1. Serial propagation of the guinea pig salivary gland virus in tissue culture
- Author
-
Robert J. Huebner, Janet W. Hartley, and Wallace P. Rowe
- Subjects
medicine.medical_specialty ,biology ,Salivary gland ,Reproduction ,Cytomegalovirus ,Immunologic Tests ,Virology ,General Biochemistry, Genetics and Molecular Biology ,Virus ,Guinea pig ,Tissue culture ,medicine.anatomical_structure ,Research Design ,Viruses ,medicine ,biology.protein ,Histopathology ,Antibody ,Submaxillary gland ,Neutralizing antibody ,Roseolovirus - Abstract
SummaryThe guinea pig submaxillary gland virus has been successfully propagated in tissue culture, one strain having been carried through 22 serial passages. The tissue culture passage virus produced characteristic intranuclear inclusion bodies in tissue cultures, demonstrated complement fixing and neutralizing antibody responses in guinea pigs infected with animal passage virus, and produced characteristic disease and histopathology in susceptible guinea pigs. Commercial complement consistently contained high titer CF antibody.
- Published
- 1957