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Sulfur (VI) fluoride exchange (SuFEx), a new generation of click chemistry, enables the creation of a stable covalent linkage between a protein and its interacting biomolecule. This transformative process converts the transient and reversible protein-biomolecule interaction into a stable binding complex, which allows the subsequent pull-down assay to identify unknown interactors. Therefore, SuFEx has emerged as a versatile tool to investigate protein-biomolecule interactions and facilitate new therapeutic development. SuFEx warheads such as aryl fluorosulfates and aryl sulfonyl fluorides can be appended to small molecules to investigate protein-ligand interactions. Furthermore, they can be incorporated into proteins site-specifically to probe protein-protein interactions (PPIs) and protein-RNA interactions with pinpoint accuracy. Here, we describe the principles of SuFEx, summarize its application in profiling protein-biomolecule interactions, and delve into the kinetics of SuFEx. This overview can serve as a roadmap to understanding the application of SuFEx in biomedical research. [ABSTRACT FROM AUTHOR]

At present, there are few studies considering both nonlinear and fractional characteristics of suspension in vehicle systems. In this paper, a fractional nonlinear model of a quarter vehicle with two-degree-of-freedom (2-DOF) is innovatively proposed to describe the suspension system containing the viscoelastic material metal rubber. Given the lack of a general calculation scheme for the multi-degree-of-freedom fractional-order incremental harmonic balance method (IHBM), a general calculation scheme for the 2-DOF incremental harmonic balance method for nonlinear systems with fractional order is derived. The nonlinear dynamical properties of the presented model are acquired using this method. The accuracy of the proposed method is verified through a comparison with the power series expansion method. Afterward, the effects of the various parameters on the dynamic performance are analyzed. The vibration peak value of the fractional-order model is significantly higher than that of the integer-order model (IOM). Therefore, the suspension parameters should be designed with a margin when using IOM. [ABSTRACT FROM AUTHOR]

Wet oxidation absorption is an efficient and promising method of exhaust gas treatment. When the micro-nano bubbles collapse, they can generate hydroxyl radicals with strong oxidising ability, which can oxidise a variety of pollutants in diesel exhaust. Ozone has strong oxidising properties, and the coupling of ozone and micro-nano bubbles can improve the oxidation and removal effects of polluted gases. In this study, the ozone micro-nano bubbles system was used to oxidise NOx, SO2, and CO to gases that were more readily dissolved in water, such as NO2, SO3, and CO2, and the gases were removed through the absorbent solution. The effects of surfactant, catalyst, urea, pH value, and salinity on the removal efficiency of NOx, SO2, and CO from diesel exhaust were investigated. Through experiments, it was found that the removal efficiency of pollutants was enhanced and then weakened with the increasing concentrations of surfactants, catalysts, and salinity, and continued to decrease with increasing concentrations of urea. When the pH value was < 7, the removal efficiency increased first and then weakened with the increase of the pH value. When the pH value was > 7, it mainly depended on the absorption of acid gas by the alkali solution. Under optimal conditions, the removal efficiencies were 86.3% for NO, 92.1% for SO2, and 65.4% for CO. This study could provide important theoretical support for the industrial application of this technology. [ABSTRACT FROM AUTHOR]

Persimmon anthracnose, a severe disease caused by the hemibiotrophic fungus Colletotrichum horii, poses a substantial threat to China's persimmon industry. Previous research showed that 'Kangbing Jianshi' cultivar exhibits strong resistance to anthracnose. Notably, 'Kangbing Jianshi' branches exhibit greater lignification compared with the susceptible 'Fuping Jianshi' cultivar. In this study, higher lignin content was observed in 'Kangbing Jianshi' compared with 'Fuping Jianshi', and this difference was associated with disease resistance. Transcriptome and metabolome analyses revealed that the majority of differentially expressed genes and differentially accumulated metabolites were primarily enriched in the phenylpropanoid biosynthesis and lignin synthesis pathways. Furthermore, significant upregulation of DkCAD1, a pivotal gene involved in lignin metabolism, was observed in the resistant cultivar when inoculated with C. horii. Transient overexpression of DkCAD1 substantially increased lignin content and improved resistance to C. horii in a susceptible cultivar. Furthermore, through yeast one-hybrid (Y1H) assays, we identified two WRKY transcription factors, DkWRKY8 and DkWRKY10, which interacts with the DkCAD1 promoter and induces its activity. Overexpression of DkWRKY8 and DkWRKY10 not only increased leaf lignin content but also enhanced persimmon tolerance to C. horii. Moreover, the expression levels of DkCAD1, DkWRKY8, and DkWRKY10 were significantly increased in response to salicylic acid and jasmonic acid in the resistant cultivar. These findings enhance our understanding of the molecular functions of DkWRKY8, DkWRKY10, and DkCAD1 in persimmons, as well as their involvement in molecular breeding processes in persimmons. [ABSTRACT FROM AUTHOR]

Petioles of non-heading Chinese cabbage are not only an important edible part but also a conduit for nutrient transport, holding significant agricultural and research value. In this study, we conducted a comprehensive genetic analysis of petiole-related traits using a segregating population. Modern quantitative genetic approaches were applied to investigate the genetic regulation of petiole thickness. The results indicated that petiole thickness is a quantitative trait, and the identified genetic model was consistent with two pairs of additive-dominant main genes and additive-dominant polygenes (2MG-AD). BSA-seq analysis identified a major effect of QTL controlling petiole thickness on chromosome A09: 42.08–45.09 Mb, spanning 3.01 Mb, designated as QTL-BrLH9. Utilizing InDel markers, the interval was narrowed down to 51 kb, encompassing 14 genes with annotations for 10 of them. Within the interval, four mutated genes were detected. Combined with gene annotation, protein sequence analysis, and homology alignment, it was found that BraA09g063520.3C's homologous gene SMXL6 in Arabidopsis (Arabidopsis thaliana (L.) Heynh) is an inhibitor of the coding and synthesis of the strigolactone pathway. Strigolactone (SLs) plays an important role in plant growth and development. The cloning results showed that multiple frameshift mutations and non-synonymous mutations occurred on the exon. The qPCR results showed that the expression of the gene was significantly different between the two parents at the adult stage, so it was speculated that it would lead to changes in petiole thickness. BraA09g063520.3C was predicted as the final candidate gene. [ABSTRACT FROM AUTHOR]