1. Effects of Hyperbaric Oxygen Therapy on Hemogram, Serum Biochemistry and Coagulation Parameters of Dogs Undergoing Elective Laparoscopic-Assisted Ovariohysterectomy.
- Author
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Antunes, Bernardo Nascimento, Caye, Pâmela, Schiefler, Otávio Henrique de Melo, Jung, Jenifer, Engelsdorff, João Segura, Tostes, Vitória Pina, Degregori, Emanuelle Bortolotto, Reinstein, Rainer da Silva, De Andrade, Cinthia Melazzo, and Brun, Maurício Veloso
- Subjects
HYPERBARIC oxygenation ,BLOOD cell count ,FEMALE dogs ,HYSTERO-oophorectomy ,LEUKOCYTE count ,BLOOD coagulation factors - Abstract
Simple Summary: Hyperbaric oxygen therapy (HBOT) has broad potential as an adjuvant therapy for various medical and surgical conditions with potential preoperative use in the preservation and/or preparation of the surgical bed. However, further studies on their effects on healthy individuals are required. This study explored the effects of HBOT on hemogram, serum biochemistry and hemostatic parameters in female dogs undergoing laparoscopic-assisted ovariohysterectomy. Thirty adult, mixed-breed, healthy female dogs were randomly separated into three groups: HBOT + SURG group (exposure to two ATAs for 45 min followed by video-assisted OVH), HBOT group (exposure to two ATAs for 45 min) and SURG group (video-assisted OVH). Blood samples were collected at T0 (at the admission), at T1, 24 h after T0 (immediately after HBOT in the HBOT + SURG and HBOT groups, and immediately before anesthetic premedication in the SURG group), and at T2, 48 h after T0 (24 h after HBOT and anesthetic premedication). Laboratory assessments included erythrocyte, leukocyte and platelet count, renal and hepatic serum biochemistry, prothrombin time (PT), activated partial thromboplastin time (APTT), buccal mucosal bleeding time (BMBT) and bloodstain area in a hygroscopic paper (BA). In conclusion, a session of HBOT at two ATAs for 45 min did not cause changes in the BMBT and BA of healthy bitches. Some modifications in leukocyte, neutrophil and lymphocyte count, as well as in alkaline phosphatase, PT and APTT were observed in the different groups considered, mostly dependent on the use of HBOT. Background: This study explored the effects of hyperbaric oxygen therapy (HBOT) on hemogram, serum biochemistry and hemostatic variables in female dogs undergoing laparoscopic-assisted ovariohysterectomy (OVH). Materials: Thirty adult, mixed-breed, healthy female dogs were randomly divided into the following three groups: HBOT + SURG (exposed to two absolute atmospheres (ATAs) for 45 min followed by laparoscopic-assisted OVH), HBOT (exposed to two ATAs for 45 min) and SURG (laparoscopic-assisted OVH). Blood samples were collected at T0 (at the admission), at T1, 24 h after T0 (immediately after HBOT in the HBOT + SURG and HBOT groups, and immediately before anesthetic premedication in the SURG group), and at T2, 48 h after T0 (24 h after HBOT and anesthetic premedication). Methods: Assessments included erythrogram, leukogram, thrombogram, renal and hepatic serum biochemistry, prothrombin time (PT), activated partial thromboplastin time (APTT), buccal mucosal bleeding time (BMBT) and bloodstain area (BA) on hygroscopic paper collected at the BMBT. Results: Both the HBOT + SURG and SURG groups presented neutrophilia (p ≤ 0.0039) at T2 and an increase of ALP at T2 (p ≤ 0.0493), the SURG group presented an increase in leukocyte count at T2 (p = 0.0238) and the HBOT + SURG group presented a reduction in lymphocyte count at T2 (p = 0.0115). In the HBOT + SURG group, there was a reduction in PT and APTT in relation to the baseline value (p ≤ 0.0412). Conclusions: A session of HBOT at two ATAs for 45 min did not cause changes in the BMBT or BA in healthy female dogs. Some blood parameters investigated (neutrophil and lymphocyte count, ALP, PT and APTT) were affected by the use of HBOT. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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