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Objective To investigate the differences in gut flora between infants and children aged 0 〜3 years with atopic dermatitis (AD) and healthy infants and children. Methods General information and fecal samples were collected from children with AD aged 0-3 years (AD group) and healthy infants and children (HC group). T Test and Chi 一 Square Tests were used to analyze the general information of infants and children in two groups, and 16S rRNA sequencing technology combined with bioinfbrmatics analysis to compare the diversity and differences of intestinal flora in two groups. Results General information only history of allergy in the immediate family was significantly different between the two groups (丸？= 13. 875, P < 0. 001). The difference in the diversity of Gut microbiota between the two groups was not statistically significant At the gate level, the relative abundance of Verrucomicrobiota was significantly lower in the AD group than in the HC group (P =0. 027), whereas the relative abundance of Fusobacteriota was significantly higher than in the HC group (P = 0. 038). At the genus level, the relative abundance of the genera Cetobacterium (P = 0.010), Dysgonomonas (P = 0.013), and Fusicatenibacter (P = 0. 044) in the AD group was significantly higher than that in the HC group, while the relative abundance of the genera Actinomyces (P = 0. 029), Collinsella (P = 0. 017) and Eggerthella (P = 0. 028) were significantly lower in relative abundance than the HC group. LEfSe analysis showed that the relative abundance of Coriobacteriaceae (P = 0. 017) and Bifidobacterium_longum (P = 0. 002) in the AD group was significantly lower than that in the HC group. Conclusion There are some differences in the composition of the Gut microbiota between the AD and HC groups. Differential microorganisms such as Fusicatenibacter, Eggerthellay Actinomyces and Bifidobacterium ngum may have a correlation with AD development, suggesting that regulating the Gut microbiota early in life may prevent and treat AD. [ABSTRACT FROM AUTHOR]

Objective Based on the health big data platform of Yichang city, to analyze the characteristics of allergic diseases of children born in medical institutions from 2017 to 2022 in Yichang city, and to clarify the distribution of allergic diseases among children in Yichang city. Methods Statistical analysis was conducted based on the records of born children with allergic diseases in the outpatient clinic of urban comprehensive medical institutions from 2017 to 2022 in the big data cloud platform of Yichang city. Results The number of visits of male children was 68 707, more than that of female children, and the number of visits had been increasing since 2017. The highest number of hospital visits was 46 190 (37.86%) among children of 0-1 year old, of which the highest number of visits was 3 331 (13.74%) among children of two months. Atopic dermatitis/eczema and allergic rhinitis had a higher number of visits with 46 156 and 14 388 visits, respectively. The age of onset of atopic dermatitis was earlier, with the age of first consultation being 0.42 years, and the age of onset was earlier in males than in females. Atopic dermatitis/eczema and allergic rhinitis were the most common multimorbidities. Conclusion For children with atopic dermatitis/eczema and/or allergic rhinitis, the focus is on the development of concomitant diseases to further interrupt the allergic process and promote early detection, prevention and treatment of the disease. [ABSTRACT FROM AUTHOR]

Objective To investigate the intervention effect of Akkermansia muciniphila (AKK) on atopic dermatitis (AD) induced by 2,4-dicMoronitrobenzene (DNCB) in mice and the effect of intestinal flora. Methods In total 72 BALB/c mice were randomly divided into high and low dose live bacteria group, pasteurization group, model group, and blank group, with 12 mice in each group. The AD mouse model was established by DNCB, and different doses of AKK living bacteria and Pas-teurella multicide were intragastrically administered for 4 weeks. The skin lesions, splenomegaly, and skin histopathology of mice in each group were observed, the level of serum IgE was measured, and the expression levels of IL-4 and IFN-y on mRNA transcription and protein in back skin tissue were detected. The fecal samples of mice were collected and 16SrRNA sequencing was used to understand the changes in the structure and abundance of intestinal microflora. Results After AKK intervention, the skin lesions, splenomegaly, and skin pathological damage of AD mice were not significantly improved, the level of serum IgE increased, the level of IL-4 decreased except the mRNA transcription level of low dose live bacteria group, and the expression of IFN-y increased at mRNA and protein levels in low dose live bacteria group and high dose inactivated group. Conclusion Although AKK did not significantly improve the skin lesions of AD mice, it alleviated the inflammatory reaction in mice to some extent and improved the diversity and structure of intestinal flora. [ABSTRACT FROM AUTHOR]