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Humus materials are considered complex organic substances generated through a chain of chemical reactions and responsible for many processes in soil that ultimately govern soil health. The structural and functional characteristics of humus depend upon the location, quality, and microbial abundance of the soils. However, the differential characteristics of soil organic matter, seasonal changes, parent rock, plant cover, microbial abundance, and anthropogenic activities majorly affect it. The present study has aimed toward the extraction of humus from five different locations in the Delhi region of India and their characteristics were investigated through elemental analysis, Fourier Transform infrared (FT-IR) spectroscopy, and UV spectroscopy. The results showed that there was a higher degree of unsaturation detected in the Forest soil sample. The results of FT-IR showed the presence of characteristic peaks of humus in the samples however the intensity of bands was weak in sample disposable site soil sample and clayey soil sample due to the variation in soil physicochemical properties. The study also aimed to assess the growth of Oryza sativa (rice) plants observed in the hydroponics system. The significant finding was observed with the forest soil sample in 1000 mgL-1 and treatment in which the growth was minimum in clayey soil of 1500 mgL-1. Our investigation infers the diverse nature of humus in different soils and its implications for plant growth, highlighting the importance of understanding soil organic matter for sustainable agriculture and soil health management. [ABSTRACT FROM AUTHOR]