Man's relation to and dependence upon other forms of life are nowhere illustrated more vividly than in the field of his health. He has known for some time that certain of his most devastating diseases are transmitted to him by mosquitoes, fleas, lice, ticks, and various vertebrates. He is beginning to realize that in many cases these vectors obtain their initial infections from other vertebrate animals which are the natural hosts of the disease organisms. But although he is aware that many members of the animal kingdom thus form a tremendous disease reservoir which may serve as a continuing source of human infection (Meyer 1931), he is as yet largely ignorant of the complex epidemiological interrelationships which this fact implies. For a number of years the George Williams Hooper Foundation has directed research into various aspects of this problem. Part of the Foundation's program has been an investigation of the host-parasite-disease relationships existing in a natural mammalian community. The following report describes this investigation. No such study pretends to be complete or to do more than scratch the surface of this field of inquiry. However, in reporting on the ectoparasites obtained over six years from the maminals of a restricted area, we have tried to indicate some of the potentialities that such a community provides for the maintenance and spread of disease organisms dependent upon arthropod transmission. Throughout this study, attention was focused primarily on the California ground squirrel, Citellus beecheyi, which has long been known as a factor in the epidemiology of plague (Wherry 1908). This squirrel is one of the commonest rodents in California and is usually an important member of any natural community in which it is found; in many areas of the state it is a serious agricultural pest. It was accordingly selected for intensive study to determine its role as a reservoir of disease. In the choice of a site for this investigation, therefore, important considerations were an abundance of ground squirrels, an absence of human activity which would disturb natural conditions, and accessibility throughout the year. These prerequisites were provided by the environs of the Calaveras Dam, in the Livermore Hills of the central Coast Range, eight miles south of Sunol, Alameda County, California. The terrain is rugged there; some hills reach an elevation of 2000 feet, and the bottoms of some canyons are no more than 500 feet above sea level. Grassland predominates, with trees and brush growing chiefly on the northern exposures and in ravines. The climate of the region is somewhat arid. May, June, July, August, and September are largely rainless, and most of the precipitation occurs in November, December, January, and February. Rainfall in this latter period of the study varied from 17.4 to 25.2 inches per year. Conditions are influenced by the moderate weather prevailing on the coast. High morning fogs are frequent in early summer, and a heavy dew accumulates on the low vegetation nearly every night in April and May. The minimum and maximum temperatures recorded were 190 and 1090 F. respectively. Mean monthly temperatures of 65 0