10 results on '"Lelli, Davide"'
Search Results
2. Development of two competitive ELISAs based on monoclonal antibodies for the serological detection of Bovine ephemeral fever virus
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Benevenia, Roberto, Lelli, Davide, Moreno, Ana, Lavazza, Antonio, Kapri-Pardes, Einat, Klement, Eyal, Golender, Natalia, Gleser, Dan, Corsa, Manuel, Castelli, Anna, and Pezzoni, Giulia
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- 2024
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3. Isolation and Molecular Evidence of Tunisian Sheep-like Pestivirus (Pestivirus N) in Persistently Infected Sheep in Northern Italy, 2023.
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Sozzi, Enrica, Leo, Gabriele, Lamcja, Fatbardha, Lazzaro, Massimiliano, Salogni, Cristian, Lelli, Davide, Bertasio, Cristina, Magagna, Giulia, Moreno, Ana, Alborali, Giovanni Loris, Bazzucchi, Moira, and Lavazza, Antonio
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CLASSICAL swine fever virus ,PESTIVIRUS diseases ,SHEEP ,LAMBS ,TUNISIANS ,SHEEP breeds - Abstract
Over the last few decades, several pestiviruses have been discovered in ruminants, pigs, and, more recently, in non-ungulate hosts. Consequently, the nomenclature and taxonomy of pestiviruses have been updated. The Tunisian sheep-like pestivirus (TSV, Pestivirus N) is an additional ovine pestivirus genetically closely related to classical swine fever virus (CSFV). In this study, during a survey of pestivirus infections in ovine farms in the Lombardy region of Northern Italy, we identified and isolated a pestivirus strain from a sheep that was found to belong to Pestivirus N species based on its genomic nucleotide identity. The sheep itself and its lamb were found to be persistently infected. We performed molecular characterization and phylogenetic analysis of three viral genomic regions (a fragment of 5′-UTR, partial N
pro , and the whole E2 region). In conclusion, these results confirmed circulating TSV in Northern Italy after notification in Sicily, Italy, and France. Correlation with Italian, Tunisian, and French strains showed that detection might have resulted from the trading of live animals between countries, which supports the need for health control measures. [ABSTRACT FROM AUTHOR]- Published
- 2024
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4. Strategic Challenges to the Eradication of African Swine Fever Genotype II in Domestic Pigs in North Italy.
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Pavone, Silvia, Bellini, Silvia, Iscaro, Carmen, Farioli, Marco, Chiari, Mario, Lavazza, Antonio, Ruocco, Luigi, Lelli, Davide, Pintus, Giorgia, Prati, Paola, and Feliziani, Francesco
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AFRICAN swine fever ,SWINE ,SWINE farms ,AFRICAN swine fever virus ,WILD boar ,VIRUS diseases - Abstract
Simple Summary: African swine fever (ASF) is a highly lethal viral disease affecting suids and caused by the African Swine Fever Virus (ASFV). ASF was described for the first time in 1921 in Kenya. The ASF genotype I virus was introduced to Europe in 1957, marking the onset of the first European wave. In 2007, ASFV genotype II was detected in Georgia, affecting domestic pigs and wild boars, and it later spread to several European and extra-European countries, including Italy. This report focuses on the strategic challenges encountered in the attempt to eradicate ASFV amongst domestic pigs in the Lombardy region. The joint efforts that were implemented facilitated the eradication of ASF in domestic pigs in just over 1.5 months, representing an example of effective and timely cooperation to mitigate both the spread of the infection and the economic repercussions for the Italian and global pig industries. African swine fever (ASF) is a severe viral disease characterized by high lethality in suids and caused by the African Swine Fever Virus (ASFV). The ASF genotype I virus was introduced to Europe in 1957, marking the onset of the first European epidemic wave. In 2007, ASFV genotype II was detected in Georgia, affecting domestic pigs and wild boars before spreading to various European and extra-European countries, including Italy. The first case of ASFV in Italy was documented on 7 January 2022, in a wild boar in the Piedmont region. Since then, several ASFV-positive wild boar carcasses have been identified in the Piedmont and Liguria regions. By June 2023, ASFV had spread to Lombardy, one of the major pig-producing regions in northern Italy; the virus was first detected in early summer in wild boar carcasses. Two months later, it was diagnosed in a commercial pig farm as a consequence of the disease's spread amongst wild boars and an increase in the viral environmental load. This report aims to describe the features of ASFV domestic pig outbreaks that occurred in the Zinasco municipality (Lombardy) and the joint efforts to mitigate potential direct and indirect economic impacts on the Italian and global pig industry. The epidemiological investigation and the measures implemented, which were all performed according to national and European regulations, as well as exceptional ad hoc measures aimed at protecting the pig industry, are described in order to provide a practical and effective approach to combating ASF. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Molecular and Serological Detection of Bovine Coronaviruses in Marmots (Marmota marmota) in the Alpine Region.
