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Angola, a country grappling with prevalent tropical diseases such as malaria, is witnessing an alarming rise in cancer-related deaths. Despite the escalating significance of cancer globally and in Angola, the nation's medical schools lack compulsory oncology disciplines in their curricula. This absence compromises the comprehensive training of medical students, preventing the development of integrated perspectives and skills crucial for addressing the growing cancer burden. This article, authored by the Angolan Oncology Research Group (AORG), proposes an oncology program for undergraduate medical students in Angola, aiming to bridge the educational gap. The program outlines discipline objectives, topics to be covered, class formats, and workload considerations. [ABSTRACT FROM AUTHOR]

Hyptis suaveolens (L.) Poit is known for containing podophyllotoxin (PTOX), a natural lignan used as the lead compound in antitumor agent preparations, in its roots. The use of phloroglucinol (PHL) and indoleamines, such as melatonin (MEL) and serotonin (SER), in plant tissue culture is a promising alternative for eliciting rare and high-value secondary metabolites of medical importance. Aimed to evaluate the impact of different concentrations of PHL, either alone or in combination with MEL or SER, on the growth and accumulation of PTOX in H. suaveolens. Apical segments were vertically inoculated in MS medium containing different concentrations of MEL or SER (0; 0.25; 0.50; 1.00; 5.00 and 10.00 µM) and PHL (0 and 50 mg L-1). After 40 days, growth parameters, chlorophyll content and PTOX content were assessed. Plantlet rooting and root dry weight notably increased with the use of 0.5 µM MEL alongside PHL. Overall, treatments containing PHL showed superior results, except for chlorophyll content. SER supplementation was effective in increasing root growth, root number and leaf area. The combination of 1.0 µM MEL or SER and 50 mg L-1 PHL increased the production of PTOX in the roots of H. suaveolens by 2.0 and 1.6 times, respectively. In conclusion, the application of PHL and indoleamines demonstrated an eliciting effect on both growth and PTOX production in H. suaveolens roots, presenting a promising avenue for further research and application in eliciting targeted secondary metabolites.Key message: The application of phloroglucinol and indoleamines, such as melatonin (MEL) and serotonin (SER), demonstrated an eliciting effect on both growth and podophylotoxin production in Hyptis suaveolens roots. [ABSTRACT FROM AUTHOR]

This study investigates the impact of three key variables on the performance of nanoporous AM-3 and layered AM-4 titanosilicates in removing nine REEs (Y, La, Ce, Pr, Nd, Eu, Gd, Tb, and Dy) from natural mineral water and identifies optimal operational conditions using Response Surface Methodology (RSM). The experimental conditions were determined by a Box-Behnken Design of 3 factors-3 levels (pH 4, 6, and 8; sorbent dose 20, 100, and 180 mg/L; and element concentration 1, 3, and 5 μmol/L). Three-dimensional response surfaces were used to assess the linear, quadratic, and interaction influences of each factor on the REEs' removal percentage. The pH was the most significant factor in the removal process using AM-3, while the sorbent dose was more important for AM-4. The results highlighted the sorbents' strong capacity for REE removal. The optimal operating conditions obtained by RSM were applied to aqueous solutions with salinity 10 (common in coastal and transitional systems) and 30 (average seawater salinity). The results showed that AM-3 has a strong potential for removing REEs in solutions with salinity 10 and 30, while AM-4 was less efficient due to competition between REEs and other ions present in the solution. [ABSTRACT FROM AUTHOR]

Type 2 diabetes mellitus (T2DM) has been shown to affect a series of cognitive processes including memory, increasing the risk for dementia, particularly Alzheimer's disease (AD). Although increasing evidence has supported that both diseases share common features, the pathophysiological mechanisms connecting these two disorders remain to be fully elucidated. Herein, we used Drosophila melanogaster fed on a high-sugar diet (HSD) to mimic T2DM, and investigate its effects on memory as well as identify potential molecular players associated with the memory deficits induced by HSD. Flies hatched from and reared on HSD for 7 days had a substantial decrease in short-term memory (STM). The screening for memory-related genes using transcriptome data revealed that HSD altered the expression of 33% of memory genes in relation to the control. Among the differentially expressed genes (DEGs) with a fold change (FC) higher than two, we found five genes, related to synapse and memory trace formation, that could be considered strong candidates to underlie the STM deficits in HSD flies: Abl tyrosine kinase (Abl), bruchpilot (Brp), minibrain (Mnb), shaker (Sh), and gilgamesh (Gish). We also analyzed genes from the dopamine system, one of the most relevant signaling pathways for olfactory memory. Interestingly, the flies fed on HSD presented a decreased expression of the Tyrosine hydroxylase (Ple) and Dopa decarboxylase (Ddc) genes, signals of a possible dopamine deficiency. In this work, we present promising biomarkers to investigate molecular networks shared between T2DM and AD. [ABSTRACT FROM AUTHOR]

