1. Sterility Test Method for Petrolatum-Based Ophthalmic Ointments
- Author
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Tsuji, Kiyoshi, Stapert, E. M., Robertson, John H., and Waiyaki, Peter M.
- Abstract
A sensitive sterility testing procedure for the detection of microbial contamination in petrolatum-based ointments is described. The method involves dissolving the ointment in filter-sterilized isopropyl myristate and filtering through a membrane filter. Improved sensitivity is obtained by blending the membrane in Trypticase Soy Broth before incubation. Filter-sterilized isopropyl myristate is shown to be less toxic to microorganisms than heat-sterilized isopropyl myristate. The isopropyl myristate method is more sensitive than the polyethylene glycol-ether method for the detection of microbial contamination.
- Published
- 1970
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