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Moreno, Ana, Canziani, Sabrina, Lelli, Davide, Castelli, Anna, Bianchi, Alessandro, Bertoletti, Irene, Maccarinelli, Federica, Carlomagno, Marco, Paini, Matteo, Rossato, Marzia, Delledonne, Massimo, Giacomelli, Stefano, Cordedda, Antonella, Nicoloso, Sandro, Bellinello, Enrica, Campagnoli, Anna, and Trogu, Tiziana
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ALPINE regions ,CORONAVIRUSES ,SENDAI virus ,CANINE distemper virus ,WHOLE genome sequencing ,ROE deer ,FORAGE - Abstract
In this study, virological surveillance focused on coronaviruses in marmots in the Alpine region in 2022, captured as part of a population control reduction program in the Livigno area. Seventy-six faecal samples were randomly collected from marmots at the time of capture and release and tested for genome detection of pan-coronavirus, pan-pestivirus, canine distemper virus, and influenza A and D virus. Nine faecal samples were positive in the Pan-CoV RT-PCR, while all were negative for the other viruses. Pan-coronavirus positives were further identified using Illumina's complete genome sequencing, which showed the highest homology with Bovine Coronavirus previously detected in roe deer in the Alps. Blood samples (n.35) were collected randomly from animals at release and tested for bovine coronavirus (BCoV) antibodies using competitive ELISA and VNT. Serological analyses revealed that 8/35 sera were positive for BCoV antibodies in both serological tests. This study provides molecular and serological evidence of the presence of BCoV in an alpine marmot population due to a likely spillover event. Marmots share areas and pastures with roe deer and other wild ruminants, and environmental transmission is a concrete possibility. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Characterization of environmental drivers influencing the abundance of Anopheles maculipennis complex in Northern Italy.
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Gilioli, Gianni, Defilippo, Francesco, Simonetto, Anna, Heinzl, Alessandro, Migliorati, Manlio, Calzolari, Mattia, Canziani, Sabrina, Lelli, Davide, and Lavazza, Antonio
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ANOPHELES ,MOSQUITO control ,SHRUBLANDS ,POPULATION density ,ANIMAL shelters ,LAND cover ,CURRENT distribution ,SOIL sampling - Abstract
Background: In Italy, malaria was endemic until the 1970s, when it was declared eradicated by WHO. Nowadays, with the persistence of competent mosquito populations, the effect of climate change, and increased possibility of importing malaria parasites from endemic counties due to growing migration, a malaria resurgence in Italy has become more likely. Hence, enhancing the understanding of the current distribution of the Anopheles maculipennis complex and the factors that influence the presence of this malaria vector is crucial, especially in Northern Italy, characterised by a high density of both human population and livestock. Methods: To assess the presence and abundance of malaria vectors, a 4-year field survey in the plain areas of Lombardy and Emilia-Romagna region in Italy was conducted. Every sampling point was characterised in space by the land use in a 500-m radius and in time considering meteorological data collected in the short and long time periods before sampling. We combined the results of a linear regression model with a random forest analysis to understand the relative importance of the investigated niche dimensions in determining Anopheles mosquito presence and abundance. Results: The estimated normalised variable importance indicates that rice fields were the most important land use class explaining the presence of Anopheles, followed by transitional woodlands and shrubland. Farm buildings were the third variable in terms of importance, likely because of the presence of animal shelters, followed by urbanised land. The two most important meteorological variables influencing the abundance of Anopheles in our study area were mean temperature in the 24 h before the sampling date and the sum of degree-days with temperature between 18 °C and 30 °C in the 14 days before the sampling date. Conclusions: The results obtained in this study could be helpful in predicting the risk of autochthonous malaria transmission, based on local information on land cover classes that might facilitate the presence of malaria vectors and presence of short- and medium-term meteorological conditions favourable to mosquito development and activity. The results can support the design of vector control measures through environmental management. [ABSTRACT FROM AUTHOR]
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- 2024
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7. First Reported Circulation of Equine Influenza H3N8 Florida Clade 1 Virus in Horses in Italy.