Hypoxia plays a significant role in the development of various cerebral diseases, many of which are associated with the potential risk of recurrence due to mitochondrial damage. Conventional drug treatments are not always effective for hypoxia-related brain diseases, necessitating the exploration of alternative compounds. In this study, we investigated the potential of diphenyl diselenide [(PhSe)2] to ameliorate locomotor impairments and mitigate brain mitochondrial dysfunction in zebrafish subjected to hypoxia. Additionally, we explored whether these improvements could confer resistance to recurrent hypoxia. Through a screening process, an appropriate dose of (PhSe)2 was determined, and animals exposed to hypoxia received a single intraperitoneal injection of 100 mg/kg of the compound or vehicle. After 1 h from the injection, evaluations were conducted on locomotor deficits, (PhSe)2 content, mitochondrial electron transport system, and mitochondrial viability in the brain. The animals were subsequently exposed to recurrent hypoxia to assess the latency time to hypoxia symptoms. The findings revealed that (PhSe)2 effectively crossed the blood–brain barrier, attenuated locomotor deficits induced by hypoxia, and improved brain mitochondrial respiration by modulating complex III. Furthermore, it enhanced mitochondrial viability in the telencephalon, contributing to greater resistance to recurrent hypoxia. These results demonstrate the beneficial effects of (PhSe)2 on both hypoxia and recurrent hypoxia, with cerebral mitochondria being a critical target of its action. Considering the involvement of brain hypoxia in numerous pathologies, (PhSe)2 should be further tested to determine its effectiveness as a potential treatment for hypoxia-related brain diseases. [ABSTRACT FROM AUTHOR]

Light is one of the factors that influence the growth, development and synthesis of secondary metabolites of different plant species in vitro. Origanum majorana is an aromatic plant belonging to a group of plants that produce arbutin, a substance of importance in the cosmetic industry. The objective of this study was to verify the effect of light intensity and wavelength on the growth and accumulation of arbutin in vitro in plantlets. Nodal segments were grown in MS culture medium under photon flux densities of 26, 51, 69, 94 and 130 µmol m−2 s−1. In the light spectrum, nodal segments were grown under different light-emitting diodes LED: red; blue; a combination of blue and red light (30% B:70% R; 50% B:50% R; 70% B:30% R); and white, yellow and green. After 40 days, the growth, photosynthetic pigments and arbutin accumulation were evaluated by HPLC-DAD. The lowest light intensity (26 µmol m−2 s−1) provided a higher content of photosynthetic pigments, and the intensity of 94 µmol m−2 s−1 increased dry biomass accumulation. Greater accumulation of arbutin was observed under a higher light intensity of 130 µmol m−2 s−1. Regarding the quality of light, different wavelengths did not significantly affect the number of shoots or shoot length. The white light spectrum stimulated a greater accumulation of dry biomass. The green light provided a greater accumulation of photosynthetic pigments. The amount of arbutin was influenced by the different light spectra. The red spectrum promoted the highest arbutin content, and the yellow inhibited.Key message: The intensity of 94 µmol m−2 s−1 increased dry biomass and 130 µmol m−2 s−1 increased arbutin accumulation. The red spectrum promoted the highest arbutin content, and the yellow inhibited. [ABSTRACT FROM AUTHOR]

This paper presents the C-ORAL-ESQ corpus project, which is dedicated to the study of the speech of individuals with schizophrenia. The main aim of the project is to investigate cognitive aspects of individuals with schizophrenia. This investigation is carried through the compilation of a spontaneous speech corpus and its study, which focuses mainly on the analysis of information structuring and its prosodic correlates. The paper mainly deals with the methodological aspects of the corpus compilation and reports its present stage: it informs about the environment and the setting of the sound file recordings, the medical and ethical criteria for the selection of the participants, the corpus aimed dimensions and the present stage of compilation, as well as its design and compilation criteria, which include attention to prosodic annotation, and metadata related to the participants' characteristics. Additionally, the theory adopted for the study of information structure is summarized, focusing on those aspects that can better address cognitive processes of individuals with schizophrenia and their prosodic correlates. Finally, the perspectives for future studies and resource compilations are presented. [ABSTRACT FROM AUTHOR]

High-speed railway bridges are subjected to normative limitations concerning maximum permissible deck accelerations. For the design of these structures, the European norm EN 1991-2 introduces the high-speed load model (HSLM)—a set of point loads intended to include the effects of existing high-speed trains. Yet, the evolution of current trains and the recent development of new load models motivate a discussion regarding the limits of validity of the HSLM. For this study, a large number of randomly generated load models of articulated, conventional, and regular trains are tested and compared with the envelope of HSLM effects. For each type of train, two sets of 100,000 load models are considered: one abiding by the limits of the EN 1991-2 and another considering wider limits. This comparison is achieved using both a bridge-independent metric (train signatures) and dynamic analyses on a case study bridge (the Canelas bridge of the Portuguese Railway Network). For the latter, a methodology to decrease the computational cost of moving loads analysis is introduced. Results show that some theoretical load models constructed within the stipulated limits of the norm can lead to effects not covered by the HSLM. This is especially noted in conventional trains, where there is a relation with larger distances between centres of adjacent vehicle bogies. [ABSTRACT FROM AUTHOR]