1. Successful Mycobacterium tuberculosis culture isolation from tongue swabs: Results from both experimentally infected and clinical swabs from pulmonary tuberculosis patients.
- Author
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Rigouts L, Condé M, Hassane-Harouna S, Bah K, Gumusboga M, Ruhwald M, Reenaers R, Fissette K, Gaichiri M, De Vos M, and De Jong BC
- Subjects
- Humans, Bacteriological Techniques methods, Male, Adult, Female, Culture Media chemistry, Middle Aged, Sputum microbiology, Young Adult, Mycobacterium tuberculosis isolation & purification, Mycobacterium tuberculosis growth & development, Tuberculosis, Pulmonary diagnosis, Tuberculosis, Pulmonary microbiology, Tongue microbiology, Specimen Handling methods
- Abstract
This study explored Mycobacterium tuberculosis (MTB) growth from tongue swabs, both experimentally infected after sampling from healthy controls, or sampled from patients with smear-microscopy confirmed pulmonary tuberculosis (PTB). For both, we evaluated the performance of NALC-NaOH/MGIT960 (MGIT), Kudoh-Ogawa (KO), and cetylpyridinium chloride-Löwenstein-Jensen (CPC/LJ) culture processing methods. Experimentally spiked swabs from 20 participants exhibited 94.4% MTB growth when inoculated within 7 days of CPC exposure, declining significantly after 14-21 days (p<0.00001). KO-processed specimens showed 100% MTB growth, with a non-significant reduction after storage (94.1%; p=0.21), and all spiked swabs yielded growth in MGIT. In the field evaluation on 99 PTB patients, MGIT isolated MTB from 89% of tongue swabs, with an 8% contamination rate, compared to 99% MGIT positivity from sputum. Solid media had lower positivity, 62% for KO and 49% for CPC/LJ, suggesting MGIT as optimal for growing MTB from tongue swabs. Further testing of presumptive PTB patients is recommended., Competing Interests: Declaration of competing interest None of the authors have a competing interest in this research to declare., (Copyright © 2024. Published by Elsevier Inc.)
- Published
- 2024
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