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Ricci, Ida, Tofani, Silvia, Lelli, Davide, Vincifori, Giacomo, Rosone, Francesca, Carvelli, Andrea, Diaconu, Elena Lavinia, La Rocca, Davide, Manna, Giuseppe, Sabatini, Samanta, Costantini, Donatella, Conti, Raffaella, Pacchiarotti, Giulia, and Scicluna, Maria Teresa
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EQUINE influenza ,VIRUS diseases ,INFLUENZA A virus ,HORSE breeding ,COMMUNICABLE diseases ,HORSES ,INFLUENZA viruses - Abstract
Simple Summary: Equine influenza (EI) is an acute and highly contagious viral disease of equids characterized by fever and respiratory signs. Ongoing antigenic mutations that are typical of influenza viruses, which may cause a reduction in the effectiveness of vaccines, highlight how crucial both surveillance and virus characterization are for updating vaccine compositions. The aim of this study was to verify the identity of the equine influenza virus strains detected in Italy, especially in the absence of formal active surveillance. Twenty nasal swabs, collected from symptomatic horses located in North and Central Italy between February and April 2019 were positive for influenza A virus (IAV) RRT-PCR. Sequencing identified an isolated strain as H3N8 Florida lineage clade 1 for one sample from Brescia, Lombardy Region. This study is the first report of H3N8 Florida lineage clade 1 circulation in Italy, confirming the value of monitoring for EIV circulating strain in relation to the appropriateness of the vaccine virus composition for maximum efficacy. Background: Equine influenza (EI) is a highly contagious viral disease of equids characterized by pyrexia and respiratory signs. Like other influenza A viruses, antigenic drift or shift could lead to a vaccine-induced immunity breakdown if vaccine strains are not updated. The aim of this study was to genetically characterize EIV strains circulating in Italy, detected in PCR-positive samples collected from suspected cases, especially in the absence of formal active surveillance. Methods: Between February and April 2019, blood samples and nasal swabs collected from each of the 20 symptomatic horses from North and Central Italy were submitted to the National Reference Centre for Equine Diseases in Italy to confirm preliminary analysis performed by other laboratories. Results: None of the sera analysed using haemagglutination inhibition and single radial haemolysis presented a predominant serological reactivity pattern for any antigen employed. All nasal swabs were positive with IAV RRT-PCR. Only one strain, isolated in an embryonated chicken egg from a sample collected from a horse of a stable located in Brescia, Lombardy, was identified as H3N8 Florida lineage clade 1 (FC1). In the constructed phylogenetic trees, this strain is located within the FC1, together with the virus isolated in France in 2018 (MK501761). Conclusions: This study reports the first detection of H3N8 FC1 in Italy, highlighting the importance of monitoring circulating EIV strains to verify the vaccine composition appropriateness for maximum efficacy. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Tick-Borne Encephalitis, Lombardy, Italy.
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Gaffuri, Alessandra, Sassera, Davide, Calzolari, Mattia, Gibelli, Lucia, Lelli, Davide, Tebaldi, Alessandra, Vicari, Nadia, Bianchi, Alessandro, Pigoli, Claudio, Cerioli, Monica, Zandonà, Luca, Varisco, Giorgio, Bertoletti, Irene, and Prati, Paola
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TICK-borne encephalitis ,ENCEPHALITIS viruses ,SYMPTOMS - Abstract
Tick-borne encephalitis was limited to northeast portions of Italy. We report in Lombardy, a populous region in the northwest, a chamois displaying clinical signs of tickborne encephalitis virus that had multiple virus-positive ticks attached, as well as a symptomatic man. Further, we show serologic evidence of viral circulation in the area. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Presence and Characterisation of Porcine Respirovirus 1 (PRV1) in Northern Italy.
