1. Chemical synthesis of ppGpp
- Author
-
J. Tomasz and A. Simoncsits
- Subjects
Aqueous solution ,Stereochemistry ,Guanosine ,Hydrogen-Ion Concentration ,Alkaline Phosphatase ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Pyrophosphate ,Chemical synthesis ,Chromatography, DEAE-Cellulose ,Guanine Nucleotides ,Uridine ,Diphosphates ,Intestines ,chemistry.chemical_compound ,Acetic acid ,chemistry ,Evaluation Studies as Topic ,Functional group ,Escherichia coli ,Methods ,Animals ,Cattle ,Spectrophotometry, Ultraviolet ,Chromatography, Thin Layer ,Protecting group - Abstract
The chemical synthesis of ppGpp (guanosine 3′,5′-dipyrophosphate) is described. The synthesis started from guanosine 3′,5′-diphosphate. The acidlabile ethoxyethyl group was used to protect the 2′-OH functional group. The pyrophosphate linkages were built up simultaneously according to the anionexchange method and the protecting group was removed by dilute aqueous acetic acid. In an analogous manner the isomeric non-natural ppGpp (guanosine 2′,5′-dipyrophosphate) and uridine 2′(3′),5′-dipyrophosphate were synthesized as well.
- Published
- 1974
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