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6 results on '"SAM decarboxylase"'

3. S-adenosylmethionine synthetase in methionine regulatory mutants of Salmonella typhimurium

Author

Hobson, Ann C. and Smith, D. A.

Abstract

In wild-type bacteria, S-adenosylmethionine (SAM) synthetase activity was repressed by growth in methionine. MetJ regulatory mutants had elevated activities which did not show this repression. Two metK mutants with normal regulation of the methionine biosynthetic enzymes had elevated Km's (methionine) for SAM synthetase while five metK mutants with constitutive methionine enzymes showed no measurable SAM synthetase activity. One mutant, metKX721, similar in phenotype to these five had a wild-type level of SAM synthetase in conditions where SAM decarboxylase activity was blocked. By using an F'-factor carrying the metK region of the genome, this mutant was shown to complement six other metK mutants.

Published
1973
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5. S-adenosylmethionine synthetase in methionine regulatory mutants of Salmonella typhimurium

Author

Ann C. Hobson and Derek A. Smith

Subjects
Salmonella typhimurium, S-Adenosylmethionine, Salmonella, Carboxy-Lyases, Spermidine, Mutant, Biology, medicine.disease_cause, chemistry.chemical_compound, Adenosine Triphosphate, Methionine, S-Adenosylmethionine Synthetase, Transferases, Genes, Regulator, Genetics, medicine, Ultrasonics, Carbon Radioisotopes, Molecular Biology, Psychological repression, chemistry.chemical_classification, Genetic Complementation Test, Chromatography, Ion Exchange, biology.organism_classification, Molecular biology, Phenotype, Kinetics, Enzyme, chemistry, Biochemistry, Mutation, Enzyme Repression, Bacteria
Abstract

In wild-type bacteria, S-adenosylmethionine (SAM) synthetase activity was repressed by growth in methionine. MetJ regulatory mutants had elevated activities which did not show this repression. Two metK mutants with normal regulation of the methionine biosynthetic enzymes had elevated Km's (methionine) for SAM synthetase while five metK mutants with constitutive methionine enzymes showed no measurable SAM synthetase activity. One mutant, metK X 721, similar in phenotype to these five had a wild-type level of SAM synthetase in conditions where SAM decarboxylase activity was blocked. By using an F′-factor carrying the metK region of the genome, this mutant was shown to complement six other metK mutants. These results indicate that SAM or a derivative of it, rather than methionine itself, is the co-repressor of the methionine system, regulatory abnormalities resulting from the absence or reduction of the amount of SAM formed by the SAM synthetase reaction. As SAM is essential for bacteria it is likely that there is some alternative biosynthetic route for SAM.

Published
1973
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6. Organizational Biosynthesis

Author

Henry Vogel and Henry Vogel

Subjects
Proteins, Molecular biology--Congresses, Biosynthesis--Congresses
Abstract

Organizational Biosynthesis focuses on the organized biosynthesis of the bacterial chromosome and examines the fundamental aspects of this process. This book discusses the basic particles of biology, which are explored in terms of organizational biosynthetic events at the molecular level. Organized into 10 parts encompassing 45 chapters, this book starts with an overview of how cellular components recognize, coordinate, and interact with each other during cellular division and genetic recombination. This text then examines the biochemical experiments with bacteria, which strongly suggest the participation of a particulate fraction. Other chapters consider the molecular basis for the physical continuity of the chromosome. This book discusses as well the result of the experiments for bacteria, which shows that DNA replication can proceed only after both template strands of the replicating molecule attach to the cell. The final chapter deals with protein biosynthesis system in mitochondria. This book is a valuable resource for biologists and biochemists.

Published
1967

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