, while three principals from Burkina Faso participated in the second case study.The study revealed several significant findings. It showed that both countries' Islamic schools achieve better educational outcomes than public schools. Additionally, Islamic schools need more support related to funding and low teacher salaries. Both countries also need stronger administrative staff in Islamic schools. Based on the results, the paper presented several recommendations to improve the situation of Islamic schools in The Gambia and Burkina Faso.

Background: The treatment of radial club hands is difficult and requires staged procedures in specialised centres. Objective: To report the Ivorian experience with this condition. Settings: Yopougon Teaching Hospital, Abidjan and "Don Orione” Centre for the Crippled, Bonoua. Subjects and Methods: The management of 22 club hands in a cohort of 14 patients (nine boys and five girls) aged 14 days - 12 years (mean age, 3.3 years + 4.3) over a 14-year period, was reviewed. Three of the children were neonates, six were aged one month-one year while the rest were older than one year. The lesions were classified according to the method described by Bayne & Klug. Theywere all treated surgically with preoperative physical care in the neonates and infants. Functional and aesthetic results were assessed by using the criteria of Cattaneo etal at a mean follow up period of 2.4 years. Results: Twelve of the children had Bayne type IV deformity, nine type I and one type II. Eleven children with type IV deformity were treated by centralization with ulnar osteotomy and one child with type IV, by centralization alone. Complications included one case of wound infection, two with haematomas and two pin migrations without recurrence of wrist deviation. According to Cattaneo et al's staging score, the hand function was good in 18 cases and average in four; aesthetic result was good in 12 hands, average in six and poor in four. Conclusion: The treatment provided satisfactory results for the children and their families with the result that there has since been an increase in the number of club hands referred to the hospital.

BackgroundViral gastrointestinal infections remain a major public health concern in developing countries. In Burkina Faso, there are very limited updated data on the circulating viruses and their genetic diversity.ObjectivesThis study investigates the detection rates and characteristics of rotavirus A (RVA), norovirus (NoV), sapovirus (SaV) and human astrovirus (HAstV) in patients of all ages with acute gastrointestinal infection in urban and rural areas.Study design & methodsFrom 2018 to 2021, stool samples from 1,295 patients with acute gastroenteritis were collected and screened for RVA, NoV, SaV and HAstV. Genotyping and phylogenetic analyses were performed on a subset of samples.ResultsAt least one virus was detected in 34.1% of samples. NoV and SaV were predominant with detection rates of respectively 10.5 and 8.8%. We identified rare genotypes of NoV GII, RVA and HAstV, recombinant HAstV strains and a potential zoonotic RVA transmission event.ConclusionsWe give an up-to-date epidemiological picture of enteric viruses in Burkina Faso, showing a decrease in prevalence but a high diversity of circulating strains. However, viral gastroenteritis remains a public health burden, particularly in pediatric settings. Our data advocate for the implementation of routine viral surveillance and updated management algorithms for diarrheal disease.