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Historic areas are at the forefront of global attention because of their historical and social traits that reflect human civilization, giving them an economic weight that helps preserve the city's historical identity. For that, the aim of this paper is based on examining and analyzing the components of a deteriorated stucco window kept in the Restoration Lab of Bab Al-azab Area of the Citadel of Salah al-din Al-ayyubi, which was carried out via different techniques. The stucco window used in this study was inspected using a digital microscope and scanning electron microscope to determine the degradation of the stucco and wooden frame's outside surface. Besides, the polarized microscope involves studying minerals by studying the optical properties of stucco. Also, the light microscope was used to identify the wood. Besides, energy dispersive X-ray (EDX) showed the presence of constituent elements for both the stucco and glass. In addition, X-ray diffraction estimated cellulose crystallinity to decrease. A technique called Fourier transform infrared (FTIR) was also used to find changes in lignin, gypsum, anhydrite, and calcite, as well as cellulose and hemicellulose. A microbiological investigation indicated the presence of a fungal infestation on a wooden frame. This study showed that Salah El-Din Castle's semi-separate historical areas can be considered one of its architectural, planning, and historical models, which have their fabric and characteristics. • A Perforation technique requiring technical skills was used to make this window. • The stucco window was carefully examined and analyzed via different techniques. • Microbiological investigation indicated the presence of a fungal infestation of a wooden frame. [ABSTRACT FROM AUTHOR]