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The dose response curves of purified normal and chronic lymphocytic leukaemic lymphocytes to highly, purified phytohaemagglutinin in 3- and 6-day cultures have been defined. Normal lymphocytes cultured for 3 days were maximally stimulated by low concentrations of phytohaemagglutinin, in the range 0.4 to 1.4 μg phytohaemagglutinin/106 cells/ml. Higher concentrations of phytohaemagglutinin caused no further stimulation. Normal lymphocytes cultured for 6 days showed a progressively greater response to increasing concentrations of phytohaemaggluttnin up to 15 μg phytohaemagglutinin/106 cells/ml. Chronic lymphocytic leukaemic lymphocytes responded subnormally to all ranges of phytohaemagglutinin concentrations tested, m both 3- and 6-day cultures. The shape of the dose response curve resembled the normal curve in 2 of 5 cases of chronic lymphocytic leukaemic lymphocytes studied in 3-day cultures, and in all 7 cases studied in 8-day cultures. No evidence could be adduced of a consistently greater response of chronic lymphocytic leukaemic lymphocytes in 6-day compared with 3-day cultures. Five hours' exposure of normal lymphocytes to an optimum phytohaemagglutinin dose did not result in maximum H³-thymidine incorporation after 3 days in culture. [ABSTRACT FROM AUTHOR]

Three 1-yr-old swine and two 2.5-wk-old swine were fed a fat-free diet for 1 month and 5 months, respectively. The hepatic phosphatidylcholine and phosphatidylethanolamine were fractionated by silver ion thin-layer chromatography. A distinctive feature of the chromatographic procedure was the development of the chromatograms at low temperatures: -10 degrees C for phosphatidylcholine and 4 degrees C for phosphatidylethanolamine. The chromatographic fractions were hydrolyzed with phospholipase A(2), and the fatty acids were characterized. Significant concentrations of odd-chain saturated and unsaturated fatty acids were found in the swine deprived of fat for 5 months. The major molecular species of phosphatidylcholine in both groups contained monoenoic fatty acids: 16:0/18:1(n - 9), 18:0/18:1(n - 9), and 18:1(n - 9)/18:1(n - 9). Their concentrations changed only slightly with the diet. The molecular species of phosphatidylethanolamine were more sensitive to dietary changes. In the swine deprived of fat for 1 month, about 50% of the molecular species of phosphatidylethanolamine contained tetraenoic fatty acids: 16:0/20:4(n - 6), 18:0/20:4(n - 6), and 18:1(n - 9)/20:4(n - 6). The phosphatidylethanolamine of animals deprived of fat for 5 months contained only 3% molecular species with tetraenoic acids, 18:0/20:4(n - 6), but 36% molecular species with trienoic acids: 18:0/20:3(n - 9), 18:1(n - 9)/20:3(n - 9), 18:0/19:3(n - 8), 16:0/20:3(n - 9), and 17:0/20:3(n - 9). Doubly unsaturated species, such as 18:1(n - 9)/18:1(n - 9), 18:1(n - 9)/20:3(n - 9), and 18:1(n - 9)/20:4(n - 6), were found in both groups of swine, although their total concentrations were higher in the group deprived of fat for a longer period.