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Sozzi, Enrica, Leo, Gabriele, Bertasio, Cristina, Alborali, Giovanni Loris, Salogni, Cristian, Tonni, Matteo, Formenti, Nicoletta, Lelli, Davide, Moreno, Ana, Trogu, Tiziana, Canziani, Sabrina, Tolini, Clara, Cerioli, Monica Pierangela, and Lavazza, Antonio
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PORCINE reproductive & respiratory syndrome ,SWINE influenza ,RESPIRATORY diseases ,PARAMYXOVIRUSES ,RNA viruses ,SWINE farms - Abstract
Porcine Respirovirus 1 (PRV1) is an enveloped, single-stranded, negative-sense RNA virus belonging to the genus Respirovirus within the Paramyxoviridae family. Since its first detection in China in 2013, PRV1 has been identified in several American and European countries. Although its pathogenicity is uncertain, recent studies have suggested that it may play a role in the Porcine Respiratory Disease Complex (PRDC) because of its capacity to replicate in the upper and lower respiratory tracts. This study aimed to determine the spread of PRV1 in Northern Italy and the phylogeny of the isolates. Therefore, PRV1 was investigated using real-time RT-PCR in 902 samples collected from September 2022 to September 2023 from pigs with respiratory symptoms in North Italy. Fourteen (1.55%) samples tested as PRV1-positive. The full-length fusion (F) gene, which codifies for a major surface protein, was amplified and used for phylogenetic analysis to help carry out molecular epidemiological studies on this virus. In addition, swine influenza virus (SIV) and porcine reproductive and respiratory syndrome virus (PRRSV) infections were detected in most of the PRV1-positive samples. In conclusion, we report the detection of PRV1 in Italy and discuss its potential role as a co-factor in causing the Porcine Respiratory Disease Complex. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Identification of asinine gamma herpesviruses in a donkey with interstitial pulmonary fibrosis, pleural effusion and thrombocytopenia.
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Imposimato, Ilaria, Muscatello, Luisa Vera, Ellero, Nicola, Lelli, Davide, Mira, Francesco, Sarli, Giuseppe, and Freccero, Francesca
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• Severe chronic respiratory signs could be due to pulmonary fibrosis in donkeys. • Gammaherpesviruses (GHVs) can be associated with interstitial pulmonary fibrosis. • Asinine GHVs were identified postmortem in a donkey with pulmonary fibrosis. • Histology is needed for the confirmatory diagnosis of pulmonary fibrosis. A 23-year-old domestic donkey (Equus asinus) referred for severe respiratory distress due to suspected equine asthma. Ultrasound of the chest revealed bilateral irregular pulmonary consolidation and pleural effusion. Airway endoscopy and tracheal wash cytology showed severe neutrophilic inflammation and bacterial culture was positive for Streptococcus equi subsp. zooepidemicus. Despite aggressive treatment, the donkey died in 48 hours. On post-mortem examination, the lung was whitish, collapsed, and firm, with fibrotic multifocal nodular areas. Pleural effusion and pleuritis were detected. Histologically, the lung architecture was markedly replaced by interstitial fibrosis. The histological features observed were suggestive of a severe chronic fibrosing interstitial pleuropneumonia with type 2 pneumocyte hyperplasia and intralesional syncytial cells. Pulmonary fibrosis was associated with the presence of asinine gammaherpesvirus 2 and 5 infection, confirmed by PCR and sequence analysis. The macroscopic and histological pattern of fibrosis was diffuse and interstitial, and the nodular lesions were consistent with spared lung parenchyma, instead of the canonical nodular distribution of the fibrosis observed in equine multinodular pulmonary fibrosis. Asinine herpesviral pulmonary fibrosis is uncommon, but should be considered by clinicians in the list of differentials in donkeys with chronic respiratory signs. [ABSTRACT FROM AUTHOR]
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- 2024
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